Effects of Alfalfa Nitrogen Efficiency Differences on Nutrient Characteristics and Microbial Community Characteristics of Rhizosphere Soil

doi:10.11733/j.issn.1007-0435.2025.07.011

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (7): 2150-2161.DOI: 10.11733/j.issn.1007-0435.2025.07.011

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Effects of Alfalfa Nitrogen Efficiency Differences on Nutrient Characteristics and Microbial Community Characteristics of Rhizosphere Soil

HE Sheng-ran¹, LIU Xiao-jing¹, HAN Tian-hu², ZHAO Ya-jiao¹, HAN Yan-long¹

1. Pratacultural College, Gansu Agricultural University, Key Laboratory of Pratacultural Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, The Sino-U. S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
2. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730020, China

Received:2024-09-28 Revised:2025-01-27 Online:2025-07-15 Published:2025-07-18

紫花苜蓿氮效率差异对其根际土壤养分特性及微生物群落特征的影响

何升然¹, 刘晓静¹, 韩天虎², 赵雅姣¹, 韩颜隆¹

1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070;
2. 兰州大学草地农业科技学院, 甘肃兰州 730020

通讯作者: 刘晓静,E-mail:liuxj@gsau.edu.cn
作者简介:何升然（1998-），男，汉族，安徽霍山人，硕士研究生，主要从事牧草栽培与利用研究，E-mail：949561991@qq.com
基金资助:
国家自然科学基金项目（32171674）；兰州市人才创新创业项目（2023-RC-40）；甘肃省重点研发计划项目（20YF8NA130）资助

Abstract

Abstract: To explore the effects of different nitrogen efficiency of alfalfa on nutrient characteristics and microbial community characteristics of rhizosphere soil， this study took alfalfa with high nitrogen efficiency （‘LW6010’） and alfalfa with low nitrogen efficiency （‘Longdong’ alfalfa） as experimental materials， and studied the effects of nitrogen application on its rhizosphere soil nutrients and enzymes under the conditions of no nitrogen （N₀） and 103.5 kg·hm^-2（pure N）. The results showed that under two nitrogen levels， the rhizosphere soil total nitrogen， available nitrogen， organic matter， microbial biomass carbon content and enzyme activity of nitrogen-efficient type alfalfa ‘LW6010’ were significantly higher than those of nitrogen-inefficient alfalfa type ‘Longdong’ alfalfa， while pH value was significantly lower than ‘Longdong’ alfalfa （P<0.05）. Under the treatment of nitrogen application， the richness and diversity index of rhizosphere soil bacterial community of nitrogen-efficient type alfalfa ‘LW6010’ were higher than those of nitrogen-inefficient type alfalfa ‘Longdong’ alfalfa. In summary， nutrient content and soil enzyme activity of nitrogen-efficient alfalfa ‘LW6010’ were significantly higher than those of nitrogen-inefficient alfalfa ‘Longdong’ alfalfa， and the abundance of beneficial bacteria， such as Proteobacteria， Bacteroidetes and Pseudomonas， etc.， in rhizosphere soil of ‘LW6010’ was higher than that of ‘Longdong’ alfalfa， which was more conducive to improving soil fertility.

Key words: Alfalfa, Nitrogen efficiency type, Rhizosphere soil nutrients, Rhizosphere microbial community characteristics

摘要： 为探究紫花苜蓿（Medicago sativa）氮效率差异对其根际土壤养分特性及微生物群落特征的影响，本研究以氮高效型紫花苜蓿（‘LW6010’）和氮低效型紫花苜蓿（‘陇东’苜蓿）为试验材料，在不施氮（N₀）和施氮103.5 kg·hm^-2（纯N）条件下对其根际土壤养分、酶活性及微生物群落特征的差异进行了研究。结果表明：氮高效型紫花苜蓿‘LW6010’的根际土壤全氮、碱解氮、有机质、微生物量碳含量和土壤酶活性均显著高于‘陇东’苜蓿，而pH值显著低于‘陇东’苜蓿（P<0.05）。在施氮处理下，紫花苜蓿‘LW6010’的根际土壤细菌群落丰富度和多样性指数均高于‘陇东’苜蓿。综上所述，氮高效型紫花苜蓿‘LW6010’的根际土壤养分含量和土壤酶活性均显著高于氮低效型‘陇东’苜蓿，且‘LW6010’的根际土壤变形菌门、拟杆菌门和假单胞菌属等有益菌群的丰度均高于‘陇东’苜蓿，更有利于提高土壤肥力。

关键词: 紫花苜蓿, 氮效率类型, 根际土壤养分, 根际微生物群落特征

CLC Number:

S541.9

HE Sheng-ran, LIU Xiao-jing, HAN Tian-hu, ZHAO Ya-jiao, HAN Yan-long. Effects of Alfalfa Nitrogen Efficiency Differences on Nutrient Characteristics and Microbial Community Characteristics of Rhizosphere Soil[J]. Acta Agrestia Sinica, 2025, 33(7): 2150-2161.

何升然, 刘晓静, 韩天虎, 赵雅姣, 韩颜隆. 紫花苜蓿氮效率差异对其根际土壤养分特性及微生物群落特征的影响[J]. 草地学报, 2025, 33(7): 2150-2161.

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Effects of Alfalfa Nitrogen Efficiency Differences on Nutrient Characteristics and Microbial Community Characteristics of Rhizosphere Soil

紫花苜蓿氮效率差异对其根际土壤养分特性及微生物群落特征的影响

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