Comprehensive Evaluation of Salt Tolerance of 23 Alfalfa Materials during Seeds Germination Stage

doi:10.11733/j.issn.1007-0435.2025.07.020

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (7): 2249-2261.DOI: 10.11733/j.issn.1007-0435.2025.07.020

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Comprehensive Evaluation of Salt Tolerance of 23 Alfalfa Materials during Seeds Germination Stage

ZENG Yi, YUAN Jia-miao, YANG Jing, WANG Yu-xiang

Key Laboratory of Grassland Resources and Ecology of Ministry of Education in Western Dry Desert Region, Key Laboratory of Grassland Resources and Ecology of Xinjiang, College of Grassland Industry, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China

Received:2024-10-10 Revised:2024-12-11 Online:2025-07-15 Published:2025-07-18

23份紫花苜蓿材料萌发期的耐盐性综合评价

曾怡, 袁嘉苗, 杨静, 王玉祥

西部干旱荒漠区草地资源与生态教育部重点实验室, 新疆草地资源与生态重点实验室, 新疆农业大学草业学院, 新疆乌鲁木齐 830052

通讯作者: 王玉祥,E-mail:wyx9868@163.com
作者简介:曾怡（1999-），女，汉族，河南焦作人，硕士研究生，主要从事草种质资源与育种研究，E-mail：384159586@qq.com
基金资助:
农业部苜蓿育种专项（2022ZD0401104）；新疆维吾尔自治区科技重大专项（2022A03004）共同资助

Abstract

Abstract: To explore salt tolerance of alfalfa during seeds germination period， 18 alfalfa （Medicago sativa L.） germplasm resources and 5 cultivated varieties were used to study the effects of NaCl solution with different conductivity （Electrical，EC） values on seeds germination rate， germination potential and germination index during seed germination period. The salt resistance of the test materials was evaluated by principal component analyses and membership function.The results showed that the germination response of different alfalfa materials showed different trends， and the performance of the indexes was quite different.The 23 materials were evaluvated comprehensively by using salt tolerance coefficient and comprehensive evaluation D value and systematic cluster analysis. Based on D value， the 23 materials could be divided into 5 categories： Class I with 4 materials with D value of 0.760， category 8 materials with D value of 0.670， category 6 materials with D value of 0.536， category 4 materials with a D value of 0.430. Class V with only 1 material with D value of 0.241. Among the 23 materials tested， A1Z 003， A1Z 016， A1Z 005， etc.； A1Z 001， A1Z 006， A1Z 015， etc. Through principal component and correlation analysis， germination rate， germination potential and germination index can be selected as the main indicators of salt tolerance during seeds germination period. This study can provide a theoretical reference for the breeding of saline-alkali tolerant alfalfa varieties.

Key words: Germination, Salt stress, Conductivity, Membership function

摘要： 为探究紫花苜蓿不同种质材料种子萌发期的耐盐性，以18份紫花苜蓿（Medicago sativa L.）种质资源和5份育成品种为材料，研究不同电导率（（Electrical，EC）值的NaCl溶液对种子萌发期的发芽率、发芽势及发芽指数等指标的影响。采用主成分分析和隶属函数等方法对供试材料进行耐盐性评价。结果表明：不同紫花苜蓿材料萌发期对盐胁迫响应呈现出不同的变化趋势，各项指标表现差异较大。利用耐盐系数和综合评价D值对23份材料进行综合性评价，并基于D值进行系统聚类分析，可将23份材料分为5类：第Ⅰ类有4个材料，D值为0.760；第Ⅱ类8个材料，D值为0.670；第Ⅲ类6个材料，D值为0.536；第Ⅳ类4个材料，D值为0.430；第Ⅴ类只有1个材料，D值为0.241。在供试的23份材料中，除5个育成品种外，综合筛选出耐盐性较强的材料有A1Z 003，A1Z 016，A1Z 005等；耐盐性较弱的材料有A1Z 001，A1Z 006，A1Z 015等。通过主成分和相关性分析，筛选出发芽率、发芽势和发芽指数可作为萌发期耐盐性鉴定的主要指标。本研究可为盐碱地苜蓿品种选育提供理论参考依据。

关键词: 萌发期, 盐胁迫, 电导率, 隶属函数

CLC Number:

S332.6

ZENG Yi, YUAN Jia-miao, YANG Jing, WANG Yu-xiang. Comprehensive Evaluation of Salt Tolerance of 23 Alfalfa Materials during Seeds Germination Stage[J]. Acta Agrestia Sinica, 2025, 33(7): 2249-2261.

曾怡, 袁嘉苗, 杨静, 王玉祥. 23份紫花苜蓿材料萌发期的耐盐性综合评价[J]. 草地学报, 2025, 33(7): 2249-2261.

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Comprehensive Evaluation of Salt Tolerance of 23 Alfalfa Materials during Seeds Germination Stage

23份紫花苜蓿材料萌发期的耐盐性综合评价

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