Effect of MsTIFY10a Gene on Cold Resistance of Transgenic Alfalfa Plants

doi:10.11733/j.issn.1007-0435.2025.09.004

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (9): 2786-2795.DOI: 10.11733/j.issn.1007-0435.2025.09.004

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Effect of MsTIFY10a Gene on Cold Resistance of Transgenic Alfalfa Plants

CHEN Qi¹, ZHANG Yan¹, HUO Xiao-wei¹, YI Feng-yan², SHI Feng-ling¹, GAO Cui-ping¹, LI Zhen-yi¹, ZHANG Zhi-qiang¹

1. Key Laboratory of Grassland Resources, College of Grassland, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China;
2. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, Inner Mongolia 010010, China

Received:2025-02-20 Revised:2025-03-23 Online:2025-09-15 Published:2025-09-22

紫花苜蓿MsTIFY10a基因对植物抗寒性的影响

陈崎¹, 张岩¹, 霍晓伟¹, 伊风艳², 石凤翎¹, 高翠萍¹, 栗振义¹, 张志强¹

1. 内蒙古农业大学草业学院, 草地资源教育部重点实验室, 内蒙古呼和浩特 010018;
2. 内蒙古农牧业科学院, 内蒙古呼和浩特 010010

通讯作者: 栗振义，E-mail：lizhenyily@163.com；张志强，E-mail：zhangzq1989@imau.edu.cn
作者简介:陈崎（1999-），女，汉族，内蒙古乌拉特前旗人，博士研究生，主要从事草育种方面研究，E-mail：ndcq@emails.imau.edu.cn
基金资助:
国家自然科学基金项目（32460341）；内蒙古自治区高等学校青年科技英才支持项目（NJYT23009）；苜蓿分子育种体系构建及种质创制（BR22-11-12）；内蒙古农业大学一流学科专项（YLXKZX-NND-033）资助

Abstract

Abstract: Cold stress is one of the important limiting factors affecting the yield and quality of alfalfa and its popularization in cold areas. Jasmonate （JA） plays an important role in plant growth and development and cold stress response. The objective of this study was to investigate the function of MsTIFY10a in cold resistance， a key regulatory gene of jasmonic acid pathway in alfalfa. In this study， MsTIFY10a transgenic and wild-type Medicago truncatula was used to determine the phenotype， physiological and biochemical， photosynthetic capacity and expression levels of related genes under cold stress. The results showed that the contents of soluble sugar and soluble protein， activities of SOD， POD and CAT， chlorophyll content and photosynthetic index of transgenic MsTIFY10a transgenic plants were higher than those of wild-type under cold stress， and the expressions of cold resistance genes， including MtCML10， MtNAC and MtAOC2 were significantly up-regulated. Cold tolerance was increased in MsTIFY10a transgenic plants by increasing the content of osmoregulatory substances， activity of antioxidant enzymes and photosynthetic capacity， and up-regulating the expression of cold resistant genes.

Key words: Medicago sativa, MsTIFY10a gene, Cold resistance, Physiology, JA

摘要： 低温是影响苜蓿产量和品质及其寒区推广的重要限制因子之一。茉莉酸（Jasmonate， JA）在植物生长发育和低温响应过程中具有重要作用。本研究旨在探究紫花苜蓿茉莉酸途径关键调控基因MsTIFY10a在抗寒防御反应中的作用。以MsTIFY10a过表达和野生型蒺藜苜蓿为材料，进行低温胁迫下表型、生理生化、光合能力及相关基因表达量的测定。结果表明：低温胁迫下，MsTIFY10a转基因植株的可溶性糖和可溶性蛋白含量，SOD、POD、CAT活性，叶绿素含量以及光合指标均高于野生型植株，低温响应基因MtCML10、MtNAC和MtAOC2表达量均显著上调。MsTIFY10a通过提升渗透调节物质含量、抗氧化酶活性和光合能力，上调抗寒基因表达，提高苜蓿的耐寒性。

关键词: 紫花苜蓿, MsTIFY10a基因, 抗寒性, 生理, 茉莉酸

CLC Number:

S332.6

CHEN Qi, ZHANG Yan, HUO Xiao-wei, YI Feng-yan, SHI Feng-ling, GAO Cui-ping, LI Zhen-yi, ZHANG Zhi-qiang. Effect of MsTIFY10a Gene on Cold Resistance of Transgenic Alfalfa Plants[J]. Acta Agrestia Sinica, 2025, 33(9): 2786-2795.

陈崎, 张岩, 霍晓伟, 伊风艳, 石凤翎, 高翠萍, 栗振义, 张志强. 紫花苜蓿MsTIFY10a基因对植物抗寒性的影响[J]. 草地学报, 2025, 33(9): 2786-2795.

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Effect of MsTIFY10a Gene on Cold Resistance of Transgenic Alfalfa Plants

紫花苜蓿MsTIFY10a基因对植物抗寒性的影响

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