Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 1-9.DOI: 10.11733/j.issn.1007-0435.2026.01.001

   

Response of Root-Secreted Organic Acids in Thinopyrum ponticum to Varying Intensities of Salt Stress

ZHANG Ran1,2,3, YANG Liang1,2,3, LI Dan1,2,3, LI Yan-jie1,2,3, LU Ying-shuai1,2,3, CHEN Xiao-peng1,2,3   

  1. 1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
    2. Shanxi Key Laboratory of Grassland Ecological Conservation and Native Grass Germplasm Innovation, Taigu, Shanxi Province 030801, China;
    3. National Observation and Research Station for the Grassland Ecosystem on the Loess Plateau in Youyu, Youyu, Shanxi Province 030801, China
  • Received:2025-04-03 Revised:2025-06-27 Published:2025-12-24

盐胁迫对长穗偃麦草根系分泌有机酸的影响

张冉1,2,3, 杨亮1,2,3, 李丹1,2,3, 李彦颉1,2,3, 卢英帅1,2,3, 陈晓鹏1,2,3   

  1. 1. 山西农业大学草业学院, 山西 太谷 030801;
    2. 草地生态保护与乡土草种质创新山西省重点实验室, 山西 太谷 030801;
    3. 山西右玉黄土高原草地生态系统国家定位观测研究站, 山西 右玉 037200
  • 通讯作者: 陈晓鹏,E-mail:chenxp@sxau.edu.cn
  • 作者简介:张冉（2000-），男，蒙古族，内蒙古赤峰人，硕士研究生，主要从事草地生态学研究，E-mail:z17547965859@163.com；
  • 基金资助:
    国家自然科学基金项目（32271682）资助

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Abstract: To elucidate the responses of organic acids secreted by the roots of Thinopyrum ponticum under salt stress and their interactions with root traits and rhizosphere soil physicochemical properties， a controlled experiment with four salinity treatments was established： control， mild， moderate and severe salt stress （with soil salt contents of 0%， 0.2%， 0.4%， and 0.6% w/w， respectively）. Untargeted metabolomics was employed to analyze relative abundance variations and regulatory mechanisms of organic acids in root exudates. The results demonstrated that salt stress significantly decreased the secretion of organic acids including 1-hydroxy-2-naphthoic acid， succinic acid， homogentisic acid， phenylpyruvic acid and indolelactic acid （P<0.05）， while significantly enhancing the secretion of phenoxyacetic acid （P<0.05）. However， no significant effects on the secretion of 3-hydroxybutyric acid and shikimic acid. Principal coordinate analysis （PCoA） revealed significant differences in root-secreted organic acid profiles between control and salt-stressed treatments （mild， moderate and severe salinity） （P<0.05）. Correlation analysis demonstrated that soil salinity， electrical conductivity， water content， and pH were key determinants influencing root organic acid secretion. Path analysis further revealed that salt stress promoted phenoxyacetic acid secretion through soil salinization-mediated suppression of root morphological traits. These findings provide a theoretical basis for the in-depth analysis of the physiological mechanisms of plant salt tolerance.

Key words: Salinization, Salt tolerance mechanism, Rhizosphere effect, Root exudates

摘要： 为探究长穗偃麦草（Thinopyrum ponticum）根系分泌有机酸对盐胁迫的响应及其与根系性状和根际土壤理化性质的关系，设置对照、轻度、中度和重度（土壤含盐量分别为0%，0.2%，0.4%，0.6%）盐胁迫处理，利用非靶向代谢组学技术，分析长穗偃麦草根系分泌有机酸的相对丰度及其变化机制。结果表明，盐胁迫显著降低1-羟基-2-萘酸、琥珀酸、尿黑酸、苯丙酮酸及吲哚乳酸有机酸的分泌量（P<0.05），显著促进苯氧基乙酸的分泌（P<0.05），但对3-羟基丁酸和莽草酸的分泌无显著影响。主坐标分析显示，轻度、中度和重度盐胁迫处理的根系分泌有机酸与对照相比差异显著（P<0.05）。相关性分析表明，土壤盐度、电导率、含水量和pH是影响根系分泌有机酸的重要因素。路径分析结果表明，盐胁迫通过显著增加土壤盐分，抑制根系性状进而促进苯氧基乙酸的分泌。上述结果为深入解析植物耐盐生理机制提供理论依据。

关键词: 盐渍化, 耐盐机制, 根际效应, 根系分泌物

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