Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 33-42.DOI: 10.11733/j.issn.1007-0435.2026.01.004

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Cloning， Subcellular Localization and Expression Analysis of MsMYB44 Gene of Medicago sativa‘Pianguan’

LI Di-na1, WANG Kai1, TAO Jia-li1,2, XU Tao1, LI Qing-xiu1, ZHU Hui-sen1, CEN Hui-fang1   

  1. 1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
    2. Department of Mining Engineering, Shanxi Institute of Technology, Yangquan, Shanxi Province 045000, China
  • Received:2024-12-27 Revised:2025-03-30 Published:2025-12-24

偏关苜蓿MsMYB44基因的克隆、亚细胞定位及表达分析

李迪娜1, 王凯1, 陶佳丽1,2, 许涛1, 李青秀1, 朱慧森1, 岑慧芳1   

  1. 1. 山西农业大学草业学院, 山西 太谷 030801;
    2. 山西工程技术学院矿业工程系, 山西 阳泉 045000
  • 通讯作者: 朱慧森,E-mail:zhuhuisen@126.com
  • 作者简介:李迪娜（1999-），女，汉族，黑龙江哈尔滨人，硕士研究生，主要从事牧草种质资源与育种相关研究，E-mail:13354593936@163.com；
  • 基金资助:
    国家自然科学基金项目（32071872）；山西省中央引导地方科技发展资金项目（YDZJSX2022B006）；山西省重点研发计划课题（202102140601006-3）；山西省基础研究计划（202303021221101）；山西省来晋科研奖励（SXBYKY2022118）；优秀博士启动项目（2021BQ01，2023BQ03）资助

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Abstract: The MYB （V-myb Avian myeloblastosis viral Oncogene Homolog） gene family represents one of the most extensive groups of transcription factors in plants， playing a crucial role in various processes including plant growth， development， and stress responses. This study utilized Medicago sativa‘Pianguan’ to investigate the characteristics of the MsMYB44 gene through methodologies such as gene cloning， bioinformatics analysis， subcellular localization， and expression pattern analysis. The findings revealed that the MsMYB44 gene of Medicago sativa‘Pianguan’ comprised 966 base pairs and encoded a total of 321 amino acids. The MsMYB44 protein exhibited a high degree of homology to the MtMYB44 protein in Medicago truncatula and was localized within the nucleus. The expression of the MsMYB44 gene was observed in various tissues of Medicago sativa‘Pianguan’ across different developmental stages， indicating tissue-specific expression patterns. The MsMYB44 gene was responsive to drought， salt stress， and abscisic acid （ABA） treatment. Under drought conditions， the relative expression level of MsMYB44 initially increased， subsequently decreased， and then increased again， peaking at 12 hours. In response to salt stress， the relative expression level of MsMYB44 gradually increased after 48 hours， reaching its maximum at 72 hours. Following ABA treatment， the relative expression level of MsMYB44 peaked at 12 hours and then progressively declined. These findings would provide a theoretical framework for elucidating the role of the MsMYB44 gene of Medicago sativa in mediating the abiotic stress response.

Key words: MsMYB44, Medicago sativa‘Pianguan’, Cloning identification, Subcellular localization, Expression pattern, Abiotic stress

摘要： MYB（V-myb Avian myeloblastosis viral Oncogene Homolog）基因家族是植物中最大的转录因子家族之一，广泛参与植物的生长发育和逆境胁迫等过程。本研究以‘偏关’苜蓿（Medicago sativa ‘Pianguan’）为材料，通过基因克隆、生物信息学分析、亚细胞定位及表达模式分析来探究MsMYB44基因的特性。结果显示：‘偏关’苜蓿MsMYB44基因长966 bp，共编码321个氨基酸。MsMYB44蛋白与蒺藜苜蓿（Medicago truncatula）MtMYB44蛋白高度同源且定位于细胞核内。MsMYB44基因在‘偏关’苜蓿不同生育期的不同部位均有表达，表现出组织表达特异性。MsMYB44基因对干旱、盐胁迫及ABA处理均有响应。干旱胁迫下，MsMYB44相对表达量呈现出先升高后降低再升高的趋势，并在12 h时达到峰值；盐胁迫下，MsMYB44相对表达量在48 h后逐渐升高，且在72 h时达到最高值；ABA处理下，MsMYB44在12 h时相对表达量最高，随后逐渐降低。研究结果有望为阐释MsMYB44基因调控紫花苜蓿非生物胁迫响应机制提供一定理论参考。

关键词: MsMYB44, ‘偏关’苜蓿, 克隆鉴定, 亚细胞定位, 表达模式, 非生物胁迫

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