A Comparative Study on the Accumulation Characteristics of Osmolytes in Two Sweet Sorghum Cultivars under PEG Treatments

doi:10.11733/j.issn.1007-0435.2026.10.011

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (10): 3642-3652.DOI: 10.11733/j.issn.1007-0435.2026.10.011

A Comparative Study on the Accumulation Characteristics of Osmolytes in Two Sweet Sorghum Cultivars under PEG Treatments

YAN Shi-jie, XU Jiu-hui, ZHAO Xin, MA Zi-chen, ZHAO Ying-jie, LIU Jia, YANG Hao-ru, MA Yue, CUI Yan-nong, CAO Yu-man

College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China

Received:2025-12-22 Revised:2026-01-23 Published:2026-10-10

PEG处理下两个甜高粱品种渗透调节物质积累特征比较研究

闫世杰, 徐九晖, 赵鑫, 马紫晨, 赵英杰, 刘佳, 杨浩儒, 马樾, 崔彦农, 曹玉曼

西北农林科技大学草业与草原学院, 陕西杨凌 712100

通讯作者: 崔彦农, E-mail: cuiyn@nwafu.edu.cn;曹玉曼, E-mail: yumancao@nwafu.edu.cn
作者简介:闫世杰（2001-），男，汉族，山西运城人，硕士研究生，主要从事牧草逆境生理与分子生物学研究，E-mail：yjay@nwafu.edu.cn
基金资助:
国家自然科学基金（32301505）；中国博士后科学基金（2024M762652）；大学生创新创业训练计划项目（S202410712691；202510712065）资助

Abstract

Abstract: Osmotic adjustment is a key strategy of plants to cope with water deficit. To investigate the osmotic adjustment mechanisms of sweet sorghum under drought stresses， the growth， photosynthesis and accumulation of osmolytes in two sweet sorghum cultivars ‘Lvjuren’ and ‘Fengtian’ under 10%，20% and 30% PEG treatments were analyzed. Then， the expression of genes involved in K⁺ and NO₃^- transport as well as biosynthesis of organic osmolytes after 20% PEG treatment were determined. The results showed that the decline of growth and photosynthesis related parameters in ‘Fengtain’ was significantly smaller than that in ‘Lvjuren’， while the leaf K⁺，NO₃^-， soluble sugars and betaine contents in the former were significantly higher than those in the latter under PEG treatments. After 20% PEG treatment for 6 and 48 h， the expressions AKT1 and SKOR （mediating K⁺ transport）， NPF7.3 （mediating NO₃^- transport）， SUS1 （involved in sugar metabolism）， TPS6 （involved in trehalose biosynthesis）， RAFS2 （involved in raffinose biosynthesis）， BADH2 （involved in betaine biosynthesis） and BCAT2 （catalyzing the biosynthesis of branched-chain amino acids） in ‘Fengtian’ were significantly higher than those in ‘Lvjuren’， while the free proline content as well as the expressions of P5CS2 and P5CR （involved in proline biosynthesis） in the former were significantly lower than those in the latter. The above results provide a theoretical basis for uncovering the drought resistance mechanisms of sweet sorghum and large-scale cultivation of this species in arid and semi-arid regions.

Key words: Drought stress, Sweet sorghum, Osmolyte, Gene expression

摘要： 为解析干旱胁迫下甜高粱[Sorghum bicolor（L.）Moench]渗透调节机制，本研究以甜高粱品种‘绿巨人’和‘丰甜’为材料，比较了不同浓度（10%，20%，30%）聚乙二醇（Polyethylene glycol，PEG）处理下二者生长、光合性能和渗透调节物质积累的差异，分析了20% PEG处理下K⁺和NO₃^-转运以及有机渗透调节物合成关键基因的表达情况。结果表明，PEG处理下‘丰甜’生长及光合作用相关指标下降幅度显著小于‘绿巨人’，且前者叶中K⁺、NO₃^-、可溶性糖和甜菜碱含量显著高于后者。20% PEG处理6 h和48 h后，‘丰甜’K⁺转运基因AKT1和SKOR、NO₃^-转运基因NPF7.3、蔗糖代谢基因SUS1、海藻糖合成基因TPS6、棉子糖合成基因RAFS2、甜菜碱合成基因BADH2以及支链氨基酸合成关键基因BCAT2的表达量显著高于‘绿巨人’，但前者叶中游离脯氨酸含量及其合成关键基因P5CS2和P5CR的表达量均显著低于后者。上述结果为揭示甜高粱抗旱机制提供了理论依据。

关键词: 干旱胁迫, 甜高粱, 渗透调节物质, 基因表达

CLC Number:

S544.9

YAN Shi-jie, XU Jiu-hui, ZHAO Xin, MA Zi-chen, ZHAO Ying-jie, LIU Jia, YANG Hao-ru, MA Yue, CUI Yan-nong, CAO Yu-man. A Comparative Study on the Accumulation Characteristics of Osmolytes in Two Sweet Sorghum Cultivars under PEG Treatments[J]. Acta Agrestia Sinica, 2026, 34(10): 3642-3652.

闫世杰, 徐九晖, 赵鑫, 马紫晨, 赵英杰, 刘佳, 杨浩儒, 马樾, 崔彦农, 曹玉曼. PEG处理下两个甜高粱品种渗透调节物质积累特征比较研究[J]. 草地学报, 2026, 34(10): 3642-3652.

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A Comparative Study on the Accumulation Characteristics of Osmolytes in Two Sweet Sorghum Cultivars under PEG Treatments

PEG处理下两个甜高粱品种渗透调节物质积累特征比较研究

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