Research Advances and Breeding Prospects on Alfalfa Flowering Time Regulation

doi:10.11733/j.issn.1007-0435.2026.02.001

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (2): 377-391.DOI: 10.11733/j.issn.1007-0435.2026.02.001

Research Advances and Breeding Prospects on Alfalfa Flowering Time Regulation

LIU Rui-chao, YU Lin-qing, REN Hui-min, WU Xiao-feng, CUI Le-le

1. Key Laboratory of Forage and Special Crop Biology, Ministry of Education, College of Life Sciences, Inner Mongolia University, Hohhot, Inner Mongolia 010000, China

Received:2025-03-13 Revised:2025-05-23 Published:2026-01-22

苜蓿花期调控研究进展及育种展望

刘睿超, 于林清, 任慧敏, 武啸峰, 崔乐乐

1. 牧草与特色作物生物学教育部重点实验室, 内蒙古大学生命科学学院, 内蒙古呼和浩特 010000

通讯作者: 于林清，E-mail:linqing_yu126@.com
作者简介:刘睿超（1997-），女，汉族，内蒙古巴彦淖尔人，硕士研究生，主要从事牧草育种研究，E-mail:liuruichaonnd@163.com；
基金资助:
内蒙古自治区科技计划项目“河套地区苜蓿抗旱耐盐碱品种筛选及其配套优质丰产栽培技术研究与示范”（2023YFHH0083）；内蒙古自治区“揭榜挂帅”项目“优质苜蓿新品种选育及产业化示范”（2022JBGS0020）；“科技创新2030一重大项目子课题”（2022ZD040110302）资助

Abstract

Abstract: Alfalfa （Medicago sativa L.） is a globally important leguminous forage crop， widely cultivated and utilized due to its high biomass yield， nutritional value， strong adaptability， and excellent palatability. Flowering time is a critical stage in alfalfa growth cycle， directly affecting forage yield and quality. However， variations in environmental factors and genetic characteristics often lead to significant differences in flowering time， which in turn affecting its production， cultivation， management， and harvesting efficiency. In recent years， significant progresses have been made in the field of molecular biology， revealing the regulatory mechanisms of flowering gene expression was influenced by photoperiod， temperature and hormones. However， in China， the research on alfalfa flowering time regulation remains insufficient， with challenges including weak basic research， outdated breeding techniques， and inconsistent flowering periods among cultivars. This review summarizes the recent advances in the regulation of alfalfa flowering time， explores the ecological factors and genetic mechanisms influencing flowering， and proposes future optimization strategies for breeding and cultivation management. The aim is to provide theoretical support and practical guidance for high-yield， high-quality alfalfa breeding and sustainable utilization.

Key words: Alfalfa, Flowering time, Growth cycle, Flowering genes, Breeding

摘要： 苜蓿（Medicago sativa L.）是全球重要的豆科牧草，因其生物量高，营养价值高，适应性强，适口性好而被广泛种植和利用。开花时间是苜蓿生育期的关键阶段，直接影响牧草产量及品质。但是，由于环境因素和遗传特性不同，苜蓿花期常表现出较大的差异性，影响其生产栽培和收获效率。近年来，学术界在分子生物学研究方向取得了一定的研究进展，揭示了光周期、温度、激素等因素对开花基因表达的调控机制。目前我国在苜蓿花期研究方面仍存在基础研究薄弱、育种技术落后、品种花期不规范等问题。本文综述了苜蓿花期调控的研究进展，探讨了影响苜蓿花期的生态因子及基因调控机制，并提出了未来育种和栽培管理的优化策略，以期为苜蓿高产优质育种和可持续利用提供理论支持和实践指导。

关键词: 苜蓿, 开花时间, 生育期, 开花基因, 育种

LIU Rui-chao, YU Lin-qing, REN Hui-min, WU Xiao-feng, CUI Le-le. Research Advances and Breeding Prospects on Alfalfa Flowering Time Regulation[J]. Acta Agrestia Sinica, 2026, 34(2): 377-391.

刘睿超, 于林清, 任慧敏, 武啸峰, 崔乐乐. 苜蓿花期调控研究进展及育种展望[J]. 草地学报, 2026, 34(2): 377-391.

References

[1] JUNG C， ANDREAS E.MÜLLER. Flowering time control and applications in plant breeding[J]. Trends in Plant Science，2009，14（10）：563-573
[2] AUNG B； GRUBER M； L AMYOTet al. Ectopic expression of LjmiR156 delays flowering， enhances shoot branching， and improves forage quality in alfalfa. [J]. Plant Biotechnol Report 2015，9（6）：379-393
[3] SYKOVÁ E V A，JENDELOVÁ P. Magnetic resonance tracking of implanted adult and embryonic stem cells in injured brain and spinal cord[J]. Annals of the New York Academy of Sciences，2005，1049（1）：146-160
[4] CAI Y，WANG L，CHEN L，et al. Mutagenesis of GmFT2a and GmFT5a mediated by CRISPR/Cas9 contributes for expanding the regional adaptability of soybean[J]. Plant Biotechnology Journal，2020，18（1）：298-309
[5] CHENG X，LI G，KROM N，et al. Genetic regulation of flowering time and inflorescence architecture by MtFDa and MtFTa1 in Medicago truncatula[J]. Plant Physiology，2021，185（1）：161-178
[6] LORENZO C D，GARCÍA‐GAGLIARDI P，ANTONIETTI M S，et al. Improvement of alfalfa forage quality and management through the down‐regulation of MsFTa1[J]. Plant Biotechnology Journal，2020，18（4）：944-954
[7] PARK S J，JIANG K，TAL L，et al. Optimization of crop productivity in tomato using induced mutations in the florigen pathway[J]. Nature Genetics，2014，46（12）：1337-42
[8] RAJENDRAN S，HEO J，KIM Y J，et al. Optimization of tomato productivity using flowering time variants[J]. Agronomy，2021，11（2）：285
[9] ZHANG H，ZHU S，LIU T，et al. DELAYED HEADING DATE 1 interacts with Os HAP 5C/D， delays flowering time and enhances yield in rice[J]. Plant Biotechnology Journal，2019，17（2）：531-539
[10] PUTTERILL J，LAURIE R，MACKNIGHT R. It's time to flower： the genetic control of flowering time[J]. Bioessays，2004，26（4）：363-73
[11] AVRAHAM T，BADANI H，GALILI S，et al. Enhanced levels of methionine and cysteine in transgenic alfalfa （Medicago sativa L.） plants over-expressing the Arabidopsis cystathionine gamma-synthase gene. [J]. Plant Biotechnology Journal，2010，3（1）：71-79
[12] ZHOU Z，LI J，GAO Y，et al. Research on drought stress in Medicago sativa L. from 1998 to 2023： a bibliometric analysis[J]. Frontiers in Plant Science，2024，15：1406256
[13] SINGER S D，LEHMANN M，ZHANG Z，et al.Elucidation of physiological， transcriptomic and metabolomic salinity response mechanisms in Medicago sativa[J]. Plants，2023，12（10）：2059
[14] ABBASI A R，SARVESTANI R，MOHAMMADI B，et al. Drought stress-induced changes at physiological and biochemical levels in some common vetch（Vicia sativa L.） genotypes[J]. Journal of Agricultural Science and Technology，2014，16（3）：505-516
[15] YANG J，YI J，MA S，et al. Integrated physiological， metabolomic， and transcriptomic analyses elucidate the regulation mechanisms of lignin synthesis under osmotic stress in alfalfa leaf （Medicago sativa L.）[J]. BMC Genomics，2024，25（1）：174
[16] LI F F，LIU Y，XU F. The mystery of seed germination： an integrated physiological，ecological and molecular analysis[J]. Chinese Bulletin of Life Sciences，2024，36（12）：1470-1477 李飞飞，刘杨，徐飞. 种子萌发的奥秘：生理、生态与分子的综合解析[J]. 生命科学，2024，36（12）：1470-1477
[17] WOLABU T W，MAHMOOD K，JEREZ I T，et al. Multiplex CRISPR/Cas9‐mediated mutagenesis of alfalfa Flowering locus Ta1 （MsFTa1） leads to delayed flowering time with improved forage biomass yield and quality[J]. Plant Biotechnology Journal，2023，21（7）：1383-1392
[18] JOHANSSON M，STAIGER D. Time to flower： interplay between photoperiod and the circadian clock[J]. Journal of experimental botany，2015，66（3）：719-730
[19] PROSSER C L. Physiological variation in animals[J]. Biological Reviews，1995，30（3），229-261
[20] BERNIER G，PÉRILLEUX C. A physiological overview of the genetics of flowering time control[J]. Plant Biotechnology Journal，2005，3（1）：3-16
[21] PATRA S，CHATTERJEE D，DUTTA R，et al. Abiotic and biotic factors regulate the timing of floral induction： a review[J]. Physiologia Plantarum，2024，176（1）：e14199
[22] LIU L，XUAN L，JIANG Y，et al. Regulation by FLOWERING LOCUS T and TERMINAL FLOWER 1 in flowering time and plant architecture[J]. Small Structures，2021，2（4）：2000125
[23] GALINDO-SOTOMONTE L，JOZEFKOWICZ C，GÓMEZ C，et al. CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa[J]. Plant Cell Reports，2023，42（5）：953-956
[24] WANG Z，YANG R，DEVISETTY U K，et al. The divergence of flowering time modulated by FT/TFL1 is independent to their interaction and binding activities[J]. Frontiers in Plant Science，2017，8：697
[25] LORENZO C D，GARCÍA-GAGLIARDI P，GOBBINI M L，et al. MsTFL1A delays flowering and regulates shoot architecture and root development in Medicago sativa[J]. Plant Reproduction，2024，37（2）：229-242
[26] ZHANG P，LIU H，MYSORE K S，et al. MtFDa is essential for flowering control and inflorescence development in Medicago truncatula[J]. Journal of Plant Physiology，2021，260：153412
[27] WANG W，WANG C，WANG Y，et al. The P-body component DECAPPING5 and the floral repressor SISTER OF FCA regulate FLOWERING LOCUS C transcription in Arabidopsis[J]. The Plant Cell，2023，35（9）：3303-3324
[28] MA L，LIU X，LIU W，et al. Characterization of squamosa-promoter binding protein-box family genes reveals the critical role of MsSPL20 in alfalfa flowering time regulation[J]. Frontiers in Plant Science，2022，12：775690
[29] WU Z N，WEI Z W. Floral morphology and development of alfalfa[J]. Acta Agrestia Sinica，2013，21（1）：159-166 武自念，魏臻武. 苜蓿花的形态特征及发育过程[J]. 草地学报，2013，21（1）：159-166
[30] CHEN F，NIU K，MA H. Analysis on morphological characteristics and identification of candidate genes during the flowering development of alfalfa[J]. Frontiers in Plant Science，2024，15：1426838
[31] NAN M，LI J，WANG C，et al. Comparative study on yield and quality of spring sowing oat at different mowing stages in northwest arid region[J]. Acta Agrestia Sinica，2023，31（6）：1878-1885 南铭，李晶，王昶，等. 甘肃中部春播燕麦不同刈割期产量和品质比较研究[J]. 草地学报，2023，31（6）：1878-1885
[32] LI F F，ZHANG F F，WANG X Z，et al. Effects of cutting date and crop growth stage on alfalfa silage quality[J]. Acta Prataculturae Sinica，2019，28（12）：137-148 李菲菲，张凡凡，王旭哲，等. 刈割茬次和生育期对苜蓿青贮品质的影响[J]. 草业学报，2019，28（12）：137-148
[33] ZHANG X N，SONG S H，LIN Y Y，et al. Effects of growth stage and variety on yield and quality of alfalfa[J]. Acta Agrestia Sinica，2016，24（3）：676-681 张晓娜，宋书红，林艳艳，等. 生育期和品种对紫花苜蓿产量及品质的影响[J]. 草地学报，2016，24（3）：676-681
[34] BALL D M，COLLINS M，LACEFIELD G D，et al. Understanding forage quality[J]. American Farm Bureau Federation Publication，2001，1（1）：1-15
[35] KALU B A ， FICK G W .Quantifying morphological development of alfalfa for studies of herbage quality[J].Crop Science， 1981， 21（2）：267-271
[36] GAO R M，FEYISSA B A，CROFT M，et al. Gene editing by CRISPR/Cas9 in the obligatory outcrossing Medicago sativa[J]. Planta，2018，247（4）：1043-1050
[37] LACEFIELD D G. Alfalfa quality： What is it What can we do about it and， Will it pay[C]//Proceedings， National Alfalfa Symposium，2004：13-15
[38] TADEGE M，CHEN F，MURRAY J，et al. Control of vegetative to reproductive phase transition improves biomass yield and simultaneously reduces lignin content in Medicago truncatula[J]. BioEnergy Research，2015，8：857-867
[39] RUAN Y L，PATRICK J W，BOUZAYEN M，et al. Molecular regulation of seed and fruit set[J]. Trends in Plant Science，2012，17（11）：656-665
[40] WANG Y，CHANTREAU M，SIBOUT R，et al. Plant cell wall lignification and monolignol metabolism[J]. Frontiers in Plant Science，2013，4：220
[41] CHEN X，WEI Z W，REN H L，et al. Effect of photoperiod and germplasm characters on early-flowering time of burr medic （Medicago polymorpha L.）[J]. Chinese Journal of Grassland，2016，38（2）：34-40 陈祥，魏臻武，任海龙，等. 光周期与种质特性对南苜蓿初花期的影响[J]. 中国草地学报，2016，38（2）：34-40
[42] JAUDAL M，WEN J，MYSORE K S，et al. Medicago PHYA promotes flowering， primary stem elongation and expression of flowering time genes in long days[J]. BMC Plant Biology，2020，20：1-16
[43] LU D Y，WANG X，JIANG X，et al. Cloning and functional analysis of flowering regulation gene MsCOL2 in alfalfa[J]. Acta Agrestia Sinica，2023，31（10）：2905-2915 卢栋宇，王雪，蒋旭，等. 紫花苜蓿花期调控基因MsCOL2的克隆及功能研究[J]. 草地学报，2023，31（10）：2905-2915
[44] LI Z F， ZHANG J.， WEI F， et al. Identification of circadian clock-related genes regulated by photoperiod in legume plants. Molecular Plant Breeding， 2017，15（1）：1-11 李宗飞，张洁，魏芳，等.光周期调控豆科植物生物钟的相关基因的鉴定[J].分子植物育种， 2017， 14（1）：1-11
[45] ZHANG L L，JIANG X，CUI J，LI Y J，KANG J M. Cloning and functional validation of the alfalfa flowering regulation gene MsCIB2[J]. Acta Agrestia Sinica， 2025， 33（2）： 335-350 张丽丽，蒋旭，崔婧，栗亚静，康俊梅. 紫花苜蓿开花调控基因MsCIB2的克隆及功能验证[J]. 草地学报，2025，33（2）：335-350
[46] LIU Y，LI X，LI K，et al. Multiple bHLH proteins form heterodimers to mediate CRY2-dependent regulation of flowering-time in Arabidopsis[J]. PLoS Genetics，2013，9（10）：e1003861
[47] WANG N， XIE W G. Advances in the molecular mechanisms of flowering in forage grasses[J]. Grassland Science， 2019， 36（3）： 835-848 王娜，谢文刚. 牧草开花的分子机理研究进展[J]. 草业科学，2019，36（3）：835-848
[48] YANG H X，CHANG F，YOU C J，et al. Whole-genome DNA methylation patterns and complex associations with gene structure and expression during flower development in Arabidopsis[J]. The Plant Journal，2015，81（2）：268-281
[49] XU B，WANG Y Z，XU A K，et al. Study of simulation model of alfalfa plants development based on growing degree days[J]. Pratacultural Science，2016，33（1）：93-100 徐博，王英哲，徐安凯，等. 基于生长度日的紫花苜蓿生育期预测模型[J]. 草业科学，2016，33（1）：93-100
[50] ZHANG W Q，WANG Y L，ZHU D F，et al. Effect of increasing night temperature on floret opening and grain setting of rice[J]. Chinese Journal of Agrometeorology，2019，40（3）：180-185 张文倩，王亚梁，朱德峰，等. 花期夜温升高对水稻颖花开放及籽粒结实的影响[J]. 中国农业气象，2019，40（3）：180-185
[51] PUTTERILL J，ZHANG L，YEOH C C，et al. FT genes and regulation of flowering in the legume Medicago truncatula[J]. Functional Plant Biology，2013，40（12）：1199-1207
[52] YANG H，CHANG F，YOU C，et al. Whole‐genome DNA methylation patterns and complex associations with gene structure and expression during flower development in Arabidopsis[J]. The Plant Journal，2015，81（2）：268-281
[53] CHEN L，LI X，LIU H，et al. Comprehensive analysis of epigenetic modifications in alfalfa under cadmium stress[J]. Journal of Hazardous Materials，2025，482：136545
[54] LIU Y，WANG J，LIU B，et al. Dynamic regulation of DNA methylation and histone modifications in response to abiotic stresses in plants[J]. Journal of Integrative Plant Biology，2022，64（12）：2252-2274
[55] YANG Z，YAN H，WANG J，et al. DNA hypermethylation promotes the flowering of orchardgrass during vernalization[J]. Plant Physiology，2022，190（2）：1490-1505
[56] HUANG L，FENG G，YAN H，et al. Genome assembly provides insights into the genome evolution and flowering regulation of orchardgrass[J]. Plant Biotechnology Journal，2020，18（2）：373-388
[57] CHENG J Z，ZHOU Y P，LV T X，et al. Research progress on the autonomous flowering time pathway in Arabidopsis[J]. Physiology and Molecular Biology of Plants，2017，23：477-485
[58] HUANG T P，LI M J，WANG R，et al. Progress in study of gibberellins biosynthesis and signaling transduction pathway[J]. Plant Physiology Journal，2015，51（8）：1241-1247 黄桃鹏，李媚娟，王睿，等. 赤霉素生物合成及信号转导途径研究进展[J]. 植物生理学报，2015，51（8）：1241-1247
[59] LIU C W， XU R C， BIAN X C， et al. Regulation of flowering， endogenous hormones， and gene expression in broad bean by low temperature and gibberellin[J]. Jiangsu Agricultural Sciences， 2024， 52（24）： 105-112 刘陈玮，徐仁超，卞晓春，等. 低温和赤霉素对蚕豆开花、内源激素、基因表达的调控[J]. 江苏农业科学，2024，52（24）105-112
[60] FUKAZAWA J，OHASHI Y，TAKAHASHI R，et al. DELLA degradation by gibberellin promotes flowering via GAF1-TPR-dependent repression of floral repressors in Arabidopsis[J]. The Plant Cell，2021，33（7）：2258-2272
[61] BAO S，HUA C，SHEN L，et al. New insights into gibberellin signaling in regulating flowering in Arabidopsis[J]. Journal of Integrative Plant Biology，2020，62（1）：118-131
[62] BLÁZQUEZ M A，GREEN R，NILSSON O，et al. Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter[J]. The Plant Cell，1998，10（5）：791-800
[63] LAURIE R E，DIWADKAR P，JAUDAL M，et al. The Medicago FLOWERING LOCUS T homolog， MtFTa1， is a key regulator of flowering time[J]. Plant Physiology，2011，156（4）：2207-2224
[64] YEOH C C，BALCEROWICZ M，ZHANG L，et al. Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago[J]. PLoS One，2013，8（1）：e53467
[65] WELLER J L，MACKNIGHT R C. Functional genomics and flowering time in Medicago truncatula： an overview[J]. Functional Genomics in Medicago truncatula： Methods and Protocols，2018：261-271
[66] SONG Y H，SMITH R W，TO B J，et al. FKF1 conveys timing information for CONSTANS stabilization in photoperiodic flowering[J]. Science，2012，336（6084）：1045-1049
[67] ZHAO H，XU D，TIAN T，et al. Molecular and functional dissection of EARLY-FLOWERING 3 （ELF3） and ELF4 in Arabidopsis[J]. Plant Science，2021，303：110786
[68] LIN K，ZHAO H，GAN S，et al. Arabidopsis ELF4-like proteins EFL1 and EFL3 influence flowering time[J]. Gene，2019，700：131-138
[69] FARRÉ E M，HARMER S L，HARMON F G，et al. Overlapping and distinct roles of PRR7 and PRR9 in the Arabidopsis circadian clock[J]. Current Biology，2005，15（1）：47-54
[70] YANG M，HAN X，YANG J，et al. The Arabidopsis circadian clock protein PRR5 interacts with and stimulates ABI5 to modulate abscisic acid signaling during seed germination[J]. The Plant Cell，2021，33（9）：3022-3041
[71] MAEDA A E，MATSUO H，MURANAKA T，et al. Cold-induced degradation of core clock proteins implements temperature compensation in the Arabidopsis circadian clock[J]. Science Advances，2024，10（39）：eadq0187
[72] POKHILKO A，FERNÁNDEZ A P，EDWARDS K D，et al. The clock gene circuit in Arabidopsis includes a repressilator with additional feedback loops[J]. Molecular Systems Biology，2012，8（1）：574
[73] PAIK I，CHEN F，NGOC PHAM V，et al. A phyB-PIF1-SPA1 kinase regulatory complex promotes photomorphogenesis in Arabidopsis[J]. Nature Communications，2019，10（1）：4216
[74] LAUBINGER S，HOECKER U.The SPA1‐like proteins SPA3 and SPA4 repress photomorphogenesis in the light[J]. The Plant Journal，2003，35（3）：373-385
[75] WONG A C S，HECHT V F G，PICARD K，et al. Isolation and functional analysis of CONSTANS-LIKE genes suggests that a central role for CONSTANS in flowering time control is not evolutionarily conserved in Medicago truncatula[J]. Frontiers in Plant Science，2014，5：486
[76] LUO W，LI Y，MA P，et al. Transcriptomic analysis of Medicago truncatula under long-day conditions[J]. Diversity，2023，15（9）：1020
[77] SONMEZ C，BÄURLE I，MAGUSIN A，et al. RNA 3' processing functions of Arabidopsis FCA and FPA limit intergenic transcription[J]. Proceedings of the National Academy of Sciences，2011，108（20）：8508-8513
[78] AUSÍN I，ALONSO-BLANCO C，JARILLO J A，et al. Regulation of flowering time by FVE， a retinoblastoma-associated protein[J]. Nature genetics，2004，36（2）：162-166
[79] LIM M H，KIM J，KIM Y S，et al. A new Arabidopsis gene， FLK， encodes an RNA binding protein with K homology motifs and regulates flowering time via FLOWERING LOCUS C[J]. The Plant Cell，2004，16（3）：731-740
[80] MARTIGNAGO D，BERNARDINI B，POLTICELLI F，al LIKEet. The four FAD-dependent histone demethylases of Arabidopsis are differently involved in the control of flowering time[J]. Frontiers in Plant Science，2019，10：669
[81] ASLAM M，FAKHER B，JAKADA B H，et al. SWR1 chromatin remodeling complex： a key transcriptional regulator in plants[J]. Cells，2019，8（12）：1621
[82] MICHAELS S D，BEZERRA I C，AMASINO R M. FRIGIDA-related genes are required for the winter-annual habit in Arabidopsis[J]. Proceedings of the National Academy of Sciences，2004，101（9）：3281-3285
[83] OBERMEYER S，STÖCKL R，SCHNEKENBURGER T，et al. Distinct role of subunits of the Arabidopsis RNA polymerase II elongation factor PAF1C in transcriptional reprogramming[J]. Frontiers in Plant Science，2022，13：974625
[84] GÓMEZ-ZAMBRANO Á，CREVILLÉN P，FRANCO-ZORRILLA J M，et al. Arabidopsis SWC4 binds DNA and recruits the SWR1 complex to modulate histone H2A. Z deposition at key regulatory genes[J]. Molecular Plant，2018，11（6）：815-832
[85] YUAN L，SONG X，ZHANG L，et al. The transcriptional repressors VAL1 and VAL2 recruit PRC2 for genome-wide Polycomb silencing in Arabidopsis[J]. Nucleic Acids Research，2021，49（1）：98-113
[86] MOLITOR A M，LATRASSE D，ZYTNICKI M，et al. The Arabidopsis hnRNP-Q protein LIF2 and the PRC1 subunit LHP1 function in concert to regulate the transcription of stress-responsive genes[J]. The Plant Cell，2016，28（9）：2197-2211
[87] BAILE F，GÓMEZ-ZAMBRANO Á，CALONJE M. Roles of Polycomb complexes in regulating gene expression and chromatin structure in plants[J]. Plant Communications，2022，3（1）：003
[88] HARTMANN U，HÖHMANN S，NETTESHEIM K，et al. Molecular cloning of SVP： a negative regulator of the floral transition in Arabidopsis[J]. The Plant Journal，2000，21（4）：351-360
[89] LIU S， HOU D，VASUPALLI N，et al. Overexpression of PvSVP1， an SVP-like gene of bamboo， causes early flowering and abnormal floral organs in Arabidopsis and rice： PvSVP1 regulates flowering time and floral organ development[J]. Acta Biochimica et Biophysica Sinica，2023，55（2）：237
[90] LIU S，VASUPALLI N，HOU D，et al. Ectopic expression of a bamboo SVP-like gene alters flowering time and floral organs in Arabidopsis thaliana[J]. Plant Cell， Tissue and Organ Culture （PCTOC），2022，150（3）：721-732
[91] LEE J H，YOO S J，PARK S H，et al. Role of SVP in the control of flowering time by ambient temperature in Arabidopsis[J]. Genes & Development，2007，21（4）：397-402
[92] GALLEGO‐GIRALDO C，HU J，URBEZ C，et al. Role of the gibberellin receptors GID 1 during fruit‐set in Arabidopsis[J]. The Plant Journal，2014，79（6）：1020-1032
[93] TYLER L，THOMAS S G，HU J，et al. DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis[J]. Plant physiology，2004，135（2）：1008-1019
[94] HOU X，HU W W，SHEN L，et al. Global identification of DELLA target genes during Arabidopsis flower development[J]. Plant Physiology，2008，147（3）：1126-1142
[95] YU H，ITO T，ZHAO Y，et al. Floral homeotic genes are targets of gibberellin signaling in flower development[J]. Proceedings of the National Academy of Sciences，2004，101（20）：7827-7832
[96] HECHT V，FOUCHER F，FERRÁNDIZ C，et al. Conservation of Arabidopsis flowering genes in model legumes[J]. Plant Physiology，2005，137（4）：1420-1434
[97] YE Q，ZHOU C，LIN H，et al. Medicago2035： Genomes， Functional Genomics and Molecular Breeding[J]. Molecular Plant，2018，69（20）：4867-4880
[98] FUDGE J B，LEE R H，LAURIE R E，et al. Medicago truncatula SOC1 genes are up-regulated by environmental cues that promote flowering[J]. Frontiers in Plant Science，2018，9：496
[99] JAUDAL M，ZHANG L，CHE C，et al. A SOC1-like gene MtSOC1 a promotes flowering and primary stem elongation in Medicago[J]. Journal of Experimental Botany，2018，69（20）：4867-4880
[100] POULET A，ZHAO M，PENG Y，et al. Gene-edited MtSOC1 triple mutant Medicago plants do not flower[J]. Frontiers in Plant Science，2024，15：1357924
[101] JAUDAL M，ZHANG L，CHE C，et al. Three Medicago MtFUL genes have distinct and overlapping expression patterns during vegetative and reproductive development and 35S： MtFULb accelerates flowering and causes a terminal flower phenotype in Arabidopsis[J]. Frontiers in Genetics，2015，6：50
[102] ZHANG P，WANG R，WANG X，et al. MtFULc controls inflorescence development by directly repressing MtTFL1 in Medicago truncatula[J]. Journal of Plant Physiology，2021，256：153329
[103] JAUDAL M，ZHANG L，CHE C，et al. MtVRN2 is a Polycomb VRN 2‐like gene which represses the transition to flowering in the model legume Medicago truncatula[J]. The Plant Journal，2016，86（2）：145-160
[104] JAUDAL M，MAYO‐SMITH M，POULET A，et al. MtING2 encodes an ING domain PHD finger protein which affects Medicago growth， flowering， global patterns of H3K4me3， and gene expression[J]. The Plant Journal，2022，112（4）：1029-1050
[105] ZHANG Y X，JIANG X， YU C X，et al. The functional analysis of high mobility group ms HMG-Y involved in flowering regulation in Medicago sativa L[J]. Scientia Agricultura Sinica，2022，55（16）：3082-3092 张云秀，蒋旭，尉春雪，等. 紫花苜蓿高迁移率族蛋白基因Ms HMG-Y调控花期的功能分析[J]. 中国农业科学，2022，55（16）：3082-3092
[106] ZECHMANN B，MÜLLER M. Subcellular compartmentation of glutathione in dicotyledonous plants[J]. Protoplasma，2010，246：15-24
[107] CHAO Y H，ZHANG T J，YANG Q C，et al. Expression of the alfalfa CCCH-type zinc finger protein gene MsZFN delays flowering time in transgenic Arabidopsis thaliana[J]. Plant Science，2014，215/216：92-99
[108] KANG J M， JIANG X， ZHANG L L， et al. A novel MsCAL gene from alfalfa and its application： CN118620918A[P]. 2024-09-10 康俊梅，蒋旭，张丽丽，等. 一种紫花苜蓿MsCAL基因、及其应用：CN118620918A[P]. 2024-09-10
[109] MA X Q， GE L Q， WENG Y Y， et al. Alfalfa gene MsCYP20-3B and its application. CN201911207060.X[P]. 2025-02-26 马西青，葛玲巧，翁银银，等. 紫花苜蓿基因MsCYP20-3B及应用.CN201911207060.X[P].2025-02-26
[110] ZECHMANN B，KOFFLER B E，RUSSELL S D. Glutathione synthesis is essential for pollen germination in vitro[J]. BMC Plant Biology，2011，11：54
[111] ZECHMANN B，LIOU L C，KOFFLER B E，et al. Subcellular distribution of glutathione and its dynamic changes under oxidative stress in the yeast Saccharomyces cerevisiae[J]. FEMS Yeast Research，2011，11（8）：631-642
[112] TRAVERSO J A，PULIDO A，RODRÍGUEZ-GARCÍA M I，et al. Thiol-based redox regulation in sexual plant reproduction： new insights and perspectives[J]. Frontiers in Plant Science，2013，4：465
[113] SCHIPPERS J H，FOYER C H，VAN DONGEN J T. Redox regulation in shoot growth，SAM maintenance and flowering[J]. Current Opinion in Plant Biology，2016，29：121-128
[114] TRAP-GENTIL M V，HÉBRARD C，LAFON-PLACETTE C，et al. Time course and amplitude of DNA methylation in the shoot apical meristem are critical points for bolting induction in sugar beet and bolting tolerance between genotypes[J]. Journal of Experimental Botany，2011，62（8）：2585-2597
[115] ADHIKARI L，MAKAJU S O，MISSAOUI A M. QTL mapping of flowering time and biomass yield in tetraploid alfalfa （Medicago sativa L.）[J]. BMC Plant Biology，2019，19（1）：359
[116] JIANG X Q，YANG T H，ZHANG F，et al. RAD-seq-based high-density linkage maps construction and quantitative trait loci mapping of flowering time trait in alfalfa （Medicago sativa L.）[J]. Frontiers in Plant Science，2022，13：899681
[117] HE F，ZHANG F，JIANG X Q，et al. A genome-wide association study coupled with a transcriptomic analysis reveals the genetic loci and candidate genes governing the flowering time in alfalfa （Medicago sativa L.）[J]. Frontiers in Plant Science，2022，13：913947
[118] CUI J，LI Y，LIU H，et al. Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time[J]. BMC Plant Biology，2025，25（1）：87
[119] LIU Z P，LIU W X，YANG Q C，et al. Progress and existing problems of forage breeding in China[J]. Bulletin of National Natural Science Foundation of China，2023，37（4）：528-536 刘志鹏，刘文献，杨青川，等. 我国牧草育种进展及存在问题[J]. 中国科学基金，2023，37（4）：528-536
[120] WANG Q Q，XIE J H，YU L Q，et al. Research progress and prospect of alfalfa breeding in China[J]. Journal of Grassland and Forage Science，2023（4）：1-7 王旗旗，解继红，于林清，等. 我国苜蓿育种研究进展及展望[J]. 草学，2023（4）：1-7
[121] SUN Q Z，YU Z，XU C C. The pratacultural achievements in last decade and pratacultural perspectives in next decade in China[J]. Pratacultural Science，2011，28（12）：2215-2220 孙启忠，玉柱，徐春城. 我国草业新世纪10年取得的成就和未来10年发展的重点[J]. 草业科学，2011，28（12）：2215-2220
[122] TAO Y，SUN Q Z，XU L J，et al. Trends and challenges of alfalfa industry in China[J]. Grassland and Prataculture，2022，34（1）：1-10 陶雅，孙启忠，徐丽君，等. 我国苜蓿产业发展态势与面临的挑战[J]. 草原与草业，2022，34（1）：1-10
[123] XIE H L，YANG Y P，DONG Y，et al. Analysis on international development trends of alfalfa[J]. Chinese Bulletin of Botany，2021，56（6）：740-750 谢华玲，杨艳萍，董瑜，等. 苜蓿国际发展态势分析[J]. 植物学报，2021，56（6）：740-750
[124] WANG R G，XU W P. Development characteristics and trend of alfalfa industry in China[J]. Journal of Agricultural Science and Technology，2021，23（12）：7-12 王瑞港，徐伟平. 我国苜蓿产业发展特征与趋势分析[J]. 中国农业科技导报，2021，23（12）：7-12
[125] WANG Q Q，XIE J H，YU L Q，et al. Research progress and prospect of alfalfa breeding in China[J]. Journal of Grassland and Forage Science，2023（4）：1-7 王旗旗，解继红，于林清，等. 我国苜蓿育种研究进展及展望[J]. 草学，2023（4）：1-7
[126] CHEN C J，BAO M F，WANG W H，et al. Current situation and prospects for drought-resistance breeding in Medicago sativa[J]. Acta Prataculturae Sinica，2025，34（3）：204-223 陈彩锦，包明芳，王文虎，等. 紫花苜蓿抗旱育种研究现状及展望[J]. 草业学报，2025，34（3）：204-223
[127] ZHANG H，WANG M L，LI X J，et al. The changes in the contribution of scientific and technological progress of China’s alfalfa industry during the 13^TH five year plan period and the prospect of the 14^TH Five-Year Plan period[J]. Chinese Journal of Agricultural Resources and Regional Planning，2023，44（7）：194-205 张浩，王明利，李旭君，等. “十三五”时期我国苜蓿产业科技进步贡献变动及“十四五”展望[J]. 中国农业资源与区划，2023，44（7）：194-205
[128] PAN X，GAO Y，LIU B，et al. Current situation and prospect of alfalfa industry[J]. Journal of Green Science and Technology，2017，19（13）：104-107 潘霞，高永，刘博，等. 苜蓿产业发展现状及前景展望[J]. 绿色科技，2017，19（13）：104-107
[129] GUO T，XUE B，BAI J，et al. Discussion of the present situation of China’s forage grass industry development： an example using alfalfa and oats[J]. Pratacultural Science，2019，36（5）：1466-1473 郭婷，薛彪，白娟，等. 刍议中国牧草产业发展现状——以苜蓿、燕麦为例[J]. 草业科学，2019，36（5）：1466-1473
[130] GAO C X. Genome engineering for crop improvement and future agriculture[J]. Cell，2021，184（6）：1621-1635

Research Advances and Breeding Prospects on Alfalfa Flowering Time Regulation

苜蓿花期调控研究进展及育种展望

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