Effects of Biochar and Nitrogen Co-Application on Vegetation and Soil Restoration in Bare-Land Artificial Restoration Grassland

doi:10.11733/j.issn.1007-0435.2026.03.027

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (3): 1041-1052.DOI: 10.11733/j.issn.1007-0435.2026.03.027

Effects of Biochar and Nitrogen Co-Application on Vegetation and Soil Restoration in Bare-Land Artificial Restoration Grassland

ZHENG Wen-xian¹, LI Shi-xiong^1,2,3, ZHAO Wen¹, SONG Jiang-qin¹, HUO Jiu-yan¹, SUI Qi-qi¹, XU Hai-feng¹, YIN Ya-li^1,2

1. Academy of Animal Science and Veterinary Medicine Qinghai University, Xining, Qinghai Province 810016, China;
2. Key Laboratory of Alpine Grassland Ecosystem in the Three-River-Source, Ministry of Education, Qinghai University, Xining, Qinghai Province 810016, China;
3. Qinghai Provincial Key Laboratory of Adaptive Management on Alpine Grassland, Xining, Qinghai Province 810016, China

Received:2025-04-17 Revised:2025-07-09 Published:2026-03-23

炭氮配施对黑土滩人工草地植被与土壤的修复研究

郑文贤¹, 李世雄^1,2,3, 赵文¹, 宋江琴¹, 火久艳¹, 随奇奇¹, 徐海峰¹, 尹亚丽^1,2

1. 青海大学畜牧兽医科学院, 青海西宁 810016;
2. 三江源区高寒草地生态教育部重点实验室(青海大学), 青海西宁 810016;
3. 青海省高寒草地适应性管理重点实验室, 青海西宁 810016

通讯作者: 尹亚丽，E-mail：yaliyin@163.com
作者简介:郑文贤（1997-），男，汉族，甘肃古浪人，博士研究生，主要从事草地生态与环境保护研究，E-mail：2874009886@qq.com；
基金资助:
国家自然科学基金项目（32260357）；青海省科技项目（2023-ZJ-925M）资助

Abstract

Abstract: To investigate the effects of biochar combined with nitrogen fertilizer on vegetation， soil physicochemical properties， and extracellular enzyme activities in artificially restored bare-land grasslands in the Qilian Mountains， a randomized block design was conducted in 2021. The treatments included a basal fertilizer （biochar 0.5 t·hm^-² + organic fertilizer 1.5 t·hm^-²） with nitrogen additions of 50， 100， 150， and 200 kg·hm^-² （CN₁， CN₂， CN₃， CN₄）， as well as CN₀ （basal fertilizer only）， CN₅ （200 kg·hm^-² nitrogen without basal fertilizer）， and CK （no fertilization）， totaling seven treatments. The results showed that biochar-nitrogen application significantly increased aboveground biomass （AB）， belowground biomass （BB）， and root/shoot ratio （R/S）（P<0.05）. Specifically， CN₃ significantly enhanced soil water content （SWC）， total nitrogen （TN）， soil organic carbon （SOC）， and nitrate nitrogen （NO₃^--N）， while CN₃ and CN₄ significantly promoted the activities of β-1，4-acetylglucosaminidase （NAG）， leucine aminopeptidase （LAP）， and cellobiose hydrolase （CBH）（P<0.05）. Piecewise structural equation modeling （SEM） indicated that biochar-nitrogen application improved vegetation performance and soil fertility by stimulating extracellular enzyme activities and nutrient supply. In conclusion， CN₃ （basal fertilizer + 150 kg·hm^-² nitrogen， C∶N≈12∶1） showed the optimal effect， providing a theoretical reference for the restoration of bare-land artificial grassland in alpine regions.

Key words: Biochar and nitrogen fertilizer, Bare-land artificial restoration grassland, Soil physicochemical properties, Vegetation biomass, Extracellular enzyme activity

摘要： 为探究祁连山区生物炭配施氮肥对黑土滩人工草地植被、土壤理化性质及胞外酶活性的影响，本研究于2021年采用随机区组设计开展试验。试验设基肥（生物炭0.5 t·hm^-2+有机肥1.5 t·hm^-2），并在此基础上配施不同梯度的氮肥，分别为50，100，150，200 kg·hm^-2（对应处理CN₁~CN₄），并设CN₀（基肥）、CN₅（氮肥200 kg·hm^-2）和CK（不施肥），共7个处理。结果表明，氮配施显著提高植被地上、地下生物量和根冠比（P<0.05）。CN₃处理显著提高土壤含水量、全氮、有机碳和硝态氮含量。CN₃和CN₄处理显著增加β-1，4-乙酰氨基葡萄糖苷酶、亮氨酸氨基肽酶和纤维素二糖水解酶活性（P<0.05）。Piecewise SEM（Structural equation modeling）模型分析结果表明，炭氮配施通过提高胞外酶活性、促进养分供应，改善植被生长状况和土壤肥力。综上，CN₃（基肥+氮肥150 kg·hm^-2，C∶N≈12∶1）对黑土滩人工草地植被与土壤的修复效果最佳，本研究可为黑土滩草地修复提供理论参考。

关键词: 生物炭与氮肥, 黑土滩人工草地, 土壤理化性质, 植被生物量, 胞外酶活性

CLC Number:

S812

ZHENG Wen-xian, LI Shi-xiong, ZHAO Wen, SONG Jiang-qin, HUO Jiu-yan, SUI Qi-qi, XU Hai-feng, YIN Ya-li. Effects of Biochar and Nitrogen Co-Application on Vegetation and Soil Restoration in Bare-Land Artificial Restoration Grassland[J]. Acta Agrestia Sinica, 2026, 34(3): 1041-1052.

郑文贤, 李世雄, 赵文, 宋江琴, 火久艳, 随奇奇, 徐海峰, 尹亚丽. 炭氮配施对黑土滩人工草地植被与土壤的修复研究[J]. 草地学报, 2026, 34(3): 1041-1052.

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Effects of Biochar and Nitrogen Co-Application on Vegetation and Soil Restoration in Bare-Land Artificial Restoration Grassland

炭氮配施对黑土滩人工草地植被与土壤的修复研究

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