Abstract: Larvae of Galeruca daurica are the major pest that severely damages the grasslands of Inner Mongolia. To explore the molecular mechanisms by which 20-hydroxyecdysone （20E） regulates the development of its larval， transcriptome sequencing was conducted on 3rd-instar larvae treated with 20E for 48 hours by using the Illumina HiSeq^TM 4000 platform. A total of 75 536 Unigenes were obtained， among which 40 161 were functionally annotated. Compared with the control group， a total of 500 differentially expressed genes （DEGs） were identified， including 258 upregulated and 242 downregulated ones. GO and KEGG analyses revealed that the DEGs were significantly enriched in terms/pathways such as ion transport， catalytic activity， longevity regulating pathway-multiple species， and FOXO signaling pathway. Furthermore， 11 ecdysone-related genes were screened out. The qRT-PCR verification results confirmed the reliability of the sequencing data. The results indicated that 20E may regulate the growth and development of G. daurica larvae by affecting multiple metabolic pathways. This study systematically analyzed the transcriptomic characteristics of G. daurica larvae in response to 20E regulation， providing a theoretical basis for clarifying its developmental mechanism and screening green control targets.

Key words: Galeruca daurica, Larvae, 20-hydroxyecdysone, Transcriptome analysis, Ecdysis-related genes, FOXO signaling pathway

摘要： 沙葱萤叶甲（Galeruca daurica）幼虫是严重危害内蒙古草原的主要害虫。为探究蜕皮激素（20-hydroxyecdysone，20E）调控其幼虫发育的分子机制，本研究采用Illumina Hiseq^TM 4000平台对20E处理48小时的3龄幼虫进行转录组测序。共获得75 536条Unigene，其中40 161条获得功能注释。与对照组相比，共获得500个差异表达基因（Differentially expressed genes，DEGs），其中258个上调，242个下调。GO与KEGG分析显示，差异基因显著富集于离子转运（Ion transport）、催化活性（Catalytic activity）、多物种长寿调控通路（Longevity regulating pathway-multiple species）及FOXO信号通路（FOXO signaling pathway）等。进一步筛选出11个蜕皮激素相关基因。qRT-PCR验证结果证实测序数据可靠。结果表明，20E可能通过影响多种代谢通路调控沙葱萤叶甲幼虫的生长发育。本研究首次系统解析了20E调控沙葱萤叶甲幼虫的转录组特征，为阐明其发育机制及绿色防控靶点筛选提供了理论依据。

关键词: 沙葱萤叶甲, 幼虫, 蜕皮激素, 转录组分析, 蜕皮相关基因, FOXO信号通路