Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress

doi:10.11733/j.issn.1007-0435.2026.06.005

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (6): 2012-2021.DOI: 10.11733/j.issn.1007-0435.2026.06.005

Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress

HE Cai¹, LIU Yuan², JIN Min¹, WU Yuan¹, WANG Su-zhen¹, HU Fang¹

1. Wuwei Academy of Forestry, Wuwei, Gansu Province 733000, China;
2. College of Forestry, Gansu Agricultural University, Lanzhou, Gansu Province 730000, China

Received:2025-08-28 Revised:2025-10-16 Published:2026-06-02

沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究

何彩¹, 刘源², 晋敏¹, 吴媛¹, 王素桢¹, 胡芳¹

1. 武威市林业科学研究院, 甘肃武威 733000;
2. 甘肃农业大学林学院, 甘肃兰州 730000

通讯作者: 何彩,E-mail:hcyldfcl@163.com
作者简介:何彩(1985-),女,汉族,甘肃武威人,博士,正高级工程师,主要从事荒漠化防治研究,E-mail:hcyldfcl@163.com
基金资助:
甘肃省科技计划项目（自然科学基金）（24JRRH007，25JRRH004）；武威市科技创新人才计划项目（WW25A01RYFL002）资助

Abstract

Abstract: MYB-related gene family plays important roles in plant growth， development， and stress response. This study analyzed the number， evolutionary relationships， gene structure， and physicochemical properties of MYB-related family genes in Atraphaxis bracteata by bioinformatics methods based on the full-length transcriptome data of A. bracteate. The qRT-PCR was used to analyze the temporal and spatial expression patterns of these genes in various tissues of A. bracteata under drought stress， and to analyze their response to drought stress. The results showed that a total of 55 MYB-related family members of A. bracteata were identified and divided into 5 subgroups. The encoded proteins were mostly hydrophilic and mainly located in the nucleus. Motif 2 was the characteristic motif， while Motif 4 had the highest conservation. The secondary and tertiary structures of their proteins were mainly irregularly coiled. Expression analysis showed that after 24 h of drought stress， gene expression levels were higher than those at 6 h and 0 h， and most of these genes were highly expressed in the leaves. These genes responded more strongly in roots and stems under severe drought， while they had higher expression levels in leaves under mild drought. In summary， the MYB-related gene family of A. bracteata exhibited distinct tissue-specific expression patterns under drought stress， laying a theoretical foundation for subsequent functional research and stress resistant breeding.

Key words: Atraphaxis bracteata, MYB-related, Drought stress, Expression pattern

摘要： MYB-related基因家族在植物生长发育及逆境胁迫中起关键作用。本研究基于沙木蓼（Atraphaxis bracteata）全长转录组数据，采用生物信息学方法鉴定了其MYB-related基因家族，分析了进化关系、基因结构、理化性质，并采用qRT-PCR分析了其在干旱胁迫下各组织的时空表达模式。结果显示，共鉴定出55个成员，分为5个亚组；编码蛋白多呈亲水性，主要定位于细胞核，Motif 2为特征基序，Motif 4保守性最高，蛋白质二、三级结构以无规则卷曲为主。表达分析表明，干旱胁迫24 h后基因表达量均高于6 h和0 h，多数基因在叶中表达较高，这些基因在根茎中对重度干旱响应更强，而在叶中则对轻度干旱更为敏感。综上，沙木蓼MYB-related基因家族在干旱胁迫中具有组织特异性表达模式，为今后功能研究与抗逆育种提供了理论依据。

关键词: 沙木蓼, MYB-related, 干旱胁迫, 表达模式

CLC Number:

HE Cai, LIU Yuan, JIN Min, WU Yuan, WANG Su-zhen, HU Fang. Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress[J]. Acta Agrestia Sinica, 2026, 34(6): 2012-2021.

何彩, 刘源, 晋敏, 吴媛, 王素桢, 胡芳. 沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究[J]. 草地学报, 2026, 34(6): 2012-2021.

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Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress

沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究

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