Cloning MsLAC17 Gene and Analysis of its Response to Abiotic Stress in Medicago sativa ‘Pianguan’

doi:10.11733/j.issn.1007-0435.2026.06.006

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (6): 2022-2031.DOI: 10.11733/j.issn.1007-0435.2026.06.006

Cloning MsLAC17 Gene and Analysis of its Response to Abiotic Stress in Medicago sativa ‘Pianguan’

DU Xin-wei¹, RONG Rui-wei¹, HUA Yi-wen¹, LIU Yi¹, TAO Jia-li^1,2, ZHOU Si-yi¹, XU Tao¹, CEN Hui-fang¹, ZHU Hui-sen¹

1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. Department of Mining Engineering, Shanxi Institute of Technology, Yangquan, Shanxi Province 045000, China

Received:2025-08-20 Revised:2025-10-15 Published:2026-06-02

偏关苜蓿MsLAC17基因克隆及其对非生物胁迫的响应分析

杜欣蔚¹, 戎睿伟¹, 华怡雯¹, 刘怡¹, 陶佳丽^1,2, 周思懿¹, 许涛¹, 岑慧芳¹, 朱慧森¹

1. 山西农业大学草业学院, 山西太谷 030801;
2. 山西工程技术学院矿业工程系, 山西阳泉 045000

通讯作者: 朱慧森,E-mail:zhuhuisen@126.com
作者简介:杜欣蔚(2000-),女,汉族,山东淄博人,硕士研究生,主要从事牧草种质资源与育种相关研究,E-mail:dxwxiamo@163.com
基金资助:
国家自然科学基金项目（32071872）；山西省中央引导地方科技发展资金项目（YDZJSX2022B006）；现代种业发展项目（sxzyk202501-7）；山西省重点研发计划课题（202102140601006-3）；山西省基础研究计划（202303021221101）；山西省来晋科研奖励（SXBYKY2022118）；优秀博士启动项目（2021BQ01，2023BQ03）；山西省研究生实践创新项目（2024SJ133）；山西省研究生学术创新项目（2025XS300）资助

Abstract

Abstract: Laccase （LAC）， as an important member of the ceruloplasmin protein family， plays a key role in the regulation of plant growth and development and the response to abiotic stress. In this study， the local superior germplasm of Medicago sativa ‘Pianguan’ in Shanxi Province was used as the experimental material. The MsLAC17 gene was cloned by PCR. And was analyzed by bioinformatics means and fluorescence quantitative PCR technology. The tissue specificity expression of MsLAC17 gene and its response under different abiotic stresses were systematically explored. The results showed that the length of the MsLAC17 gene is 1782 bp， encoding 593 amino acids. The relative molecular mass of this protein is 66.26 kDa， and its theoretical isoelectric point is 10.05. It is a hydrophilic protein mainly located in chloroplasts and mitochondria. The results of phylogenetic analysis indicated that the LAC17 protein of Medicago sativa ‘Pianguan’ is closely related to that of Medicago truncatula. Fluorescence quantitative PCR detection revealed that the expression level of the MsLAC17 gene was the highest in the stem， and responded to salt， drought and ABA treatment. It is speculated that this gene may play a role in regulating the response of Medicago sativa ‘Pianguan’ to abiotic stress. The results of this study provide an important basis for further clarifying the biological function of the MsLAC17 gene.

Key words: Medicago sativa ‘Pianguan’, Abiotic stress, MsLAC17, Expression pattern

摘要： 漆酶（Laccase，LAC）作为铜蓝氧化酶蛋白家族的重要成员，在植物生长发育调控及非生物胁迫响应过程中扮演着关键角色。本研究选取山西地方优良种质‘偏关’苜蓿（Medicago sativa ‘Pianguan’）作为试验材料，采用PCR技术克隆获得MsLAC17基因，通过生物信息学手段对该基因进行分析，并利用荧光定量PCR技术，系统探究了MsLAC17基因表达的组织特异性，及其在不同非生物胁迫下的响应情况。结果显示：MsLAC17基因全长1782 bp，编码593个氨基酸，该蛋白的相对分子质量为66.26 kDa，理论等电点为10.05，属于亲水性蛋白，主要位于叶绿体和线粒体中。系统进化分析结果表明，‘偏关’苜蓿LAC17蛋白与蒺藜苜蓿（Medicago truncatula）的LAC17蛋白亲缘关系较近。荧光定量PCR检测发现MsLAC17基因在‘偏关’苜蓿茎中表达量最高，且对盐、干旱和ABA处理均有响应，推测该基因可能在调控‘偏关’苜蓿应对非生物胁迫的过程中发挥作用，本研究结果为进一步明晰MsLAC17基因的生物学功能奠定理论基础。

关键词: ‘偏关’苜蓿, 非生物胁迫, MsLAC17, 表达模式

CLC Number:

S541.9

DU Xin-wei, RONG Rui-wei, HUA Yi-wen, LIU Yi, TAO Jia-li, ZHOU Si-yi, XU Tao, CEN Hui-fang, ZHU Hui-sen. Cloning MsLAC17 Gene and Analysis of its Response to Abiotic Stress in Medicago sativa ‘Pianguan’[J]. Acta Agrestia Sinica, 2026, 34(6): 2022-2031.

杜欣蔚, 戎睿伟, 华怡雯, 刘怡, 陶佳丽, 周思懿, 许涛, 岑慧芳, 朱慧森. 偏关苜蓿MsLAC17基因克隆及其对非生物胁迫的响应分析[J]. 草地学报, 2026, 34(6): 2022-2031.

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Cloning MsLAC17 Gene and Analysis of its Response to Abiotic Stress in Medicago sativa ‘Pianguan’

偏关苜蓿MsLAC17基因克隆及其对非生物胁迫的响应分析

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