Colonization of Plant Growth-Promoting Rhizobacteria and its Effects on the Growth of Stipa Purpurea Under Different Temperature Conditions

doi:10.11733/j.issn.1007-0435.2026.06.034

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (6): 2316-2328.DOI: 10.11733/j.issn.1007-0435.2026.06.034

Colonization of Plant Growth-Promoting Rhizobacteria and its Effects on the Growth of Stipa Purpurea Under Different Temperature Conditions

YI Guo-yun^1,2, CHENG Liang^1,2, LI Juan^1,2, WANG Xin¹

1. Academy of Agriculture and Forestry, Qinghai University, Xining, Qinghai Province 810016, China;
2. Qinghai Provincial Key Laboratory of Agricultural Integrated Pest Management, Xining, Qinghai Province 810016, China

Received:2025-07-18 Revised:2025-11-12 Published:2026-06-02

根际促生菌的定殖及其对不同温度下紫花针茅生长的影响

伊国云^1,2, 程亮^1,2, 李娟^1,2, 王信¹

1. 青海大学农林科学院, 青海西宁 810016;
2. 青海省农业有害生物综合治理重点实验室, 青海西宁 810016

通讯作者: 程亮,E-mail:liangcheng1979@163.com;李娟,E-mail:18293130625@163.com
作者简介:伊国云(1998-),男,藏族,青海西宁人,博士研究生,主要从事微生物方向研究,E-mail:3476098428@qq.com
基金资助:
青海省自然科学基金项目项目-青年（2024-ZJ-942）资助

Abstract

Abstract: To screen plant growth-promoting rhizobacteria （PGPR） that are capable of stably colonizing the rhizosphere of Stipa purpurea， four PGPR strains previously isolated from this alpine grass were evaluated. Rifampicin-resistant derivatives were generated to monitor root-zone colonization dynamics， and the effects of bacterial inoculation on seed germination， seedling morphology， and physiological indices were assessed under the culture of 15℃， 20℃， and 25℃. Serratia proteamaculans GD-1-1 and Paenarthrobacter nitroguajacolicus YD-2-4 established stable populations in the S. purpurea rhizosphere， and the colonization amounts were 1.22×10⁸ and 0.06×10⁸ CFU·g^-1 at 20 d， respectively. Dilutions of YD-2-4 （1×10¹ and 1×10² CFU·mL^-1） significantly enhanced germination and seedling growth across 15℃-25℃， concurrently increasing antioxidant enzyme activities and lowering malondialdehyde content. In contrast， the stimulatory effect of GD-1-1 （1×10² and 1×10³ CFU·mL^-1） declined with temperature and disappeared at 15℃. These results indicated that YD-2-4 possessed robust colonization ability and broad temperature adaptability， making it an excellent candidate for the development of microbial inoculants for alpine grasslands.

Key words: Alpine grassland, Stipa purpurea, Plant growth-promoting rhizobacteria, Rifampicin resistance selection, Growth-promoting effect

摘要： 为筛选能有效定殖于紫花针茅根际的促生菌（PGPR），本研究以4株分离自紫花针茅根际的PGPR为候选菌株，通过利福平抗性筛选检测其根际定殖动态，并在15℃，20℃和25℃培养条件下，测定接种PGPR对紫花针茅发芽率、形态及生理指标的影响。结果显示，变形斑沙雷氏菌（Serratia proteamaculans）GD-1-1和硝基愈创木酚类节杆菌（Paenarthrobacter nitroguajacolicus）YD-2-4能有效定殖于紫花针茅根际，20 d时的定殖量分别为1.22×10⁸和0.6×10⁷ CFU·g^-1。菌株YD-2-4的1×10¹和1×10² CFU·mL^-1稀释液，在15℃~25℃范围内均能显著促进种子发芽和幼苗生长，同时提高植株抗氧化酶活性、降低丙二醛含量；而菌株GD-1-1的1×10²和1×10³ CFU·mL^-1稀释液的促进作用随温度降低而减弱，15℃下无显著促进效果。说明菌株YD-2-4根际定殖能力稳定且温度适应范围广，可作为高寒草地微生物菌剂开发的优良菌株。

关键词: 高寒草地, 紫花针茅, 植物根际促生菌, 利福平抗性筛选, 促生作用

CLC Number:

S543+.9

YI Guo-yun, CHENG Liang, LI Juan, WANG Xin. Colonization of Plant Growth-Promoting Rhizobacteria and its Effects on the Growth of Stipa Purpurea Under Different Temperature Conditions[J]. Acta Agrestia Sinica, 2026, 34(6): 2316-2328.

伊国云, 程亮, 李娟, 王信. 根际促生菌的定殖及其对不同温度下紫花针茅生长的影响[J]. 草地学报, 2026, 34(6): 2316-2328.

References

[1] DONG S K，TANG L，ZHANG X F，et al. Relationship between plant species diversity and functional diversity in alpine grassland[J]. Acta Ecologica Sinica，2017（5）：1472-1483 董世魁，汤琳，张相锋，等. 高寒草地植物物种多样性与功能多样性的关系[J]. 生态学报，2017（5）：1472-1483
[2] ZHANG J T. Exploring the influence of vegetation characteristics and environmental factors in typical alpine degraded grasslands on the Qing-Zang plateau[D]. Linfen：Shanxi Normal University，2022：2-7 张锦涛. 青藏高原典型高寒退化草地植被特征与环境因子的影响探究[D]. 临汾：山西师范大学，2022：2-7
[3] LI M，WANG J B，ZHANG X J，et al. Distribution of potential suitable areas of dominant species in alpine grasslands and alpine meadows in the TP under future climate scenarios[J]. Acta Ecologica Sinica，2024，44（22）：10162-10177 李明，王军邦，张秀娟，等. 未来气候情景下青藏高原高寒草原与高寒草甸优势种潜在适宜区分布预测[J]. 生态学报，2024，44（22）：10162-10177
[4] LU D X， JIN H，YANG X Y，et al. Characterization of rhizosphere and endophytic fungal communities from roots of Stipa purpurea in alpine steppe around Qinghai Lake[J]. Canadian Journal of Microbiology，2016，62（8）：643-656
[5] HAROON U，MUNIS M F H，LIAQUAT F，et al. Biofilm formation and flocculation potential analysis of halotolerant Bacillus tequilensis and its inoculation in soil to mitigate salinity stress of chickpea[J]. Physiology and Molecular Biology of Plants，2023，29（2）：277-288
[6] QI Z Q，LI X H，WANG Y Z，et al. Colonizing of antagonistic bacterium B67 isolate on soil and rhizosphere of cucumber[J]. China Vegetables，2009（8）：63-66 祁之秋，李兴海，王英姿，等. 黄瓜枯萎病拮抗芽孢杆菌B67在土壤及黄瓜根际的定殖[J]. 中国蔬菜，2009（8）：63-66
[7] JIN Y，GAO J. Study on colonization of endophytic antagonistic strain JYg07 of Schisandra chinensis[J]. Chinese Traditional and Herbal Drugs，2015，46（21）：3242-3247 金岩，高洁. 五味子内生拮抗菌JYg07的定殖研究[J]. 中草药，2015，46（21）：3242-3247
[8] YANG X X，HUANG X Q，ZHANG L，et al. Colonization of GFP-labelled Bacillus amyloliquefaciens Bam22 in Brassica napus[J]. Chinese Agricultural Science Bulletin，2022，38（1）：125-130 杨潇湘，黄小琴，张蕾，等. 绿色荧光蛋白标记解淀粉芽孢杆菌Bam22在油菜体内的定殖[J]. 中国农学通报，2022，38（1）：125-130
[9] WANG Z G，LIU Z P，HU Y L，et al. Molecular marker and colonization of Plantibacter sp. WZW03[J]. Acta Agriculturae Zhejiangensis，2018，30（10）：1715-1721 王志刚，刘泽平，胡云龙，等. 植物杆菌属（Plantibacter）菌株WZW03的分子标记与定殖促生能力[J]. 浙江农业学报，2018，30（10）：1715-1721
[10] LI Y，LONG C M，JIANG B，et al. Colonization on the peanuts of two plant-growth promoting rhizobacteria strains and effects on the bacterial community structure of rhizosphere[J]. Biotechnology Bulletin，2022，38（9）：237-247 李颖，龙长梅，蒋标，等. 两株PGPR菌株的花生定殖及对根际细菌群落结构的影响[J]. 生物技术通报，2022，38（9）：237-247
[11] SONG L Y，GAO P，ZHANG H，et al. Isolation，identification and colonization of biocontrol bacteria ZB36 against bitter gourd Fusarium wilt[J]. Journal of Henan Agricultural University，2024，58（2）：228-238 宋露洋，高沛，张涵，等. 苦瓜枯萎病生防细菌ZB36的分离鉴定及其定殖特性研究[J]. 河南农业大学学报，2024，58（2）：228-238
[12] WANG J Q，WANG J J，WANG Q，et al. Colonization of double-resistance strain of marine bacterium L₁-9 and its biocontrol effect on Fusarium wilt of cucumber[J]. Biotechnology Bulletin，2016，32（6）：193-198 王军强，汪晶晶，王琦，等. 海洋细菌L₁-9双抗菌株的定殖能力及其对黄瓜枯萎病的防治作用[J]. 生物技术通报，2016，32（6）：193-198
[13] LI M Y. Characterization and plant growth-promoting mechanism of cold-adapted PGPB from dominant herbage in the alpine grassland of the Qilian mountains[D]. Lanzhou：Gansu Agricultural University，2022：12-30 李明源. 祁连山高寒草地优势牧草耐冷促生菌特性及促生机理研究[D]. 兰州：甘肃农业大学，2022：12-30
[14] LEI S Y，ZHANG Y，SU B B，et al. Effects of plant growth promoting rhizobacteria （PGPR） on three cultivated forages in alpine area[J]. Acta Agrestia Sinica，2025，33（7）：2388-2399 雷生妍，张英，苏贝贝，等. 植物根际促生菌（PGPR）对高寒区3种栽培饲草促生效果研究[J]. 草地学报，2025，33（7）：2388-2399
[15] AO Y，FENG J R，YANG C D. Function determination and identification of endophytic bacteria 263ZY2 from Stipa purpurea in Alpine Grassland[J]. Grassland and Turf，2023，43（6）：38-43 敖远，冯疆蓉，杨成德. 高寒草地紫花针茅拮抗内生细菌263ZY2的功能测定及其鉴定[J]. 草原与草坪，2023，43（6）：38-43
[16] YANG C D，CUI Y Z，FENG Z H，et al. Effects of endophytic Bacillus subtilis 265ZY4 on physiological and biochemical characteristics of Stipa purpurea under abiotic stress[J]. Acta Prataculturae Sinica，2019，28（6）：101-108 杨成德，崔月贞，冯中红，等. 内生枯草芽孢杆菌265ZY4对温度和紫外光胁迫下紫花针茅生化特征的影响[J]. 草业学报，2019，28（6）：101-108
[17] GÓMEZ E，ALONSO A，SÁNCHEZ J，et al. Application of biostimulant in seeds and soil on three chickpea varieties： impacts on germination，vegetative development，and bacterial facilitation of nitrogen and phosphorus[J]. Life，2024，14（1）：148
[18] SONG J Q，YIN Y L，ZHAO W，et al. Characteristics of spatial differentiation of soil microbial communities in degraded grassland on the “black soil beaches” of Qinghai Plateau[J]. Ecology and Environmental Sciences，2024，33（11）：1696-1707 宋江琴，尹亚丽，赵文，等. 青海高原黑土滩退化草地土壤微生物群落空间分异特征[J]. 生态环境学报，2024，33（11）：1696-1707
[19] YI G Y，LI J，WANG X，et al. Screening of plant growth-promoting rhizobacteria and their growth-promoting activities for Stipa purpurea in alpine grassland[J]. Journal of Applied Microbiology，2025，136（3）：lxaf041
[20] ZHAO Z X，LI D M，TAN S M，et al. Colonization characteristics of Bacillus sp. Ya-1 and its effects on rhizosphere bacterial diversity of pepper[J]. Journal of Southern Agriculture，2024，55（11）：3346-3357 赵志祥，李道敏，谭诗梦，等. 芽孢杆菌Ya-1的定殖特性及其对辣椒根际细菌多样性的影响[J]. 南方农业学报，2024，55（11）：3346-3357
[21] QIU J，BAI Y G，FU Y B，et al. Spatial variation in temperature thresholds during seed germination of remnant Festuca hallii populations across the Canadian prairie[J]. Environmental and Experimental Botany，2010，67（3）：479-486
[22] GUO K Y，ZHANG K，YANG L，et al. Growth-promoting characteristics and fermentation optimization of Halomonas sp. L6 in cotton under saline-alkali stress[J]. Acta Agriculturae Boreali-occidentalis Sinica，2025，34（4）：639-652 郭珂妤，张凯，杨柳，等. 盐单胞菌L6对盐碱胁迫下棉花促生特性及发酵优化研究[J]. 西北农业学报，2025，34（4）：639-652
[23] YU X. A study on phosphate-solubilizing bacteria of rhizosphere soil in walnut plant grown in Sichuan Province[D]. Yaan：Sichuan Agricultural University，2011：23-25 余旋. 四川核桃主产区根际解磷细菌研究[D]. 雅安：四川农业大学，2011：23-25
[24] AHMAD H A，FARHANA L，HAROON U，et al. Rhizospheric bacterial strain Pseudomonas putida and fungal strain Penicillium chrysogenum alleviate Fusarium wilt of tomato by improving key growth attributes[J]. European Journal of Plant Pathology，2025，172（1）：123-142
[25] AHMAD ANSARI F，AHMAD I，PICHTEL J，et al. Pantoea agglomerans FAP10： A novel biofilm-producing PGPR strain improves wheat growth and soil resilience under salinity stress[J]. Environmental and Experimental Botany，2024，222：105759
[26] ZHANG Y Q，LI J M，CUI B H，et al. Research progress on the mechanism and application of rhizosphere microorganisms to the growth，development，and stress resistance of tobacco[J]. Biological Disaster Science，2024，47（4）：500-511 张玉芹，李军民，崔丙慧，等. 根际微生物对烟草生长发育及抗逆影响机制与应用研究进展[J]. 生物灾害科学，2024，47（4）：500-511
[27] ZENG X L. Preliminary study on endophytic Herbaspirillum sp. ZXN111 colonization and growth-promoting activity on tea plant Camellia sinensis var. assamica[D]. Hefei：Anhui Agricultural University，2020：27-30 曾秀丽. 内生草螺菌ZXN111在阿萨姆种茶树中的定殖及促生作用初步研究[D]. 合肥：安徽农业大学，2020：27-30
[28] ZHAO T L，DENG X L，XIAO Q Z，et al. IAA priming improves the germination and seedling growth in cotton （Gossypium hirsutum L.） via regulating the endogenous phytohormones and enhancing the sucrose metabolism[J]. Industrial Crops and Products，2020，155：112788
[29] MILJAKOVIĆ D，MARINKOVIĆ J，TAMINDŽIĆ G，et al. Bio-priming of soybean with Bradyrhizobium japonicum and Bacillus megaterium： strategy to improve seed germination and the initial seedling growth[J]. Plants，2022，11（15）：1927
[30] REN Z H，YU Q，GAO S K，et al. Screening and promoting effects of plant growth-promoting bacteria from rhizosphere of Campeiostachys nutans Griseb[J]. Acta Agrestia Sinica，2024，32（6）：1693-1701 任智慧，于乔，高士孔，等. 垂穗披碱草根际促生菌的分离鉴定及促生作用研究[J]. 草地学报，2024，32（6）：1693-1701
[31] ZHAO S D，LI J H. The study on screening and characteristics of rhizosphere growth-promoting bacteria of Poa alpigena L[J]. Acta Agrestia Sinica，2021，29（9）：1885-1891 赵树栋，李建宏. 高原早熟禾根际促生菌分离筛选及特性研究[J]. 草地学报，2021，29（9）：1885-1891
[32] QIAO S W，SHAO C，ZHANG H，et al. Effect on the seed germination and seedling growth of Glycyrrhiza uralensis under different concentration NaCl stress[J/OL]. https：//link.cnki.net/doi/10.16720/j.cnki.tcyj.2024.239，2024-12-16/2025-11-12 乔思薇，邵财，张浩，等. 不同浓度NaCl胁迫对甘草种子萌发和幼苗生长的影响[J/OL]. https：//link.cnki.net/doi/10.16720/j.cnki.tcyj.2024.239，2024-12-16/2025-11-12
[33] SONG P，ZHAO B，SUN X X，et al. Effects of Bacillus subtilis HS5B5 on maize seed germination and seedling growth under NaCl stress conditions[J]. Agronomy，2023，13（7）：1874
[34] MAKHAYE G，AREMU A O，GERRANO A S，et al. Biopriming with seaweed extract and microbial-based commercial biostimulants influences seed germination of five Abelmoschus esculentus genotypes[J]. Plants，2021，10（7）：1327
[35] LI Y D，WANG H X，HU B G，et al. Research progress on plant growth promoting rhizobacteria to alleviate abiotic stress tolerance of horticultural crops[J]. Acta Horticulturae Sinica，2024，51（8）：1964-1976 李娅娣，王瀚祥，胡柏耿，等. 植物根际促生菌缓解园艺作物非生物胁迫研究进展[J]. 园艺学报，2024，51（8）：1964-1976
[36] XIA T Q，ZHU X W，LU A G. Interactions of wheat PGPR and its effects on wheat growth[J]. Journal of Henan Agricultural Sciences，2009，38（5）：53-55 夏铁骑，朱学文，吕爱国. 小麦根际细菌的互作效应及其对小麦生长的影响[J]. 河南农业科学，2009，38（5）：53-55
[37] ZHU Y X，ZHANG Y Y，WANG X Y，et al. Wolbachia modify host cell metabolite profiles in response to short-term temperature stress[J]. Environmental Microbiology Reports，2024，16（5）：e70013

Colonization of Plant Growth-Promoting Rhizobacteria and its Effects on the Growth of Stipa Purpurea Under Different Temperature Conditions

根际促生菌的定殖及其对不同温度下紫花针茅生长的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Xiao-xuan, LI Run-jie, CUI Zi-long, XIN Ji-lin, LIN Cheng-qing, KONG Xiao-yun, DANG Yi-le, MA Zhan-ming, ZHANG Ming-yang, ZHANG Yong-kun. Study on the Variation Characteristics of Glomalin-Related Soil Protein and Soil Organic Carbon at Different Succession Stages of Alpine Meadow [J]. Acta Agrestia Sinica, 2026, 34(5): 1787-1797.
[2]	ZHANG Hao, MA Fu-lin, LIU Xiao-wei, YANG Cong-ying, MUTALIFU Wu-bu-li, WANG Xiao-li, ZHANG Qiang, GUO Liang. Effects of Winter Warming and Clipping on Soil Organic Carbon Fractions and Carbon Pool Stability in Alpine Grasslands of the Tibet Plateau [J]. Acta Agrestia Sinica, 2026, 34(3): 940-948.
[3]	HE Feng-yuan, CHEN Meng-jiao, REN Fei, LIU Xin-yan, LI Xiao-feng, LI Lan-ping, LIANG De-fei, HAN Cheng-long. Effects of Nitrogen Addition on Nitrogen and Phosphorus Contents of Plant Functional Groups under Fencing and Grazing Management [J]. Acta Agrestia Sinica, 2026, 34(3): 960-972.
[4]	WANG Yan, PEI Xiang-jun, XIU Yu-xin, WEI Qi, LI Ke, LI Qiang, ZHANG Xiao-chao, LI Qi, ZHOU Yu. Effects of Organic Amendments on the Physicochemical Properties and Microbial Community Structure of Rehabilitated Alpine Grassland Soils in Mining Areas [J]. Acta Agrestia Sinica, 2026, 34(3): 1106-1117.
[5]	ZHANG Huan-huan, LI Lin-hao, CHEN De-wei, HE Qiu-xia, JIANG Jun-hong, YUAN Xin, SUN Fei-da. Characteristics of Soil Enzyme Activity and Enzyme Stoichiometric Ratio in Different Types of Alpine Grasslands in Northern Xizang [J]. Acta Agrestia Sinica, 2025, 33(8): 2482-2493.
[6]	YAO Jun-fei, WEI Guo-liang, WU Fa-liang, LI Xu-dong, Adihaze, SHI Zheng-chen, ZHANG Zhong-hua, MA Li, LI Shan, LI Hong-lin, LI Qiang-feng, WANG Zhen, ZHOU Hua-kun, ZHANG Qiang. Effects of Different Grazing Management Practices on the Contents of Soils and Microbiomass and Their Stoichiometric Characteristics in Alpine Grassland [J]. Acta Agrestia Sinica, 2025, 33(6): 1749-1755.
[7]	LYU Liang-yu, SHI Jian-jun, CAI Zong-cheng, BAO Shan-cun, GAO Pei, LI Fa-yi, FU Shou-quan, LIU Yong-lu. Effects of Manure Combined with Chemical Fertilizer on Forage Production and Soil Nutrients in Degraded Alpine Grassland [J]. Acta Agrestia Sinica, 2025, 33(6): 2023-2032.
[8]	JIN Xin, ZHOU Xue-li, ZHENG Kai-fu, WANG Ying-cheng, WANG Jun-bang, LU Guang-xin. Differences in Soil Bacterial Community Assembly between Alpine Meadows and Alpine Steppes in the Qilian Mountains [J]. Acta Agrestia Sinica, 2025, 33(3): 789-797.
[9]	LI Zhen-wei, MIAO Yu-jue, ZONG Ning. Effects of Grazing Exclusion on Plant Diversity and Ecosystem Multifunctionality of Alpine Grasslands in Northern Xizang [J]. Acta Agrestia Sinica, 2025, 33(2): 596-608.
[10]	SONG Xue-ao, LI Shi-xiong, ZHAO Wen, LIU Jing-jing, WANG Yan-long, XU Hai-feng, YIN Ya-li. The Impacts of Different Restoration Approaches on the Vegetation and Soil Fungal Community Characteristics in Degraded Alpine Grasslands [J]. Acta Agrestia Sinica, 2025, 33(12): 3958-3968.
[11]	LIU Hong-jin, HAO Jia-hui, XU Xian-li, LUO Chong-liang, XU Shi-xiao. Vegetation Community Characteristics and Carrying Capacity of Wild Herbivores of Typical Alpine Grassland in Hoh Xil [J]. Acta Agrestia Sinica, 2025, 33(11): 3505-3514.
[12]	DUO Shu-wei, HUANG Yu-xuan, MA Cong-yu, LIU Lin, ZHOU Ji-qiong, LI Xu-xu, HUANG Nan. Screening and Characterization of Plant Growth-Promoting Bacteria from Salix Cupularis Rhizosphere in Alpine Desertification Land of Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2025, 33(11): 3603-3609.
[13]	HUANG Yu-xuan, DUO Shu-wei, MA Cong-yu, ZHOU Ji-qiong. Screening and Preliminary Identification of Dominant Plant Growth-Promoting Rhizobacterial Species in Alpine Desertification [J]. Acta Agrestia Sinica, 2025, 33(1): 34-40.
[14]	LYU Wei-dong, DONG Quan-min, SUN Cai-cai, LIU Wen-ting, FENG Bin, LIU Yu-zhen, ZHANG Zhen-xiang, YANG Xiao-xia. Effects of Warm-Season Grazing by Yak and Tibetan Sheep on Different Fractions of Carbon and Nitrogen in Alpine Grassland of the Qinghai-Xizang Plateau [J]. Acta Agrestia Sinica, 2025, 33(1): 125-135.
[15]	LI Yao, WU Jing-jing, WU Zhong-yu, XIU Yue, WU Cheng-jing, GONG Jin-chao, ZHAO Jia-rui, LI Lin-lin, YU Shui-quan, SUN Fei-da, MA Zhou-wen, LIU Lin, ZHOU Ji-qiong, LI Hong-lin, BAI Yan-fu. Temperature Sensitivity of Soil Organic Carbon Mineralization in Alpine Sandy Grasslands During Natural Regeneration [J]. Acta Agrestia Sinica, 2024, 32(9): 2686-2694.