Effects of Cp2-EPS Seed Coating on Alfalfa Seedling Growth and Photosynthetic Physiology under Drought Stress

doi:10.11733/j.issn.1007-0435.2026.07.035

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (7): 2741-2752.DOI: 10.11733/j.issn.1007-0435.2026.07.035

Effects of Cp2-EPS Seed Coating on Alfalfa Seedling Growth and Photosynthetic Physiology under Drought Stress

JIN Xu^1,2, HUANG Rong^1,2, ZUO Yan-yan^1,2, LIU Xue-mei^1,2, LEI Jie^1,2, LAN Tian-ya^1,2, ZHANG Zhen-fen¹

1. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Key Laboratory of Grassland Ecosystem, Ministry of Education, Engineering Laboratory of Gansu Province, Lanzhou, Gansu Province 730070, China

Received:2025-11-27 Revised:2026-01-14 Published:2026-07-02

干旱胁迫下Cp2-EPS种子包衣对紫花苜蓿幼苗生长及光合生理的影响

金旭^1,2, 黄荣^1,2, 左妍妍^1,2, 刘雪梅^1,2, 雷杰^1,2, 兰添雅^1,2, 张振粉¹

1. 甘肃农业大学草业学院, 甘肃兰州 730070;
2. 草业生态系统教育部重点实验室, 甘肃兰州 730070

通讯作者: 张振粉，E-mail:zhangzf@gsau.edu.cn
作者简介:金旭（2001-），男，汉族，陕西宝鸡人，硕士研究生，主要从事牧草病理学方向研究，E-mail:79924172@qq.com
基金资助:
甘肃省陇原青年英才专项（LYYC-2025-03）；甘肃农业大学伏羲杰出人才培育计划（Gaufx-05J01）；甘肃省林业与草原局草原生态修复治理项目（LCJ20240172）；甘肃省教育厅青年博士入企入园项目（2025QB-051）和国家自然科学基金（32060396）共同资助

Abstract

Abstract: Bacterial extracellular polysaccharides （EPS） show great potential in enhancing plant stress resistance， but their mechanism of action on drought resistance in alfalfa （Medicago sativa） remains unclear. In this study， Alfalfa ‘Juneng 551’ was used as the experimental material， and seed coating technology was employed to investigate the drought resistance effect of Cp2-EPS from Erwinia persicina. Treatments included an uncoated control （CK）， a coating matrix control （T1）， and four Cp2-EPS concentration treatments （0.5， 1.0， 1.5， 2.0 g·L^-1， denoted as T2-T5）. Drought stress was applied after 21 days of seedling growth by maintaining soil relative water content at 40% for 14 days， and related physiological and growth indicators were measured. The results showed that Cp2-EPS coating significantly alleviated the growth inhibition caused by drought in alfalfa， with the 1.0 g·L^-1 （T3） treatment demonstrating the best effect. Compared with CK， the T3 treatment increased root length， shoot length， fresh weight， and dry weight by 17.87%， 17.87%， 74.53%， and 46.34%， respectively. Leaf photochemical quantum yield Y（II）， maximum photochemical efficiency of PSII， and total chlorophyll content were also significantly improved. In summary， Cp2-EPS seed coating can mitigate drought-induced oxidative damage by enhancing photosynthetic performance， and 1.0 g·L^-1 was identified as the optimal concentration. This study provides theoretical and technical references for developing eco-friendly drought-resistant coating agents and sustainable alfalfa production.

Key words: Alfalfa, Exopolysaccharides, Seed coating, Drought stress

摘要： 细菌胞外多糖（EPS）在提升植物抗逆性方面潜力显著，但其对紫花苜蓿抗旱性的影响机制尚不明确。本研究以紫花苜蓿‘巨能551’为材料，采用种子包衣技术，探究桃色欧文氏菌胞外多糖Cp2-EPS的抗旱效果。设置未包衣对照（CK）、包衣基质对照（T1）及4个Cp2-EPS浓度处理（0.5，1.0，1.5，2.0 g·L^-1，记为T2~T5），于苗期生长21d后进行持续14 d的干旱胁迫（土壤相对含水量40%），并测定相关生理生长指标。结果表明，Cp2-EPS包衣显著缓解了干旱对紫花苜蓿生长的抑制，其中1.0 g·L^-1（T3）效果最好。与CK相比，T3处理下根长、苗长、鲜重和干重分别增加17.87%，17.87%，74.53%和46.34%；叶片光化学量子产量Y（Ⅱ）、PSⅡ最大光化学效率和总叶绿素含量也显著提升。综上，Cp2-EPS种子包衣可通过增强光合性能减轻干旱氧化损伤，1.0 g·L^-1为最佳浓度。本研究为开发环保抗旱包衣剂及紫花苜蓿可持续生产提供了理论与技术参考。

关键词: 紫花苜蓿, 胞外多糖, 种子包衣, 干旱胁迫

CLC Number:

S963.22+3.3

JIN Xu, HUANG Rong, ZUO Yan-yan, LIU Xue-mei, LEI Jie, LAN Tian-ya, ZHANG Zhen-fen. Effects of Cp2-EPS Seed Coating on Alfalfa Seedling Growth and Photosynthetic Physiology under Drought Stress[J]. Acta Agrestia Sinica, 2026, 34(7): 2741-2752.

金旭, 黄荣, 左妍妍, 刘雪梅, 雷杰, 兰添雅, 张振粉. 干旱胁迫下Cp2-EPS种子包衣对紫花苜蓿幼苗生长及光合生理的影响[J]. 草地学报, 2026, 34(7): 2741-2752.

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Effects of Cp2-EPS Seed Coating on Alfalfa Seedling Growth and Photosynthetic Physiology under Drought Stress

干旱胁迫下Cp2-EPS种子包衣对紫花苜蓿幼苗生长及光合生理的影响

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