Multidimensional Analysis of Physiological Differences in Seeds of Acanthopanax senticosus from Different Origins

doi:10.11733/j.issn.1007-0435.2026.09.014

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (9): 3292-3304.DOI: 10.11733/j.issn.1007-0435.2026.09.014

Multidimensional Analysis of Physiological Differences in Seeds of Acanthopanax senticosus from Different Origins

LI Xin-yu¹, GAO Ya-nan², LU Shan¹, ZHENG Lu¹, LI Xin¹, ZHENG Chun-ying¹, LIU Yang¹

1. College of Life Sciences, Heilongjiang University; Engineering Research Center of Agricultural Microbial Technology, Ministry of Education; Key Laboratory of Cold Region Ecological Restoration and Resource Utilization of Heilongjiang Province; Key Laboratory of Microbiology in Colleges and Universities of Heilongjiang Province, Harbin, Heilongjiang Province 150080, China;
2. Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin,Heilongjiang Province 150080, China

Received:2025-12-08 Revised:2026-01-28 Published:2026-09-11

刺五加不同产地种子生理差异的多维度解析

李欣雨¹, 高雅楠², 卢姗¹, 郑璐¹, 李欣¹, 郑春英¹, 刘洋¹

1. 黑龙江大学生命科学学院, 农业微生物技术教育部工程研究中心, 黑龙江省寒地生态修复与资源利用重点实验室, 黑龙江省普通高校微生物重点实验室, 黑龙江哈尔滨 150080;
2. 中国科学院东北地理与农业生态研究所大豆分子设计育种重点实验室, 黑龙江哈尔滨 150080

通讯作者: 郑春英, E-mail: zhengchunying68@163.com;刘洋, E-mail: 2021103@hlju.edu.cn
作者简介:李欣雨(2000-),女,汉族,黑龙江绥化人,硕士研究生,主要从事食品和药品生物活性挖掘与开发研究,E-mail:lixinyu2434@163.com
基金资助:
省属高校基本科研业务费项目（2024-KYYWF-0084）；黑龙江省“双一流”学科协同创新成果项目（LJGXCG2025-P08）；黑龙江大学种业创新项目（No.20230202）资助

Abstract

Abstract: To elucidate the physiological basis underlying provenance-dependent variation in seed germination of Acanthopanax senticosus， seeds collected from four locations in Heilongjiang Province， China-Acheng District （AC）， Mulan County （ML）， Gaoleng Town of Fangzheng County （GL）， and Tongbei County （TB）-were examined for phenotypic traits and germination performance， together with endogenous hormone dynamics during sand stratification and metabolomic profiles. The results showed that seeds from GL exhibited significantly higher germination rates and greater hundred-seed weight than those from the other provenances. In terms of hormonal regulation， GL seeds displayed the highest brassinosteroid （BR） content and the lowest abscisic acid （ABA） content at the late stage of stratification. Metabolomic analysis further indicated that prior to stratification， GL seeds were characterized by an accumulation of carbohydrates accompanied by reduced levels of stress-associated metabolites， whereas after stratification they preferentially enriched metabolites related to energy supply and biosynthetic processes. Weighted gene co-expression network analysis （WGCNA） revealed that the MEturquoise and MEred modules were positively associated with hundred-seed weight before stratification， while the MEyellow module was correlated positively with germination rate after stratification， highlighting key metabolite regulators. Collectively， these findings suggested that the superior germination potential of GL seeds was attributable to favorable seed traits， a more balanced hormonal status， and metabolically efficient reprogramming. This study provides a theoretical foundation for optimizing sand stratification practices and improving artificial propagation efficiency.

Key words: Acanthopanax senticosus, Seed germination, Endogenous hormones, Metabolomics

摘要： 为阐明不同产地刺五加（Acanthopanax senticosus）种子萌发差异的生理机制，本研究以黑龙江省阿城区（AC）、木兰县（ML）、方正县高楞镇（GL）和通北县（TB）4个产地种子为材料，分析其表型性状、萌发率、沙藏过程中内源激素动态变化及代谢组特征。结果表明，GL产地种子萌发率和百粒重显著高于其他产地。激素水平上，GL种子沙藏末期油菜素内酯（Brassinosteroid，BR）含量最高、脱落酸（Abscisic acid，ABA）含量最低。代谢组学结果显示，GL种子沙藏前富集糖类并下调胁迫相关代谢物，沙藏后富集能量供应与物质合成相关代谢物。加权基因共表达网络分析（Weighted Gene Co-expression Network Analysis，WGCNA）表明，沙藏前MEturquoise和MEred模块与百粒重显著正相关；沙藏后，MEyellow模块与萌发率呈显著正相关，明确了关键调控代谢物。综上，GL产地种子凭借优良表型、激素平衡与高效代谢表现出更强萌发潜力。研究为优化沙藏技术与提高人工繁育效率提供理论依据。

关键词: 刺五加, 种子萌发, 内源激素, 代谢组学

CLC Number:

Q945.6+5

LI Xin-yu, GAO Ya-nan, LU Shan, ZHENG Lu, LI Xin, ZHENG Chun-ying, LIU Yang. Multidimensional Analysis of Physiological Differences in Seeds of Acanthopanax senticosus from Different Origins[J]. Acta Agrestia Sinica, 2026, 34(9): 3292-3304.

李欣雨, 高雅楠, 卢姗, 郑璐, 李欣, 郑春英, 刘洋. 刺五加不同产地种子生理差异的多维度解析[J]. 草地学报, 2026, 34(9): 3292-3304.

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Multidimensional Analysis of Physiological Differences in Seeds of Acanthopanax senticosus from Different Origins

刺五加不同产地种子生理差异的多维度解析

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