›› 2004, Vol. 12 ›› Issue (3): 199-203.DOI: 10.11733/j.issn.1007-0435.2004.03.007

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Study on the Efficient Systems for Regeneration and AFL2 Gene Transformation of Puna Chicory (Cichorium Intybus. L)

CHENG Lin-mei1, CAO Qiu-fen1, GAO Hong-wen2, HUANG Jing1, MENG Yu-pin1   

  1. 1. Shanxi Agri-Biotechnolgy Research Center, Taiyuan, ShanxiProvince, 030031, China;
    2. Research and Development Center for Grassland Science, ChinaAcademy of Agriculture Sciences, Beijing, 10081, China
  • Received:2003-09-10 Revised:2003-12-25 Online:2004-08-15 Published:2004-08-15

菊苣再生体系建立及转AFL2基因的研究

程林梅1, 曹秋芬1, 高洪文2, 黄静1, 孟玉平1   

  1. 1. 山西省农业生物技术研究中心, 太原, 030031;
    2. 中国农业科学院草业研究中心, 北京, 100081
  • 作者简介:程林梅(1954- ),女,山西人,副研究员,主要从事作物抗旱生理、生物技术方面的研究
  • 基金资助:
    国家"863"计划(2001AA241165);山西省留学回国人员资助项目(20011575)

Abstract: A research was conducted using leaves of Puna chicory as explants to establish a highly efficient Puna chicory regeneration system and its stable agrobacterium mediated transformation system. The result shows that the chicory regenerating frequency was increased from 48% to 100%, while the number of regenerating single leaf buds appearing as many as 12. After three days of culturing the chicory leaf seedlings with agrobacterium strain FIG 121 Hm, and two weeks in regenerate medium containing 20 mg/L kanmycin and 600 mg/L cephalosorine, the chicory leaf seedlings regenerated into transgenic buds, with the regenerating rate reaching 8.3%. The buds could develop in a medium of 30 mg/L kanamycin and reproduce successively, which is tentatively determined to be transgenic buds.

Key words: Biotechnology, Cichorium intybus L. cv.Puna, Plant regeneration, AFL2 gene, Genetic transformation

摘要: 以菊苣叶片为外植体,研究建立高效再生体系和农杆菌介导的稳定转化体系。结果表明:再生频率从48%提高至100%;单叶盘再生芽数达12个;试管苗叶片与农杆菌菌株PIG121Hm共培养3d,在附加卡那霉素20mg/L和头孢霉素600mg/L再生培养基上选择培养2周后,叶片再生出转化芽,绿芽率达8.6%,转化芽在附加30mg/L卡那霉素培养基中长大并继代增殖,初步鉴定是转化芽。

关键词: 生物技术, 菊苣, 再生, AFL2基因, 遗传转化

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