›› 2012, Vol. 20 ›› Issue (6): 1117-1122.DOI: 10.11733/j.issn.1007-0435.2012.06.022

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Protective Effects of Exogenous Nitric Oxide on Oxidative Damage of Maize Seedling Leaves under NaCl Stress

WANG Fang, WANG Han-ning   

  1. Department of Agronomy, Gansu Agricultural University, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Labortory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu Province 730070, China
  • Received:2012-06-26 Revised:2012-10-25 Online:2012-12-15 Published:2012-12-28

外源NO对NaCl胁迫下玉米幼苗氧化损伤的保护作用

王芳, 王汉宁   

  1. 甘肃农业大学农学院 甘肃省干旱生境作物学重点实验室 甘肃省作物遗传改良与种质创新重点实验室, 甘肃 兰州 730070
  • 通讯作者: 王汉宁
  • 作者简介:王芳(1980-),女,甘肃民勤人,博士研究生,讲师,主要从事种子生理及玉米分子育种方面的研究,E-mail:wangfang@gsau.edu.cn;
  • 基金资助:
    甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1229);973计划前期研究专项(2012CB722902);科技部成果转化项目(2010GB2G100484)资助

Abstract: In order to clarify the physiological mechanism of nitric oxide (NO) on relief maize seedling growth, the protective effects of exogenous nitric oxide donor (sodium nitroprusside, SNP) at different concentrations on maize seedling oxidative damage due to 150 mmol稬-1 NaCl stress were investigated. Result showed that SNP significantly increased the antioxidant activities of SOD, CAT and POD, increased the proline and chlorophyll contents of leaves, while significantly decreased the MDA contents of leaves under NaCl stress compared with control. SNP had dosage effect on alleviating the NaCl stress of maize seedlings. The SNP of 0.1 mmol稬-1significantly elevated plant salt tolerance and had the best alleviating effect on NaCl stress damage.

Key words: Maize seedling, Nitric oxide, NaCl stress, Oxidative damage

摘要: 试验以150 mmol·L-1 NaCl为胁迫条件,用浓度为0.01,0.05,0.1,0.5和1 mmol·L-1的外源一氧化氮(NO)供体SNP处理玉米(Zea mays L.)幼苗,研究外源NO对NaCl胁迫下玉米幼苗氧化损伤的影响,旨在探讨NO对NaCl胁迫下玉米生长缓解的生理机理。结果表明:外源NO可促进脯氨酸(Pro)积累,提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性,提高可溶性糖的含量,并能缓解叶绿素的降解和丙二醛(MDA)含量的升高。NO的这种作用存在明显的剂量效应,其中以0.1 mmol·L-1 SNP处理效果最为显著。

关键词: 玉米幼苗, 一氧化氮, NaCl胁迫, 氧化损伤

CLC Number: