Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (5): 1039-1047.DOI: 10.11733/j.issn.1007-0435.2015.05.021

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Genetic Relationship Analysis of Hybrid F1 Generation of Multifoliolate Alfalfa and Trifoliolate Alfalfa by SSR Markers

ZHU Ping-hua, WEI Zhen-wu, QIAO Zhi-hong, WU Zi-nian, LIU Guo-zhi   

  1. College of Animal Science & Technology, Institute of Forage and Grassland Science, Yangzhou University, Yangzhou, Jiangsu Province 225009, China
  • Received:2014-06-13 Revised:2015-04-02 Online:2015-10-15 Published:2015-12-01

多叶型和三叶型苜蓿杂交F1代遗传关系的SSR分析

朱平华, 魏臻武, 乔志宏, 武自念, 刘国志   

  1. 扬州大学动物科学与技术学院扬州大学草业科学研究所, 江苏 扬州 225009
  • 通讯作者: 魏臻武
  • 作者简介:朱平华(1989-),女,江苏如皋人,硕士研究生,研究方向为牧草种质资源评价与遗传育种,E-mail:zhupinghua66@aliyun.com
  • 基金资助:

    国家自然科学基金项目(30972136);江苏省科技支撑计划项目"高产优质秧草新品种选育及种子繁育技术研究"(BE2012340);江苏省普通高校研究生科研创新计划项目(CXLX12_0932)(CXLX13_923)资助

Abstract:

To elucidate the separation characteristics and the genetic characteristics of multifoliolate traits of F1 hybrid generations of trifoliolate alfalfa and muitifoliolate alfalfa, Huaiyin alfalfa and muitifoliolate alfalfa PL34HQ were used to hybridize to generate reciprocal crossing F1 generation plants. 180 plants of F1 generation were used to analyze agronomic and economic characters, genetic diversity and population structure. Results showed that:the group multifoliolate rate of F1 population was 91.11%, the maximum multifoliolate rate of the plants with multifoliolate trait was 93.33%, and the minimum was 1.64%, 39.8% was the average. Group multifoliolate rate of F1 population in spring and autumn was higher than summer and winter. The proportion of purple stems, green stem and purple-green stem was nearly 1:1:1, and the proportion of plant type separated rate was about 9:7:2. A total of 79 allelic variations were detected with SSR markers, and 4.16 to per primer, the average ratio of polymorphism was 61.58%. F1 population was divided into four groups by genetic structure analysis, and the results of genetic diversity of the cluster analysis showed that highest genetic diversity existed in Group 2, followed by Group 1 and Group 4. Genetic diversity levels of Group 3 was the lowest.

Key words: Alfalfa, Muitifoliolate, F1 traits, Segregation of character, Population structure

摘要:

为了分析苜蓿(Medicago sativa)多叶性状的遗传规律,以及多叶苜蓿和三叶苜蓿杂交F1代性状分离特性和群体结构特点,本试验以淮阴苜蓿和多叶苜蓿品系PL34HQ为亲本,人工杂交获得F1群体。对F1群体180株苜蓿材料进行农艺性状和经济性状的差异分析,并从分子水平上进行遗传多样性和群体结构的分析。结果表明,F1群体春季多叶率达到91.11%,单株多叶率最高为93.33%,最低1.64%,平均为39.8%,春秋季节的群体多叶率高于夏冬季节;F1群体中紫色茎、绿色茎和紫绿色茎的植株出现的比例近乎1:1:1,株型的分离比接近9:7:2。SSR标记共检测到79个等位变异,平均每对引物检测到4.16个,19对引物的平均多态性比率是61.58%。群体结构分析将F1分为4个类群,类群2的遗传多样性最高,其次是类群1和类群4,类群3的遗传多样性水平最低。

关键词: 苜蓿, 多叶, F1代, 性状分离, 群体结构

CLC Number: