Effect of Arbuscular Mycorrhiza Inoculation on Cold Resistance in Elymus nutans

doi:10.11733/j.issn.1007-0435.2016.05.012

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (5): 1009-1015.DOI: 10.11733/j.issn.1007-0435.2016.05.012

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Effect of Arbuscular Mycorrhiza Inoculation on Cold Resistance in Elymus nutans

XU Ya-mei¹, CHU Xi-tong², WU Ye², MIAO Yan-jun¹, XU Yue-fei², HU Tian-ming², XIE Guo-ping¹

1. Department of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi, Tibet 860000, China;
2. College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi Province 712100, China

Received:2015-07-07 Revised:2016-04-20 Online:2016-10-15 Published:2017-01-13

接种丛枝菌根真菌对低温胁迫下垂穗披碱草影响的研究

徐雅梅¹, 褚希彤², 吴叶², 苗彦军¹, 许岳飞², 呼天明², 谢国平¹

1. 西藏农牧学院动物科学学院, 西藏林芝 860000;
2. 西北农林科技大学动物科技学院, 陕西杨凌 712100

通讯作者: 褚希彤, 许岳飞
作者简介:徐雅梅(1971-),女,硕士,副教授,主要从事西藏牧草驯化栽培及植被恢复的教学与科研工作,E-mail:xuyamei666@126.com
基金资助:
西藏自治区科技重点项目（2015ZDKJZC01-8）；西藏大学农牧学院“雪域英才工程”支持项目（2015XYB03）；陕西省自然科学基础研究计划项目（2016JM3025）；国家自然科学基金项目（31402129）资助

Abstract

Abstract:

Cold stress is one of the most important abiotic stresses affecting plant growth and metabolism. Arbuscular mycorrhizal fungi (AMF) are widespread symbiotic fungi, They could promote plant growth and development, and increase the ability of plant stress resistance. Two cultivars of Elymus nutans (ZD and KM) were subjected to 5℃ cold stress for 5 d after inoculated with or without Glomus mosseae (Nicolson & Gerdemann) and cultivated for 45 days in the pots. Seedling performances were estimated by AMF inoculation rate, changes in plant morphological and physiological performances. The results were as follows:AMF inoculation rates were quite different between the two E. nutans cultivars. From 0 to 45 d the inoculation rate increased steadily for both cultivars, and 45 d having the highest inoculation rates. However, from 45 to 60 d the inoculation rate showed a decrease. Under cold stress, plant growth was inhibited in both cultivars, however, plant shoot and root dry weight, root scanned morphology indexes, the content of total chlorophyll and carotenoid, and activities of antioxident enzymes tended to be higher with AMF inoculation. Moreover, the results showed that inoculated with AMF decreased relative electrolyte leakage (REL), malondialdehyde (MDA) content, O₂^·- content and H₂O₂ content in leaves of two cultivars of E. nutans. Our results suggested that AMF inoculation increased the cold tolerance in 45-day-old seedlings of ZD and KM.

Key words: Cold, Arbuscular mycorrhizas, Elymus nutans, Antioxidative system

摘要：

低温是影响植物生长的主要限制因子。采用沙培法研究了接种丛枝菌根真菌（arbuscular mycorrhizal fungi，AMF）对2份不同海拔梯度下的购买自北京正道公司（ZD）和采集自西藏康马县（KM）垂穗披碱草（Elymus nutans）生长与生理特性的影响。结果表明：接种AMF的植株根系均受到不同程度的侵染，且侵染率随时间的变化呈先增加后减少的趋势。低温胁迫下，2种垂穗披碱草的生长均受到一定程度的抑制。然而，接种活性AMF能显著提高低温胁迫下ZD和KM的地上和地下生物量、根系形态结构指标、光合色素含量和抗氧化物酶类活性，且显著降低低温胁迫下植株相对电解质渗透率（REL）、丙二醛（MDA）含量、超氧阴离子（O₂^·-）含量和过氧化氢（H₂O₂）含量（P<0.05）。综上所述，接种活性AMF能够明显提高45 d垂穗披碱草ZD和KM幼苗对低温胁迫的耐受性。

关键词: 低温胁迫, 丛枝菌根真菌, 垂穗披碱草, 抗氧化系统

CLC Number:

S543.9

XU Ya-mei, CHU Xi-tong, WU Ye, MIAO Yan-jun, XU Yue-fei, HU Tian-ming, XIE Guo-ping. Effect of Arbuscular Mycorrhiza Inoculation on Cold Resistance in Elymus nutans[J]. Acta Agrestia Sinica, 2016, 24(5): 1009-1015.

徐雅梅, 褚希彤, 吴叶, 苗彦军, 许岳飞, 呼天明, 谢国平. 接种丛枝菌根真菌对低温胁迫下垂穗披碱草影响的研究[J]. 草地学报, 2016, 24(5): 1009-1015.

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Effect of Arbuscular Mycorrhiza Inoculation on Cold Resistance in Elymus nutans

接种丛枝菌根真菌对低温胁迫下垂穗披碱草影响的研究

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