The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature

doi:10.11733/j.issn.1007-0435.2024.07.010

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (7): 2089-2098.DOI: 10.11733/j.issn.1007-0435.2024.07.010

The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature

DONG Shuang-hui^1,2, JIA Ming², ZHANG Hui², TENG Ke², TENG Wen-jun², WEN Hai-feng², FAN Xi-feng², YUE Yue-sen², WU Ju-ying², DING Guo-chang¹

1. College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China;
2. Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Bejing 100097, China

Received:2024-03-06 Revised:2024-04-23 Published:2024-08-03

四倍体青绿苔草对低温的生理响应及耐寒性评价

董双慧^1,2, 贾明², 张辉², 滕珂², 滕文军², 温海峰², 范希峰², 岳跃森², 武菊英², 丁国昌¹

1. 福建农林大学风景园林与艺术学院, 福建福州 350002;
2. 北京市农林科学院草业花卉与景观生态研究所农业农村部华北都市农业重点实验室, 北京 100097

通讯作者: 丁国昌，E-mail:fjdgc@fafu.edu.cn;武菊英，E-mail:wujuying@grass-env.com
作者简介:董双慧(2000-)女，汉族，湖南衡阳人，硕士研究生，主要从事观赏草抗逆性研究，E-mail:dsh13787345808@163.com
基金资助:
北京市农林科学院科技创新专项(KJCX20230811，KJCX20230119)资助

Abstract

Abstract: Diploid and homotetraploid Carex breviculmis strains 4,8,and 14 were used as plant materials of naturally occurring low-temperature treatments in the field in order to investigate the cold tolerance of various tetraploid Carex breviculmis strains and to screen the cold-tolerant tetraploid Carex breviculmis strains.Physiological indices of Carex breviculmis leaves were measured in October—November 2023 for each treatment time period.Principal component analysis and the affiliation func-tion approach were also used to assess the cold hardiness of each strain of tetraploid Carex breviculmis.The findings demonstrated that:during low temperature stress,the soluble sugar content increased to varying degrees,while the relative water content and chlorophyll content of the leaves of diploid and tetraploid Carex breviculmis decreased to different degrees;malondialdehyde content,superoxide dismutase activity,catalase activity,and soluble proteins content,displayed varied trends with increasing duration of low temperature,and the tetraploid Carex breviculmis strains 4,8,and 14 had greater levels of cold hardiness than the diploid Carex breviculmis strains;Carex breviculmis strain 14 cold hardiness was the highest.The aforementioned findings offer a theoretical foundation for better understanding Carex breviculmis's cold tolerance mechanism and for screening te-traploid strains of the organism that are resistant to cold.

Key words: Carex breviculmis, Tetraploidy, Cold hardiness, Comprehensive evaluation

摘要： 为探究四倍体青绿苔草(Carex breviculmis)不同株系的耐寒性，筛选耐寒四倍体青绿苔草株系，本研究以二倍体和同源四倍体青绿苔草株系4、株系8、株系14为例，采用田间自然低温处理，对2023年10月—11月各处理时间段的苔草叶片生理指标进行测定，同时通过主成分分析和隶属函数法，对四倍体青绿苔草各株系耐寒性进行了评价。结果表明：在低温胁迫过程中，两种倍性青绿苔草的叶片相对含水量和叶绿素含量均呈不同程度的下降，而可溶性糖含量呈不同程度的上升，丙二醛含量、超氧化物歧化酶活性、过氧化氢酶活性和可溶性蛋白含量随低温时长的增加呈不同变化趋势，四倍体青绿苔草株系4、株系8、株系14耐寒性均强于二倍体青绿苔草，其中，四倍体青绿苔草株系14耐寒力最强。上述研究结果为了解青绿苔草耐寒机制及四倍体青绿苔草耐寒株系筛选提供理论依据。

关键词: 青绿苔草, 四倍体, 耐寒性, 综合评价

CLC Number:

Q945.78

DONG Shuang-hui, JIA Ming, ZHANG Hui, TENG Ke, TENG Wen-jun, WEN Hai-feng, FAN Xi-feng, YUE Yue-sen, WU Ju-ying, DING Guo-chang. The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature[J]. Acta Agrestia Sinica, 2024, 32(7): 2089-2098.

董双慧, 贾明, 张辉, 滕珂, 滕文军, 温海峰, 范希峰, 岳跃森, 武菊英, 丁国昌. 四倍体青绿苔草对低温的生理响应及耐寒性评价[J]. 草地学报, 2024, 32(7): 2089-2098.

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The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature

四倍体青绿苔草对低温的生理响应及耐寒性评价

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