The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature

doi:10.11733/j.issn.1007-0435.2024.07.010

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (7): 2089-2098.DOI: 10.11733/j.issn.1007-0435.2024.07.010

The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature

DONG Shuang-hui^1,2, JIA Ming², ZHANG Hui², TENG Ke², TENG Wen-jun², WEN Hai-feng², FAN Xi-feng², YUE Yue-sen², WU Ju-ying², DING Guo-chang¹

1. College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China;
2. Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Bejing 100097, China

Received:2024-03-06 Revised:2024-04-23 Published:2024-08-03

四倍体青绿苔草对低温的生理响应及耐寒性评价

董双慧^1,2, 贾明², 张辉², 滕珂², 滕文军², 温海峰², 范希峰², 岳跃森², 武菊英², 丁国昌¹

1. 福建农林大学风景园林与艺术学院, 福建福州 350002;
2. 北京市农林科学院草业花卉与景观生态研究所农业农村部华北都市农业重点实验室, 北京 100097

通讯作者: 丁国昌，E-mail:fjdgc@fafu.edu.cn;武菊英，E-mail:wujuying@grass-env.com
作者简介:董双慧(2000-)女，汉族，湖南衡阳人，硕士研究生，主要从事观赏草抗逆性研究，E-mail:dsh13787345808@163.com
基金资助:
北京市农林科学院科技创新专项(KJCX20230811，KJCX20230119)资助

Abstract

Abstract: Diploid and homotetraploid Carex breviculmis strains 4,8,and 14 were used as plant materials of naturally occurring low-temperature treatments in the field in order to investigate the cold tolerance of various tetraploid Carex breviculmis strains and to screen the cold-tolerant tetraploid Carex breviculmis strains.Physiological indices of Carex breviculmis leaves were measured in October—November 2023 for each treatment time period.Principal component analysis and the affiliation func-tion approach were also used to assess the cold hardiness of each strain of tetraploid Carex breviculmis.The findings demonstrated that:during low temperature stress,the soluble sugar content increased to varying degrees,while the relative water content and chlorophyll content of the leaves of diploid and tetraploid Carex breviculmis decreased to different degrees;malondialdehyde content,superoxide dismutase activity,catalase activity,and soluble proteins content,displayed varied trends with increasing duration of low temperature,and the tetraploid Carex breviculmis strains 4,8,and 14 had greater levels of cold hardiness than the diploid Carex breviculmis strains;Carex breviculmis strain 14 cold hardiness was the highest.The aforementioned findings offer a theoretical foundation for better understanding Carex breviculmis's cold tolerance mechanism and for screening te-traploid strains of the organism that are resistant to cold.

Key words: Carex breviculmis, Tetraploidy, Cold hardiness, Comprehensive evaluation

摘要： 为探究四倍体青绿苔草(Carex breviculmis)不同株系的耐寒性，筛选耐寒四倍体青绿苔草株系，本研究以二倍体和同源四倍体青绿苔草株系4、株系8、株系14为例，采用田间自然低温处理，对2023年10月—11月各处理时间段的苔草叶片生理指标进行测定，同时通过主成分分析和隶属函数法，对四倍体青绿苔草各株系耐寒性进行了评价。结果表明：在低温胁迫过程中，两种倍性青绿苔草的叶片相对含水量和叶绿素含量均呈不同程度的下降，而可溶性糖含量呈不同程度的上升，丙二醛含量、超氧化物歧化酶活性、过氧化氢酶活性和可溶性蛋白含量随低温时长的增加呈不同变化趋势，四倍体青绿苔草株系4、株系8、株系14耐寒性均强于二倍体青绿苔草，其中，四倍体青绿苔草株系14耐寒力最强。上述研究结果为了解青绿苔草耐寒机制及四倍体青绿苔草耐寒株系筛选提供理论依据。

关键词: 青绿苔草, 四倍体, 耐寒性, 综合评价

CLC Number:

Q945.78

DONG Shuang-hui, JIA Ming, ZHANG Hui, TENG Ke, TENG Wen-jun, WEN Hai-feng, FAN Xi-feng, YUE Yue-sen, WU Ju-ying, DING Guo-chang. The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature[J]. Acta Agrestia Sinica, 2024, 32(7): 2089-2098.

董双慧, 贾明, 张辉, 滕珂, 滕文军, 温海峰, 范希峰, 岳跃森, 武菊英, 丁国昌. 四倍体青绿苔草对低温的生理响应及耐寒性评价[J]. 草地学报, 2024, 32(7): 2089-2098.

0
/ Save 0 / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2024.07.010

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2024/V32/I7/2089

References

[1] 谢媛圆. 温度胁迫对鲜食玉米生长生理及产量的影响研究[D]. 武汉:华中农业大学,2023:2-7 XIE Y Y. Effects of temperature stress on growth physiology and yield of fresh maize[D]. Wuhan:Huazhong Agricultural University,2023:2-7
[2] SOUALIOU S,DUAN Y,LI X,et al. Crop production under cold stress:an understanding of plant responses,acclimation processes,and management strategies[J]. Plant Physiology and Biochemistry,2022,190(21):47-61
[3] TENG K,TENG W J,WEN H F,et al. PacBiosingle-moleculelong-read sequencing shed new light on the complexity of the Carex breviculmis transcriptome[J]. BMC Genomics,2019,20(1):158-164
[4] 刘凌云,范希峰,腾珂,等. 苔草属植物遗传多样性研究进展[J]. 草地学报,2021,29(6):1148-1157 LIU L Y,FAN X F,TENG K,et al. Advances in development of genetic diversity of Carex L.[J]. Acta Agrestia Sinica,2021,29(6):1148-1157
[5] 孙建华,张刚,郝爱等. 浅谈青绿苔草在园林绿地建植中的应用[J]. 园艺与种苗,2023,43(6):21-23 SUN J H,ZHANG G,HAO A,et al. Application of Carex breviculmis in garden greenbelt construction[J]. Horticulture&Seed,2023,43(6):21-23
[6] 豆豆,李红. 黄河源区退化草地褐鳞苔草种植技术及对土壤改良效果研究[J]. 甘肃农业大学学报,2022,57(5):171-180 DOU D,LI H. Study on cultivating technology and soil improvement effect of Carex brunnescen on degraded grassland in the source area of the Yellow River[J]. Journal of Gansu Agricultural University,2022,57(5):171-180
[7] 宁花. 山东苔草属种质资源遗传多样性分析及抗旱性研究[D]. 泰安:山东农业大学,2014:12-29 NING H. Studies on the genetic diversity and drought resistance of sedges (Carex spp.) in Shandong Province[D]. Taian:Shandong Agricultural University,2014:12-29
[8] 李慧,滕珂,岳跃森,等. 不同引发方法对青绿苔草种子发芽的影响[J]. 草业科学,2021,38(8):1537-1547 LI H,TENG K,YUE Y S,et al. Effects of different priming methods on seed germination of Carex leucochlora[J]. Pratacultural Science,2021,38(8):1537-1547
[9] 叶艳然,王文莉,郑成淑,等. 四种野生苔草属植物的耐寒性评价[J]. 应用生态学报,2017,28(1):89-95 YE Y R,WANG W L,ZHENG C S,et al. Evaluation of cold resistance of four wild Carex speices[J]. Chinese Journal of Applied Ecology,2017,28(1):89-95
[10] 萧运峰,孙发政,高洁. 野生草坪植物——青绿苔草的研究[J]. 四川草原,1995,16(2):29-32 XIAO Y F,SUN F Z,GAO J. Research on Carex leucochlora of wild lawn plants[J]. Journal of Grassland and Forage Science,1995,16(2):29-32
[11] 贾民隆,张雨,宋卓琴等. 自然寒冻条件下3种苔草的抗性评价[J]. 山西农业科学,2020,48(12):1930-1933 JIA M L,ZHANG Y,SONG Z Q,et al. Resistance evaluation of three kinds of Carex under natural freezing conditions[J]. Journal of Shanxi Agricultural Sciences,2020,48(12):1930-1933
[12] 任智新,史建楠,何佳星,等. 盐胁迫对青绿苔草生长及生理特性的影响[J]. 草地学报,2022,30(8):2045-2052 REN Z X,SHI J N,HE J X,et al. Effect of salt stress on growth and physiological characteristics of Carex leucochlora[J]. Acta Agrestia Sinica,2022,30(8):2045-2052
[13] 袁小环,刘潇红,武菊英,等. 亏缺灌溉对青绿苔草生理与生长的影响[J]. 西北林学院学报,2015,30(3):61-65 YUAN X H,LIU X H,WU J Y,et al. Effect of deficit irrigation on physiology and growth of Carex leucochlora[J]. Journal of Northwest Forestry University,2015,30(3):61-65
[14] JIANG S,TANG Y,FAN R,et al. Response of Carex breviculmis to phosphorus deficiency and drought stress[J]. Frontiers in Plant Science,2023,14(7):1203924
[15] 朱红菊,刘文革. 植物同源多倍体耐盐性研究进展[J]. 遗传,2018,40(4):315-326 ZHU H J,LIU W G. Progress on salt resistance in autopolyploid piants[J]. Hereditas,2018,40(4):315-326
[16] 贾明,董双慧,潮洛濛,等. '四季’青绿苔草(C.breviculmis 'Siji’)多倍体诱导及鉴定[J/OL]. 北方园艺.https://link.cnki.net/urlid/23.1247.S.20240328.1738.002 JIA M,DONG S H,CHAO L M,et al. Polyploid induction and identification of C.breviculmis'Siji’[J/OL]. Northern Horticulture.https://link.cnki.net/urlid/23.1247.S.20240328.1738.002
[17] 李合生. 现代植物生理学[M]. 北京:北京高等教育出版社,2001:134-182 LI H S. Modern plant physiology[M]. Beijing Higher Education Press,2001:134-182
[18] 邹琦. 植物生理生化实验指导[M]. 北京:中国农业出版社,2000:7 ZOU Q. Plant physiology and biochemistry experiment guidance[M]. Beijing:China Agriculture Press,2000:7
[19] 高俊山. 植物生理学实验指导[M]. 北京:中国农业大学出版社,2018:93-132 GAO J S. Experimental supervision of plant physiology[M]. Beijing:China Agricultural University Press,2018:93-132
[20] 付超,周雪玲,朱春林. 应用隶属函数法综合评价高酸苹果抗寒性及果实品质[J]. 北方园艺,2017,41(2):11-15 FU C,ZHOU X L,ZHU C L. Comprehensive evaluation of cold resistance and fruit quality of apple with high acidity by subordinate function[J]. Northern Horticulture,2017,41(2):11-15
[21] LI Y P,LI H B,LI Y Y,et al. Improving water-use efficiency by decreasing stomatal conductance and transpiration rateto maintain higher ear photosynthetic rate in drought-resistant wheat[J]. The Crop Journal,2017,5(3):231-239
[22] LONG J L,XING W,WANG Y G,et al. Comparative proteomic analysis on chloroplast proteins provides new insights into the effects of low temperature in sugar beet[J]. Botanical Studies,2022,63(1):1-16
[23] 卢少云,郭振飞. 草坪草逆境生理研究进展[J]. 草业学报,2003,14(4):7-13 LU S Y,GUO Z F. Physiological responses of turfgrass to abiotic stresses[J]. Acta Prataculturae Sinica,2003,14(4):7-13
[24] 王园园,叶志琴,刘容,等. 二倍体和四倍体杂交兰幼苗对低温胁迫的生理响应差异分析[J]. 植物资源与环境学报,2014,23(4):68-74 WANG Y Y,YE Z Q,LIU R,et al. Analysis on difference in physiological response of diploid and tetraploid of Cymbidium hybrid seedling to low temperature stress[J]. Journal of Plant Resources and Environment,2014,23(4):68-74
[25] 吴建慧,李雪,王玲. 2种委陵菜叶片结构和生理指标对干旱胁迫的生理响应[J]. 草地学报,2015,23(1):25-129 WU J H,LI X,WANG L. The physiological response of anatomical structures and physiological indexes of two potentilla species to drought stress[J]. Acta Agrestia Sinica,2015,23(1):25-129
[26] TSIKAS D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples:Analytical and biological challenges [J]. Analytical Biochemistry,2017,524(1):13-30
[27] MINAMI A,NAGAO M,IKEGAMI K. Cold acclimation in bryophytes:low-temperature-induced freezing tolerance in Physcomitrella patens is associated with increases in expression levels of stress-related genes but not with increase in level of endogenous abscisic acid[J]. Planta,2005,220:414-423
[28] 叶艳然,付德静,郑成淑,等. 泰山四种苔草属植物的耐寒性研究[C]//中国园艺学会观赏园艺专业委员会,国家花卉工程技术研究中心.中国观赏园艺研究进展2016.2016:8 YE Y R,FU D J,ZHENG C S,et al. Study on the cold tolerance of four species of Carex L. in Taishan[C]// Chinese Society of Horticulture Ornamental Horticulture Professional Committee,National Flower Engineering Technology Research Center. Research progress of ornamental Horticulture in China 2016.2016:8
[29] 王冠群,李丹青,张佳平,等. 德国鸢尾6个品种的耐寒性比较[J]. 园艺学报,2014,41(4):773-780 WANG G Q,LI D Q,ZHANG J P,et al. Comparison of cold tolerance within 6 cultivars of lris germanica[J]. Acta Horticulturae Sinica,2014,41(4):773-780
[30] 陈虎,何新华,李晓强,等. 低温胁迫对龙眼幼树抗寒性的影响[J]. 广西农业科学,2010,41(1):7-10 CHEN H,HE X H,LI X Q,et al. Effects of low-temperature stress on cold resistance of longan sapling[J]. Guangxi Agricultural Sciences,2010,41(1):7-10
[31] SAED-MOUCHESHI A,SOHRABI F,FASIHFAR E. Superoxide dismutase (SOD) as a selection criterion for triticale grain yield under drought stress:a comprehensive study on genomics and expression profiling,bioinformatics,heritability,and phenotypic variability[J]. BMC Plant Biology,2021,21(1):148
[32] CHEN H F,WANG T T,LIU H Y,et al. Melatonin application improves salt tolerance of Alfalfa(Medicago Sativa L.) by enhanceing antioxidant capacity[J]. Plants,2020,9(2):220
[33] SOLIMAN MH,ALAYAFI AAM,EL KELISH AA,et al. Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress,improving photosynthesis,antioxidants and expression of cold stress responsive genes[J]. Botanical Studies,2018,59(1):1-17
[34] 姜慧. 香橼对主要非生物胁迫的生理响应机理研究[D]. 南京:南京农业大学,2012:13-19 JIANG H. Study on physiological responses mechhanism of Citrus medica under major abiotic stress[D]. Nanjing:Nanjing Agricultural University,2012:13-19
[35] HNILICKOVA H,KRAUS K,VACHOVA P,et al. Salinity stress effects photosynthesis,malondialdehyde formation,and proline content in Portula caoleracea L.[J]. Plants(Basel),2021,10(5):845
[36] 王雅美,张蜀宁,郑于莉,等. 二、四倍体不结球白菜抗寒基因表达及生理生长特性[J]. 南京农业大学学报,2014,37(5):41-46 WANG Y M,ZHANG S N,ZHENG Y L,et al. Expression of cold-related genes and physiological characteristics of the diploid and tetraploid non-heading Chinese cabbage[J]. Journal of Nanjing Agricultural University,2014,37(5):41-46
[37] 赵喜亭,范子建,刘露颖,等. 五倍体和同源十倍体'怀白菊’农艺性状及耐寒性的差异分析[J]. 植物生理学报,2017,53(3):461-469 ZHAO X T,FAN Z J,LIU L Y,et al. Differential agronomic traits and cold tolerances in Chrysanthemum morifolium 'Huaibai’ between pentaploid and its corresponding autodecaploid[J]. Plant Physiology Journal,2017,53(3):461-469
[38] DING Y L,SHI Y T,YANG S H. Advances and challenges in uncovering cold tolerance regulatory mechanisms in plants[J]. The New Phytologist,2019,222(4):1690-1704
[39] GUY C L,HUBER J L,HUBER S C. Sucrose phosphate synthase and sucrose accumulation at low temperature[J]. Plant Physiolgy,1992,100(1):502-508
[40] PATRA N,HARIHARAN S,GAIN H,et al. Typical but delicate Ca⁺⁺ re:dissecting the essence of calcium signaling network as a robust response coordinator of versatile abiotic and biotic stimuli in plants[J]. Frontiers in Plant Science,2021,12(1):752246
[41] 赵通,赵军营,张德,等. 8个扁桃品种的生理解剖特性及其抗寒性比较研究[J]. 干旱区资源与环境,2024,38(6):82-190 ZHAO T,ZHAO J Y,ZHANG D,et al. Comparative study on anatomical and physiological resistance of 8 almond varieties[J]. Journal of Arid Land Resources and Environment,2024,38(6):82-190
[42] 苏小雨,高桐梅,张鹏钰,等. 基于主成分分析及隶属函数法对芝麻苗期耐热性综合评价[J]. 作物杂志,2023(4):52-59 SU X Y,GAO T M,ZHANG P Y,et al. Comprehensive evaluation of heat resistance of sesame seedlings based on principal component analysis and membership function method[J]. Crops,2023(4):52-59
[43] 刘兴禄,王红平,孙文泰,等. 5个砧木苹果枝条的抗寒性评价[J]. 果树学报,2021,38(8):1264-1274 LIU X L,WANG H P,SUN W T,et al. Cold resistance evaluation of the shoots of 5 apple rootstocks[J]. Journal of Fruit Science,2021,38(8):1264-1274
[44] VAINOL A,REPO T. Cold hardiness of diploid and corresponding autotetraploid rhododendrons[J]. The Journal of Hortscience and Biochemistry,1999,74(5):541-546

The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature

四倍体青绿苔草对低温的生理响应及耐寒性评价

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles 0

Metrics

Comments

[1]	LIU Chun-yang, SUN Quan-wen, ZHAO Yue-ping, ZHAO Zhi-hai, HAN Yu, ZHAI Qing-lin, LIU Jing, FAN Guang-yu, WU Shu-qin. Evaluation of the Nutritional Value of Five millets (Setaria italica Beauv.) Varieties Using Grey Relational Analysis and Membership Function Method [J]. Acta Agrestia Sinica, 2024, 32(5): 1522-1528.
[2]	LÜ Yan-zhen, NIE Yu-ting, ZHANG Zhao, CUI Kai-lun, YAN Hui-fang. Comprehensive Evaluation of Seed Storability of Alfalfa and Screening of Key Indicators [J]. Acta Agrestia Sinica, 2024, 32(4): 1131-1141.
[3]	ZHANG Rui, WANG Jing, XU Ya-ling, LIU Qiu-xu, LI Xiang-yan, NIE Gang, ZHU Yong-qun. Comprehensive Evaluation of Cadmium Tolerance of 20 Sorghum bicolor×S. Sudanense Germplasm Resources in Seedling Stage [J]. Acta Agrestia Sinica, 2024, 32(4): 1153-1161.
[4]	WU Yi-ming, CUI Hui-ting, ZHANG Kun, LI Yue, LI Ming-na, SUN Yan. Physiological,Biochemical Responses and Comprehensive Evaluation of Two Carex rigescens Varieties in Response to Gradient NaCl Stress [J]. Acta Agrestia Sinica, 2024, 32(3): 736-745.
[5]	DU Li-xia, LIAO Ming-wang, QIN Shi-li, ZHANG Qian, LIU Wei. Effects of nitrogen application rate and row spacing on seed yield and quality of Leymus secalinus [J]. Acta Agrestia Sinica, 2024, 32(3): 968-976.
[6]	HOU Rui-hong, DU Ke, HUANG Wei-ye, GAO Jia-he, LIU Ya-ling, SHI Feng-ling, ZHANG Zhi-qiang, TANG Fang. Evaluation on Drought Resistance of 10 Wild Medicago ruthenica L. Materials from Different Provenances at Germination Period [J]. Acta Agrestia Sinica, 2024, 32(2): 489-494.
[7]	WANG Ling-xiu, SHAO Shuai, LOU Ya-hua. The Effect of Heterochronic Germination on the Growth and Quality of Four Forage Species in the Eremopyrum [J]. Acta Agrestia Sinica, 2024, 32(2): 495-502.
[8]	YUE Li, SHAN Qi-mike, WANG Hui, Zaituniguli·Kuerban, MAO Hong-yan, LIU Min. Comprehensive Evaluation of Drought Resistance of Sorghum Germplasm Resources during Germination in Xinjiang [J]. Acta Agrestia Sinica, 2024, 32(2): 553-561.
[9]	GAO Shan, YAN Chen-ming, WAN Chang, REN Wei, GUO Xing-yu, YU Hong-zhu, WANG Zhi-feng. Comprehensive Evaluation of Germplasm Resources of Leymus Chinensis Based on Grey Correlation Method and DTOPSIS Method [J]. Acta Agrestia Sinica, 2024, 32(2): 599-609.
[10]	ZHANG Jing-long, SHEN Yi-xin, LIU Xin-bao, CHI Ying-jun. Comprehensive Evaluation of Low Phosphorus Tolerance and Screening of Representative Evaluation Indexes at Sorghum Seedling [J]. Acta Agrestia Sinica, 2024, 32(1): 179-187.
[11]	WANG Yi-bo, CHEN Yi-tong, LIU Xin-di, GUO Yong-zhen, SUN Juan, ZHANG Hui, LI Yuan-yuan, GUO Shan-li. Study on Adaptability of Forage Chenopodium quinoa in Saline-alkali Land of Yellow River Delta [J]. Acta Agrestia Sinica, 2023, 31(6): 1810-1817.
[12]	CHEN Chao-chao, LI He-yang, YAN Yu-xin, SONG Qing, HUANG You, REZIYA·Aili, ZHANG Tie-jun. Evaluation on the Resistance of 20 Germplasms of Medicago sativa to Verticillium Wilt [J]. Acta Agrestia Sinica, 2023, 31(5): 1425-1434.
[13]	LI Jing, NAN Ming, LIU Yan-ming, ZHANG Cheng-jun, REN Sheng-lan, BIAN Fang. Comprehensive Evaluation of the Yield,Quality and Feeding Performance on Different Oat Varieties [J]. Acta Agrestia Sinica, 2023, 31(4): 1089-1098.
[14]	ZHU Kun, LIU Hua-jun, FENG Cheng-long, LI Bo, LIU Xin-yu, MA Hao-ran. Comprehensive Evaluation on the Salt Tolerance of Seed Germination of Different Alfalfa Varieties under Salt Stress [J]. Acta Agrestia Sinica, 2023, 31(12): 3724-3733.
[15]	ZHANG Xiao-juan, CHEN Cai-jin, ZHANG Jiu-pan, ZENG Yan-xia, Bao Ming-fang, ZHANG Shang-pei, SHANG Ji-hong, SHA Xiao-di, WU Juan, ZHANG Guo-hui, GAO Ting, YANG Tian-hui, WANG Chuan, SHI Shang-li. Analysis and Comprehensive Evaluation of Agronomic and Yield Traits of 55 Alfalfa Varieties [J]. Acta Agrestia Sinica, 2023, 31(11): 3453-3461.