Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (3): 547-552.DOI: 10.11733/j.issn.1007-0435.2019.03.004

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Effect of 2,4-epibrassinolide on Antioxidant Defense and Osmotic Adjustment of Elymus nutans Under Low Temperature Stress

PUBU Zhuo-ma1,2, LUO Yi-lan1, GAO Jin-zhu1, SUN Peng-yue1, ZHAO Dong-hao1, MIAO Yan-jun3, FU Juan-juan1, HU Tian-ming1   

  1. 1. Department of Grassland Science, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi Province 712100, China;
    2. Tibet Academy of Agriculture and Animal Husbandry Sciences, Lasa, Tibet 850000, China;
    3. College of Plant Science, Agricultural and Animal Husbandry College of Tibet University, Linzhi, Tibet 860000, China
  • Received:2018-12-21 Revised:2019-05-20 Online:2019-06-15 Published:2019-07-19

2,4表-油菜素内酯对低温胁迫下西藏野生垂穗披碱草幼苗抗氧化保护和渗透调节的影响

普布卓玛1,2, 罗艺岚1, 高金柱1, 孙鹏越1, 赵东豪1, 苗彦军3, 付娟娟1, 呼天明1   

  1. 1. 西北农林科技大学草业与草原学院, 陕西 杨凌 712100;
    2. 西藏自治区农科院草业科学研究所, 西藏 拉萨 850000;
    3. 西藏农牧学院植物科学学院, 西藏 林芝 860000
  • 通讯作者: 付娟娟, 呼天明
  • 作者简介:普布卓玛(1991-),女,藏族,西藏拉萨人,硕士研究生,主要从事植物逆境生物学研究,E-mail:puzhuo050509@nwafu.edu.cn
  • 基金资助:
    西北农林科技大学科研启动经费项目(2452017195);陕西省自然科学基础研究计划(2018JQ3006);中国博士后科学基金项目(2018M631208);国家自然科学基金面上项目(31872411)资助

Abstract: This study was conducted to determine the influence of the exogenous EBR (24-Epibrassinolide) on the antioxidant system and osmolytes of Tibetan wild-type E. nutans seedlings under cold stress. The results showed that the biomass of E. nutans increased with the low concentration of EBR but decreased with high concentration,while the relative conductivity of leaves showed opposite trend. 1 μmol·L-1 EBR had the best cold tolerance. Compared with control,cold stress resulted in the significant increase of SOD,GR,and APX activity and the slight suppression of POD and CAT activity. the stressed plants further increased the CAT,APX,and GR activities after EBR treated,while SOD activity had no significant change. In addition,exogenous EBR caused a significant increase in AsA,GSH,and free proline concentrations under cold stress,whereas reduced the accumulation of malondialdehyde (MDA) and superoxide anion radical. Therefore,spraying EBR could significantly increase the activity of antioxidant system,the accumulation of proline and the scavenging ability of reactive oxygen species under cold stress,alleviate cold-induced oxidative damages,and then enhance the E. nutans cold tolerance.

Key words: 2,4-epibrassinolide, Low temperature stress, Elymus nutans, Antioxidant system, Osmotic adjustment substance

摘要: 本研究以西藏野生垂穗披碱草(Elymus nutans Griseb.)为试材,采用叶面喷施2,4-表油菜素内酯(Epibrassinolide,EBR)的方法,探讨EBR对低温胁迫下西藏野生垂穗披碱草幼苗抗氧化保护和渗透调节的影响。结果表明,喷施不同浓度EBR导致垂穗披碱草幼苗生物量随浓度增加而升高,但高浓度下又有降低的趋势,而叶片相对电导率则呈相反变化趋势,其中1 μmol·L-1的EBR处理抗寒效果最好。低温胁迫下,抗氧化酶(抗坏血酸过氧化物酶,谷胱甘肽还原酶,超氧化物歧化酶)活性显著高于CK,而过氧化氢酶和过氧化物酶活性低于CK,EBR处理后抗氧化酶活性进一步增加,同时叶面喷施EBR提高了低温胁迫下垂穗披碱草幼苗抗坏血酸、谷胱甘肽和游离脯氨酸含量,降低了丙二醛和超氧阴离子自由基的积累。因此,低温胁迫下喷施EBR能显著提高抗氧化系统活性和渗透调节物质脯氨酸的积累,增加活性氧的清除能力,减轻低温引起的膜脂过氧化,从而增强垂穗披碱草的耐寒性。

关键词: 2, 4表-油菜素内酯, 低温胁迫, 垂穗披碱草, 抗氧化系统, 渗透调节物质

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