Effects of Exogenous Glutathione on Antioxidant System of Poa pratensis L. under Powdery Mildew Stress

doi:10.11733/j.issn.1007-0435.2024.10.008

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (10): 3080-3090.DOI: 10.11733/j.issn.1007-0435.2024.10.008

Effects of Exogenous Glutathione on Antioxidant System of Poa pratensis L. under Powdery Mildew Stress

WU Fan, ZHAO Yu-min, ZHANG Yi-ning, XU Zhi-yu, GAO Peng, ZHAO Xiang, ZHU Hui-sen, LIANG Yin-ping

College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2024-05-28 Revised:2024-07-23 Published:2024-11-04

外源谷胱甘肽对白粉菌胁迫下草地早熟禾抗氧化系统的影响

吴凡, 赵宇民, 张艺凝, 许志宇, 高鹏, 赵祥, 朱慧森, 梁银萍

山西农业大学草业学院, 山西太谷 030801

通讯作者: 梁银萍,E-mail:liangyinping3@163.com
作者简介:吴凡(1999-)，女，蒙古族，内蒙古呼和浩特人，硕士研究生，主要从事牧草病害防治研究，E-mail:17836504181@163.com
基金资助:
山西农业大学博士科研启动经费(2020BQ74);山西省高等学校科技创新项目(2021L166);博士来晋工作奖励资金科研项目(SXBYKY2021023);山西省中央引导地方科技发展资金(YDZJSX2022B006);山西省重点研发计划(202102140601006)资助

Abstract

Abstract: The occurrence of powdery mildew in kentucky bluegrass (Poa pratensis L.) seriously affects its ornamental value and life span. Glutathione (GSH) as a key antioxidant in plants plays an important role in the defense of plants against pathogen. In order to explore the role of plant antioxidant system under the regulation of exogenous GSH in the resistance of P.pratensis to powdery mildew,the medium-resistant variety ‘Taihang’ and the highly susceptible variety ‘Explorer’ were used as materials in this study. By spraying 0.1 mmol·L^-1 and 1.6 mmol·L^-1 exogenous GSH,the antioxidant indexes of plants were measured at 3 days and 7 days after inoculation with powdery mildew. The results showed that the activity of superoxide dismutase (SOD) in P.pratensis was negatively correlated with the disease resistance,and the activity of peroxidase (POD) was positively correlated with the disease resistance after the application of 0.1 mmol·L^-1 GSH at the early stage of powdery mildew infection (P<0.05). The activities of glutathione peroxidase (GPX) and glutathione S-transferase (GST) were significantly increased (P<0.05),and the resistance of P.pratensis to powdery mildew was stronger under 0.1 mmol·L^-1 GSH treatment. The present study clarified the physiological and biochemical mechanism of exogenous GSH enhancing plant powdery mildew resistance by regulating the antioxidant system of P. pratensis，which provided a theoretical basis for the prevention and control of powdery mildew in P.pratensis.

Key words: Poa pratensis L., Powdery mildew, Glutathione, Antioxidant system

摘要： 草地早熟禾(Poa pratensis L.)白粉病的发生严重影响其观赏价值和使用年限。谷胱甘肽(Glutathione，GSH)作为植物体内关键的抗氧化剂在植物抵御病原菌侵染过程中起重要作用。为探讨外源GSH调控下植物抗氧化系统在草地早熟禾抗白粉病中的角色，本研究以草地早熟禾中抗品种‘太行’和极感品种‘探险家’为材料，通过喷施0.1 mmol·L^-1和1.6 mmol·L^-1外源GSH，测定接种白粉菌后3 d和7 d植物抗氧化系统相关指标。结果表明在白粉菌侵染早期施用0.1 mmol·L^-1 GSH后草地早熟禾的超氧化物歧化酶(Superoxide dismutase，SOD)活性与抗病性呈负相关关系，过氧化物酶(Peroxidase，POD)活性与抗病性呈正相关关系；谷胱甘肽过氧化物酶(Glutathione peroxidase，GPX)和谷胱甘肽巯基转移酶(Glutathione S-transferase，GST)活性显著提高(P<0.05)，0.1 mmol·L^-1 GSH处理下草地早熟禾抗病能力更强。本研究明确了外源GSH通过调控草地早熟禾抗氧化系统增强白粉病抗性的生理生化机制，为防治草地早熟禾白粉病提供了理论依据。

关键词: 草地早熟禾, 白粉病, 谷胱甘肽, 抗氧化系统

CLC Number:

S435.121.46

WU Fan, ZHAO Yu-min, ZHANG Yi-ning, XU Zhi-yu, GAO Peng, ZHAO Xiang, ZHU Hui-sen, LIANG Yin-ping. Effects of Exogenous Glutathione on Antioxidant System of Poa pratensis L. under Powdery Mildew Stress[J]. Acta Agrestia Sinica, 2024, 32(10): 3080-3090.

吴凡, 赵宇民, 张艺凝, 许志宇, 高鹏, 赵祥, 朱慧森, 梁银萍. 外源谷胱甘肽对白粉菌胁迫下草地早熟禾抗氧化系统的影响[J]. 草地学报, 2024, 32(10): 3080-3090.

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Effects of Exogenous Glutathione on Antioxidant System of Poa pratensis L. under Powdery Mildew Stress

外源谷胱甘肽对白粉菌胁迫下草地早熟禾抗氧化系统的影响

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