Screening of Phosphorus Efficiency Germplasm and Analysis of Root Morphology Responding to Phosphorus Deficiency in Stylosanthes guianensis

doi:10.11733/j.issn.1007-0435.2021.05.003

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (5): 876-883.DOI: 10.11733/j.issn.1007-0435.2021.05.003

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Screening of Phosphorus Efficiency Germplasm and Analysis of Root Morphology Responding to Phosphorus Deficiency in Stylosanthes guianensis

LIU Yun-xi^1,2, LUO Jia-jia², LEI Jian^1,2, LIU Guo-dao², LIU Pan-dao²

1. College of Tropical Crops Hainan University, Haikou, Hainan Province 570228, China;
2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan Province 571101, China

Received:2021-01-06 Revised:2021-02-24 Online:2021-05-15 Published:2021-06-02

柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析

刘允熙^1,2, 罗佳佳², 雷健^1,2, 刘国道², 刘攀道²

1. 海南大学热带作物学院, 海南海口, 570228;
2. 中国热带农业科学院热带作物品种资源研究所, 农业部华南作物基因资源与种质创制重点实验室, 海南海口, 571101

通讯作者: 刘国道,E-mail:Guodao_Liu@163.com;刘攀道,E-mail:liupandao2019@163.com
作者简介:刘允熙(1996-)，女，海南大学，硕士，主要从事草业科学方面研究，E-mail:17854265518@163.com
基金资助:
海南省科协青年科技英才学术创新计划项目（No.QCXM201901）；中国科协青年人才托举工程第五届项目（No.2019QNRC001）；国家牧草产业技术体系岗位科学家项目（No.CARS-34）资助

Abstract

Abstract: To screen out germplasm with high phosphorus (P) utilization efficiency and provide insight into mechanism of root adapting to P deficiency in stylo (Stylosanthes guianensis),P utilization efficiency evaluation was performed by hydroponic experiments of 31 genotypes stylo,the effect of P deficiency on the stylo roots was analyzed,and the expression patterns of six expansins (EXP) genes responding to phosphate (Pi) starvation were also investigated in this study. Results showed that P deficiency led to significant reduction in the dry weight and total P content of stylo. P efficiency analysis combining with root morphology analysis suggested that stylo TF291 was highly responsive to P deficiency,its root growth was enhanced under low P condition. On the contrary,stylo TF343 was poorly responsive to P deficiency,its root growth was decreased by Pi starvation. qRT-PCR analysis showed that,the expression of SgEXPB2 in the TF291 and TF343 stylo roots were 3.73-fold and 1.52-fold greater in low P (-P) treatment compared to normal P (+P) treatment (P<0.05),respectively. The relative expression level of SgEXLB1 was significantly up-regulated by 1.61-fold in TF291 stylo roots subjected to P deficiency. Taken together,TF291 stylo had high P utilization efficient and was responsive to P availability,and the up-regulated expression of SgEXPB2 and SgEXLB1 genes in roots might be involved in the adaptation of stylo to P deficiency.

Key words: Stylosanthes guianensis, Phosphorus deficiency, P efficiency, Root morphology, Expansin

摘要： 为筛选磷高效柱花草（Stylosanthes guianensis）种质并解析其根系适应低磷胁迫的机理，本试验水培了31份柱花草，开展了磷效率评价，分析了低磷胁迫对柱花草根系的影响，并检测了6个扩展蛋白基因（Expansins，EXP）响应低磷胁迫的表达模式。结果表明，低磷胁迫显著降低柱花草的干重和全磷含量。磷效率分析结合根系表型显示，TF291为磷高效高响应型，且低磷胁迫显著促进其根系生长，TF343为磷低效低响应型，且低磷条件下其根系生长显著受抑制。荧光定量PCR表明，低磷处理（-P）使SgEXPB2在TF291和TF343根系中的表达分别增加3.73倍和1.52倍（P<0.05），SgEXLB1在TF291根系中表达增加1.61倍（P<0.05）。综上所述，本研究筛选到磷高效高响应型柱花草种质为TF291，且根系中SgEXPB2和SgEXLB1基因上调表达，可能参与柱花草适应低磷胁迫。

关键词: 柱花草, 低磷胁迫, 磷效率, 根系形态, 扩展蛋白

CLC Number:

S541

LIU Yun-xi, LUO Jia-jia, LEI Jian, LIU Guo-dao, LIU Pan-dao. Screening of Phosphorus Efficiency Germplasm and Analysis of Root Morphology Responding to Phosphorus Deficiency in Stylosanthes guianensis[J]. Acta Agrestia Sinica, 2021, 29(5): 876-883.

刘允熙, 罗佳佳, 雷健, 刘国道, 刘攀道. 柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析[J]. 草地学报, 2021, 29(5): 876-883.

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Screening of Phosphorus Efficiency Germplasm and Analysis of Root Morphology Responding to Phosphorus Deficiency in Stylosanthes guianensis

柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析

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