Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (4): 956-967.DOI: 10.11733/j.issn.1007-0435.2020.04.012

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Evaluation on Drought-resistance of Stylosanthes guianensis and Identification for Drought-resistance Germplasms in Seedling Stage

LIU Xiao-hui1,2, WANG Zhong-li3, WANG Meng-ru3, HUAN Heng-fu2, LIU Yi-ming2, LIU Guo-dao2   

  1. 1. College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
    2. Tropical Crop Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture&Rural Affairs, Danzhou, Hainan Province 571737, China;
    3. College of Forestry, Hainan University, Danzhou, Hainan Province 571737, China
  • Received:2020-02-19 Revised:2020-04-25 Online:2020-08-15 Published:2020-07-28

圭亚那柱花草苗期抗旱性评价及抗旱种质鉴定

刘小慧1,2, 王重丽3, 王梦茹3, 郇恒福2, 刘一明2, 刘国道2   

  1. 1. 南京农业大学草业学院, 江苏 南京 210095;
    2. 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物 基因资源与种质创制重点实验室, 海南 儋州 571737;
    3. 海南大学林学院, 海南 儋州 571737
  • 通讯作者: 刘一明, 刘国道
  • 作者简介:刘小慧(1995-),女,硕士研究生,主要从事热带牧草抗旱机制研究,E-mail:3014255470@qq.com
  • 基金资助:
    海南省自然科学基金项目资助（318MS101）；海南省重点研发计划项目（ZDYF2019078）；中央级公益性科研院所基本科研业务费专项资金（1630032019024）；“一带一路”热带农作物种质资源联合调查与技术示范推广（BARTP-01-CSB-LYM）；科技部基础资源调查工作专项：中国南方草地牧草资源调查（2017FY100600）；海南省高新技术产业发展（知识产权）专项资金资助

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Abstract: This study was aimed to evaluate the drought resistance of 93 Stylosanthes guianensis Sw. Materials under the simulated drought stress in a hydroponic system with 15% Polyethylene glycol-6000 (PEG-6000). Withered leaf rate (WLR),relative chlorophyll content (SPAD),relative water content (RWC),photosynthetic rate (Pn),stomatal conductance (Gs) and evapotranspiration rate (Tr) were measured at 5 and 14 days of drought stress. Correlation analysis,principal and membership function were used to evaluate drought resistance and screen the drought resistant germplasms. The membership function showed that under 5 days of drought stress,71(CIAT10598),73(CIAT10347),72[Wangtingbiao(Qi)],74(Graham),32(TPRC2001-54),39(TPRC2001-26),33[GC1480(IRRI)] and 37(TPRC2001-14) had the weakest drought resistance,which is shown as the drought-sensitive materials;under 14 day of drought stress,91(TPRC2001-83),82(Nan02145),19(CIAT11362),59(Endeavour),67(Reyan No.2),80(CIAT87830),55(TPRC90033),45(TPRC L2),27(TPRC2001-68),5(TPRC2001-85),23(TPRC2001-20) and 50[TPRC90005(1)] showed the strongest drought resistance. Further verification under soil conditions showed that, 59(Endeavour) and 23(TPRC2001-20) were more resistant than other germplasms.This study could provide theoretical basis for drought breeding of Stylosanthes.

Key words: Stylosanthes guianensis Sw., Drought tolerance, DTI, Comprehensive evaluation

摘要： 本研究用水培试验方法，分别在15%聚乙二醇6000（Polyethylene glycol，PEG-6000）模拟干旱胁迫5 d和14 d后对93份圭亚那柱花草（Stylosanthes guianensis Sw.）的枯叶率、相对叶绿素含量、相对含水量、净光合速率、气孔导度及蒸腾速率进行测定，运用相关性分析、主成分分析、隶属函数法等方法对圭亚那柱花草种质进行抗旱性综合评价并筛选抗旱种质。隶属函数抗旱能力排序表明，在处理的第5 d，71（CIAT10598），73（CIAT10347），72[Wangtingbiao（Qi）]，74（Graham），32（TPRC2001-54），39（TPRC2001-26），33[GC1480（IRRI）]和37（TPRC2001-14）等8份材料抗旱性最差，表现为干旱敏感材料；在处理的第14 d，91（TPRC2001-83），82（Nan02145），19（CIAT11362），59（Endeavour），67（Reyan No.2），80（CIAT87830），55（TPRC90033），45（TPRC L2），27（TPRC2001-68），5（TPRC2001-85），23（TPRC2001-20）和50[TPRC90005（1）]等12份材料表现出较强抗旱能力。根据隶属函数排名选取部分材料进行土壤条件下抗旱性验证，结果表明59（Endeavour）和23（TPRC2001-20）的抗旱性明显优于其它材料。本试验可为柱花草抗旱育种提供理论依据。

关键词: 圭亚那柱花草, 抗旱, DTI, 综合评价

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