Metabolic Derivatives Analysis of Desmodium heterocarpon in Response to Phosphorus Deficiency Stress

doi:10.11733/j.issn.1007-0435.2022.06.013

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (6): 1422-1429.DOI: 10.11733/j.issn.1007-0435.2022.06.013

Metabolic Derivatives Analysis of Desmodium heterocarpon in Response to Phosphorus Deficiency Stress

ZHU Hou-rong^1,2, ZHENG Shao-jue^1,2, LYU Jin-hui^1,2, XU Ran-ran^1,2, LUO Li-juan¹, DONG Rong-shu²

1. College of Tropical Crops, Hainan University, Haikou, Hainan Province 570228, China;
2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan Province 571101, China

Received:2021-12-23 Revised:2022-03-16 Published:2022-07-05

假地豆响应缺磷胁迫的代谢衍生物分析

朱厚荣^1,2, 郑韶爵^1,2, 吕金慧^1,2, 徐然然^1,2, 罗丽娟¹, 董荣书²

1. 海南大学热带作物学院, 海南海口 570228;
2. 中国热带农业科学院热带作物品种资源研究所, 海南海口 571101

通讯作者: 罗丽娟,E-mail:luoljd@126.com;董荣书,E-mail:dongrongshu@126.com
作者简介:朱厚荣(1998-),男,海南东方人,硕士研究生,主要从事草业科学方面研究,E-mail:1872346064@qq.com
基金资助:
海南省重点研发计划科技合作方向项目（No.ZDYF2019194）；财政部和农业农村部国家现代农业产业技术体系（CARS-34，CARS-22）；滇桂黔石漠化地区特色作物产业发展关键技术集成示范（SMH2019-2021）资助

Abstract

Abstract: To investigate the physiological and metabolic responses of Desmodium heterocarpon to phosphorus(P) deficiency stress,the effects of normal P supply (+P treatment) and P deficiency (-P treatment) on the growth and metabolite accumulation of D.heterocarpon were analyzed by hydroponic experiments. The results showed that P deficiency stress decreased shoot dry weight and P content of D.heterocarpon,but promoted root growth. Subsequently,the metabolome detected a total of 267 metabolites in the leaves and roots of D.heterocarpon,which could be classified into 12 categories. Among them,the differential accumulation metabolites (DAMs) of P deficiency stress in both leaves and roots were 79. The P deficiency treatment resulted in a significant increase in the accumulation of proanthocyanidins,quercetin,and their derivatives,and a significant decrease in the accumulation of 2'-deoxyadenosine 5'-monophosphate (dAMP) in the leaves and roots of D.heterocarpon. In addition,P deficiency treatment significantly increased the accumulation of 8 organic acids and 4 sugars and alcohols in the root system. In conclusion,D.heterocarpon improves its adaptation to P deficiency stress by promoting root growth and adjusting metabolite accumulation. This study will provide a theoretical reference for further analysis of the mechanism of adaptation to P deficiency stress in D.heterocarpon.

Key words: Desmodium heterocarpon, Phosphorus deficiency, Root morphology, Organic acids, dAMP

摘要： 为探究假地豆（Desmodium heterocarpon）对缺磷胁迫的生理和代谢响应，本研究通过水培试验，分析了正常供磷（+P处理）和缺磷（-P处理）对假地豆生长及代谢物积累的影响。结果表明：缺磷胁迫降低了假地豆地上部干重和磷含量，但促进了根系生长。随后，代谢组共检测到假地豆叶片和根系的267个代谢物，可被分为12类。其中，叶片和根系的缺磷胁迫差异累积代谢物（DAMs）均为79个。缺磷处理导致假地豆叶片和根系的原花青素、槲皮素及其衍生物的积累显著增加，而2'-脱氧腺苷5'-单磷酸（dAMP）的积累显著减少。此外，缺磷处理使根系的8个有机酸和4个糖及醇类的积累显著增加。综上，假地豆通过促进根系生长和调整代谢物积累来提高对缺磷胁迫的适应性。本研究将为进一步解析假地豆适应缺磷胁迫的机理提供理论参考。

关键词: 假地豆, 缺磷胁迫, 根系形态, 有机酸, dAMP

CLC Number:

S541

ZHU Hou-rong, ZHENG Shao-jue, LYU Jin-hui, XU Ran-ran, LUO Li-juan, DONG Rong-shu. Metabolic Derivatives Analysis of Desmodium heterocarpon in Response to Phosphorus Deficiency Stress[J]. Acta Agrestia Sinica, 2022, 30(6): 1422-1429.

朱厚荣, 郑韶爵, 吕金慧, 徐然然, 罗丽娟, 董荣书. 假地豆响应缺磷胁迫的代谢衍生物分析[J]. 草地学报, 2022, 30(6): 1422-1429.

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Metabolic Derivatives Analysis of Desmodium heterocarpon in Response to Phosphorus Deficiency Stress

假地豆响应缺磷胁迫的代谢衍生物分析

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