Abstract: In order to explore a new way of utilization of daylily stem and leaf feed resource. In this experiment, daylily by-products were mixed with silage corn according to the mass ratios of 100∶0 (T₁), 80∶20 (T₂), 60∶40 (T₃), 40∶60 (T₄), 20∶80 (T₅) and 0∶100 (T₆) for 70 days of fermentation, sensory identification, nutrient composition analysis, quality evaluation and microbial diversity analysis were carried out on different proportions of mixed forage. The results showed that compared to daylily stem and leaf single silage, the mixed silage of daylily stem and leaf and silage corn was beneficial to improve the smell and structure of silage. Based on the grey correlation analysis of 16 silage indicators, it was found that the quality of mixed silage of daylily stem and leaf and silage corn was better than that of daylily stem and leaf silage. At the bacterial genus level, a total of 1192 groups were classified, among which Lactiplantibacillus, Pantoea and unclassified Enterobacter (unclassified_Enterobacteriacea) were the dominant genera in all the mixed silage patterns. Combined to the results of RDA analysis, it was found that Ammonium nitrogen content (ANC) had a more significant effect on the microbial community composition of each sample. To sum up, from the angle of maximizing utilization of stems and leaves of daylily, T₄ proportion silage is the best.

Key words: Daylily stem and leaf, Silage corn, Mixed silage, Silage quality evaluation, Microbial diversity analysis

摘要： 为探索黄花菜(Hemerocallis citrina Baroni)茎叶饲料资源化利用的新途径，本试验将黄花菜茎叶与青贮玉米按质量比100∶0(T₁)，80∶20(T₂)，60∶40(T₃)，40∶60(T₄)，20∶80(T₅)，0∶100(T₆)进行混合青贮，发酵70 d后，对各样品进行感官鉴定、品质评价和微生物多样性分析。结果表明：与黄花菜茎叶单贮相比，黄花菜茎叶与青贮玉米混合青贮有利于改善青贮饲料的气味和结构，并且基于对16个青贮指标的灰色关联度分析发现，黄花菜茎叶与青贮玉米混合青贮品质优于黄花菜茎叶单贮。在细菌属水平上，共有1192个类群被分类，其中，植物乳杆菌属(Lactiplantibacillus)、泛菌属(Pantoea)、未分类的肠杆菌属(unclassified_Enterobacteriacea)是所有混合青贮模式中的优势属，结合冗余分析结果发现，氨态氮含量对各样品微生物群落组成影响更显著。综上考虑，从黄花菜茎叶利用最大化角度出发，以黄花菜茎叶与青贮玉米按质量比40∶60进行混合青贮为佳。

关键词: 黄花菜茎叶, 青贮玉米, 混合青贮, 青贮质量评价, 微生物多样性分析