Identification and Expression Patterns Analysis of LysM-RLKs in Response to Colletotrichum gloeosporioides and Low Phosphorus Stress in Stylosanthes guianensis

doi:10.11733/j.issn.1007-0435.2025.04.006

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (4): 1058-1071.DOI: 10.11733/j.issn.1007-0435.2025.04.006

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Identification and Expression Patterns Analysis of LysM-RLKs in Response to Colletotrichum gloeosporioides and Low Phosphorus Stress in Stylosanthes guianensis

LONG Yun-piao^1,2, ZHANG Jian-yu^1,2,3, LUO Li-juan^1,2, LIU Pan-dao³, JIANG Ling-yan^1,2

1. School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan Province 570228, China;
2. Sanya Institute Breeding and Multiplication, Hainan University, Sanya, Hainan Province 572025, China;
3. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences &Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs &Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, Hainan Province 571101, China

Received:2024-11-29 Revised:2025-02-10 Online:2025-04-15 Published:2025-04-28

柱花草响应炭疽菌和低磷胁迫的LysM-RLKs成员鉴定及表达模式分析

龙云飘^1,2, 张建禹^1,2,3, 罗丽娟^1,2, 刘攀道³, 蒋凌雁^1,2

1. 海南大学热带农林学院, 海南海口 570228;
2. 海南大学三亚南繁研究院, 海南三亚 572025;
3. 中国热带农业科学院热带作物品种资源研究所&农业农村部华南作物基因资源与种质创制重点实验室&海南省热带作物资源遗传改良与创新重点实验室, 海南海口 571101

通讯作者: 蒋凌雁,E-mail:lyjiang@hainanu.edu.cn
作者简介:龙云飘（1997-），女，汉族，海南海口人，硕士研究生，主要从事牧草基因功能研究，E-mail：longyunpiao1997@163.com
基金资助:
国家自然科学基金青年项目（32201449）；国家自然科学基金地区项目（31960342）；海南省科技人才创新项目（KJRC2023C22）；协同创新中心科研项目（XTCX2022NYC02）资助

Abstract

Abstract: To identify the members of the LysM-RLKs family of Stylosanthes guianensis （Stylo） in response to Colletotrichum gloeosporioides and low phosphorus stress， we used transcriptome data inoculated with Colletotrichum gloeosporioides and treated with low phosphorus to identify and name LysM-RLKs family members， and predict their subcellular location and protein sequence by bioinformatics methods. The specificity of tissue expression and the expression patterns in response to chitin， anthrax and low phosphorus treatment were analyzed through the related tests of Stylosanthes plants. The results showed that 11 genes， members of LysM-RLKs family， were characterized by using the corresponding transcriptome data， namely SgLYK1， SgLYK2a， SgLYK2b， SgLYK3a， SgLYK3b， SgLYK4a， SgLYK4b， SgLYK5， SgLYK7， SgLYK8 and SgNFR5. The prediction of protein localization showed that most of LysM-RLKproteins were localized on the plasma membrane， contained typical extracellular LysM domains， transmembrane domains and intracellular kinase domains. The prediction of protein structure showed that alpha helix and random coil were the major secondary structure of the proteins， and the LysM-RLKproteins were different in terms of the three-dimensional structure. The gene expression pattern analysis showed that all the identified LysM-RLKs members had tissue-specific expression pattern. The SgLYK1， SgLYK4a， SgLYK5， SgLYK7 and SgLYK8 may play important roles in responding to C. gloeosporioides infection and chitin-induced immune responses， while SgLYK3a may be involved in the responses to low phosphorus stress. This study could provide candidate genes for the breeding of anthracnose- and low phosphorus-resistant cultivars of stylo.

Key words: Stylosanthes guianensis, LysM-RLK family, Anthracnose, Low phosphorus stress, Expression patterns

摘要： 为挖掘柱花草（Stylosanthes guianensis）的LysM-RLKs家族成员，本研究利用接种胶孢炭疽菌（Colletotrichum gloeosporioides）和低磷处理的转录组数据，通过生物信息学方法鉴定和命名LysM-RLKs家族成员，预测其亚细胞定位和蛋白序列；通过柱花草植株相关试验，进行组织表达特异性及响应几丁质、炭疽菌和低磷处理的表达模式分析。结果表明，共鉴定到11个SgLysM-RLKs成员，命名为SgLYK1，SgLYK2a，SgLYK2b，SgLYK3a，SgLYK3b，SgLYK4a，SgLYK4b，SgLYK5，SgLYK7，SgLYK8和SgNFR5；其蛋白大多定位于质膜，含有典型的胞外LysM、跨膜和胞内激酶结构域，蛋白二级结构以α螺旋和无规卷曲为主，蛋白三维结构存在差异；该家族成员均存在组织特异性，其中SgLYK1，SgLYK4a，SgLYK5，SgLYK7和SgLYK8可能在炭疽菌侵染和几丁质诱导的免疫反应中发挥重要功能，SgLYK3a则可能参与响应低磷胁迫。本研究为SgLysM-RLKs家族成员的功能研究提供参考，并为柱花草抗炭疽菌和低磷胁迫育种提供候选基因。

关键词: 柱花草, LysM-RLK家族, 炭疽病, 低磷胁迫, 表达模式

CLC Number:

S435.4

LONG Yun-piao, ZHANG Jian-yu, LUO Li-juan, LIU Pan-dao, JIANG Ling-yan. Identification and Expression Patterns Analysis of LysM-RLKs in Response to Colletotrichum gloeosporioides and Low Phosphorus Stress in Stylosanthes guianensis[J]. Acta Agrestia Sinica, 2025, 33(4): 1058-1071.

龙云飘, 张建禹, 罗丽娟, 刘攀道, 蒋凌雁. 柱花草响应炭疽菌和低磷胁迫的LysM-RLKs成员鉴定及表达模式分析[J]. 草地学报, 2025, 33(4): 1058-1071.

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Identification and Expression Patterns Analysis of LysM-RLKs in Response to Colletotrichum gloeosporioides and Low Phosphorus Stress in Stylosanthes guianensis

柱花草响应炭疽菌和低磷胁迫的LysM-RLKs成员鉴定及表达模式分析

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