Effects of Main Allelochemicals from Euphorbia jolkinii Boiss on Soil Nitrogen Transformation of Subalpine Meadow

doi:10.11733/j.issn.1007-0435.2025.04.013

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (4): 1137-1144.DOI: 10.11733/j.issn.1007-0435.2025.04.013

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Effects of Main Allelochemicals from Euphorbia jolkinii Boiss on Soil Nitrogen Transformation of Subalpine Meadow

WANG Ji-cai¹, LUO Qin², CHU Xiao-hui², DUAN Qing-song², CHEN Ping-ping³, YANG Xin², CHEN Zheng-fa², SHAN Gui-lian²

1. Datang Yunnan Power Generation Co., Ltd., Kunming, Yunnan Province 650032, China;
2. Department of Grassland Science, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;
3. Power China Kunming Engineering Corporation Limited, Kunming, Yunnan Province 650051, China

Received:2024-08-08 Revised:2024-11-28 Online:2025-04-15 Published:2025-04-28

大狼毒主要化感物质对亚高山草甸土壤氮转化的影响

王继才¹, 罗钦², 初晓辉², 段青松², 陈平平³, 杨欣², 陈正发², 单贵莲²

1. 大唐云南发电有限公司, 云南昆明 650032;
2. 云南农业大学草业科学系, 云南昆明 650201;
3. 中国电建集团昆明勘测设计研究院有限公司, 云南昆明 650051

通讯作者: 陈正发,E-mail:chenzhengfa2013@126.com;单贵莲,E-mail:shanguilian8203@126.com
作者简介:王继才（1979-），男，壮族，云南丘北人，本科，主要从事电力建设项目生态环境管理研究，E-mail：1072645493@qq.com；罗钦（1998-），男，汉族，云南宣威人，硕士研究生，主要从事毒害草化感作用研究，E-mail：2680142652@qq.com
基金资助:
云南省万人计划青年拔尖人才专项（YNWR-QNBJ-2018-031）；云南省农业联合专项（202101BD070001-068、202301BD070001-134、202301BD070001-174）；云南省水利科技项目（2023BG204001）资助

Abstract

Abstract: Euphorbia jolkinii Boiss. is a degradation indicator species of subalpine meadow， studying the effects of its invasion and expansion on soil nutrient transformation is of great significance for revealing its invasion mechanism. To investigate the effects of phenolic acids and alkaloids secreted by Euphorbia jolkinii Boiss. on soil nitrogen transformation in subalpine meadow， the indoor cultivation experiment was performed to analyze the effects of adding 0 （CK）， 10 （N10）， and 50 mg·L^-1 （N50） 4-nitrophenol solutions， as well as 0 （CK）， 100 （P100）， and 800 μl·L^-1 （P800） 2-phenylethylamine solutions on soil nitrogen content and nitrogen conversion enzyme activity in this study. The results showed that compared to CK， N10， N50， P100， and P800 treatments significantly increased the soil NO₃^--N and MBN contents， while decreased the soil NH₄⁺-N content （P<0.05）. P100 and P800 treatments significantly increased the activities of nitrate reductase and nitrite reductase， but decreased the activities of urease and acid protease （P<0.05）. N10 and N50 treatments significantly increased the activities of soil urease， acid protease， nitrate reductase， and nitrite reductase （P<0.05）. In summary， compounds such as 2-phenylethylamine secreted by Euphorbia jolkinii Boiss. facilitate the conversion of soil NH₄⁺-N to NO₃^--N， although the extent of this increase is contingent upon the type and concentration of the substance.

Key words: Poisonous plant, Euphorbia jolkinii, Allelochemicals, Soil nitrogen conversion, Invasion mechanisms

摘要： 大狼毒（Euphorbia jolkinii Boiss.）为亚高山草甸的退化指示物种，研究其入侵扩张对土壤养分转化产生的影响对揭示其入侵机制意义重大。为探明大狼毒分泌释放的酚酸、生物碱对草地土壤氮转化的影响，本研究采用室内培养实验，分析了添加0（CK）、10（N10）和 50 mg·L^-1（N50）4-硝基苯酚溶液，以及0（CK）、100（P100）和800 μl·L^-1（P800）2-苯乙胺溶液对土壤氮含量及氮转化酶活性的影响。结果表明，与CK相比，N10，N50，P100和P800 处理显著增加了土壤NO₃^--N和MBN含量，降低了NH₄⁺-N含量（P<0.05）。P100和P800处理显著提高了硝酸还原酶和亚硝酸还原酶的活性，降低了脲酶和酸性蛋白酶的活性（P<0.05）。N10和N50处理显著增加了土壤脲酶、酸性蛋白酶、硝酸还原酶和亚硝酸还原酶的活性（P<0.05）。综上，大狼毒分泌释放的2-苯乙胺等化合物会促进土壤NH₄⁺-N向NO₃^--N的转化，但提高幅度与物质种类和浓度相关。

关键词: 有毒植物, 大狼毒, 化感物质, 土壤氮转化, 入侵机制

CLC Number:

S812.2

WANG Ji-cai, LUO Qin, CHU Xiao-hui, DUAN Qing-song, CHEN Ping-ping, YANG Xin, CHEN Zheng-fa, SHAN Gui-lian. Effects of Main Allelochemicals from Euphorbia jolkinii Boiss on Soil Nitrogen Transformation of Subalpine Meadow[J]. Acta Agrestia Sinica, 2025, 33(4): 1137-1144.

王继才, 罗钦, 初晓辉, 段青松, 陈平平, 杨欣, 陈正发, 单贵莲. 大狼毒主要化感物质对亚高山草甸土壤氮转化的影响[J]. 草地学报, 2025, 33(4): 1137-1144.

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Effects of Main Allelochemicals from Euphorbia jolkinii Boiss on Soil Nitrogen Transformation of Subalpine Meadow

大狼毒主要化感物质对亚高山草甸土壤氮转化的影响

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