Screening and Characterization of Plant Growth-Promoting Bacteria from Salix Cupularis Rhizosphere in Alpine Desertification Land of Qinghai-Tibet Plateau

doi:10.11733/j.issn.1007-0435.2025.11.012

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (11): 3603-3609.DOI: 10.11733/j.issn.1007-0435.2025.11.012

Screening and Characterization of Plant Growth-Promoting Bacteria from Salix Cupularis Rhizosphere in Alpine Desertification Land of Qinghai-Tibet Plateau

DUO Shu-wei¹, HUANG Yu-xuan¹, MA Cong-yu¹, LIU Lin¹, ZHOU Ji-qiong¹, LI Xu-xu², HUANG Nan¹

1. Sichuan Agricultural University, Chengdu, Sichuan Province 610000, China;
2. Sichuan Ganzi Tibetan Autonomous Prefecture, Institute of Animal Husbandry Science, Kangding, Sichuan Province 626000, China

Received:2024-10-14 Revised:2024-11-28 Published:2025-11-13

青藏高原高寒沙化地杯腺柳根际促生菌筛选及特性研究

多姝玮¹, 黄雨璇¹, 马骢毓¹, 刘琳¹, 周冀琼¹, 李旭旭², 黄楠¹

1. 四川农业大学, 四川成都 610000;
2. 甘孜藏族自治州畜牧业科学研究所, 四川康定 626000

通讯作者: 马骢毓,E-mail:mcylemon@163.com
作者简介:多姝玮（1999-），女，汉族，内蒙古乌海人，硕士研究生，主要从事草地生物多样性研究，E-mail：2622879724@qq.com；黄雨璇（2002-），女，汉族，云南大理人，本科生，主要从事草地生物多样性研究，E-mail：1402197885@qq.com
基金资助:
四川省自然基金（2022NSFSC0110）；国家自然科学基金（32171616）；四川省科技厅面上项目（2023ZYD0055）资助

Abstract

Abstract: In this study， excellent plant growth-promoting rhizobacteria （PGPR） were screened from the rhizosphere of artificially planted Salix cupularis cuttings in alpine sandy land. The characteristics of dissolved organic phosphorus， dissolved inorganic phosphorus， nitrogen fixation and hormone secretion of these strains were studied. Superior strains were identified by molecular biology， and the strains with excellent comprehensive characteristics were verified by pot experiment. The results showed that 18 strains of organophosphorus solubilizing bacteria were determined with the amount of dissolved phosphorus ranging from 88.31 to 405.63 μg·mL^-1. There were 18 strains of inorganic phosphate solubilizing strains， and the amount of phosphate solubilized was 28.61-946.36 μg·mL^-1. There were 11 strains of nitrogen-fixing bacteria with nitrogenase activity of 36.08-231.14 nmol·（h·mL）^-1， and 8 strains secreted indole-3-acetic （IAA） with secretion of 10.10-22.27 μg·mL^-1. Through molecular biology identification， the strains were identified as Pseudomonas as the main species. The pot experiment showed that three strains， M5FRS， 5FRP and P20FRP had better comprehensive growth-promoting effect. The excellent PGPR obtained in this study will be used for the subsequent preparation of regionally targeted microbial agents. This study provides theoretical and technical support for plant growth promotion， sandy land restoration and microbial fertilizer development and utilization in alpine regions.

Key words: Alpine sandy land, Plant growth-promoting rhizobacteria, Growth-promoting characteristics of strains, Microbial inoculant

摘要： 本研究从高寒沙化地人工扦插杯腺柳（Salix cupularis）根际筛选优良植物根际促生菌（Plant growth promoting rhizobacteria，PGPR），研究菌株溶解有机磷、溶解无机磷、固氮、分泌激素特性，对优良菌株进行分子生物学鉴定，并将综合特性优良的菌株进行盆栽效果验证。结果表明，筛选获得溶有机磷菌株18株，溶磷量为88.31~405.63 μg·mL^-1；溶无机磷菌株18株，溶磷量为28.61~946.36 μg·mL^-1；固氮菌株11株，固氮酶活性为36.08~231.14 nmol·（h·mL）^-1，分泌吲哚乙酸（Indole-3-acetic，IAA）菌株8株，分泌量为10.10~22.27 μg·mL^-1。经分子生物学鉴定，菌株以假单胞菌属（Pseudomonas）为主。盆栽效果验证表明，M5FRS，5FRP，P20FRP三株菌株综合促生效果较好。本研究获得的优良PGPR将用于后续具有地区针对性的微生物菌剂的制备，从而为高寒地区植物促生、沙化地恢复以及微生物菌肥开发利用提供理论技术支持。

关键词: 高寒沙化地, 植物根际促生菌, 菌株促生特性, 微生物菌剂

CLC Number:

S144

DUO Shu-wei, HUANG Yu-xuan, MA Cong-yu, LIU Lin, ZHOU Ji-qiong, LI Xu-xu, HUANG Nan. Screening and Characterization of Plant Growth-Promoting Bacteria from Salix Cupularis Rhizosphere in Alpine Desertification Land of Qinghai-Tibet Plateau[J]. Acta Agrestia Sinica, 2025, 33(11): 3603-3609.

多姝玮, 黄雨璇, 马骢毓, 刘琳, 周冀琼, 李旭旭, 黄楠. 青藏高原高寒沙化地杯腺柳根际促生菌筛选及特性研究[J]. 草地学报, 2025, 33(11): 3603-3609.

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Screening and Characterization of Plant Growth-Promoting Bacteria from Salix Cupularis Rhizosphere in Alpine Desertification Land of Qinghai-Tibet Plateau

青藏高原高寒沙化地杯腺柳根际促生菌筛选及特性研究

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