Effects of Nitrogen and Phosphorus Addition and Arbuscular Mycorrhizal Fungi on the Biomasses and C∶N∶P Ratios of Dominant Plants in Desert Steppe in Ningxia

doi:10.11733/j.issn.1007-0435.2026.02.028

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (2): 663-669.DOI: 10.11733/j.issn.1007-0435.2026.02.028

Effects of Nitrogen and Phosphorus Addition and Arbuscular Mycorrhizal Fungi on the Biomasses and C∶N∶P Ratios of Dominant Plants in Desert Steppe in Ningxia

HE Jia¹, WANG Sheng-ze¹, ZHAO Kang¹, WANG Hong¹, HOU Wen-hui¹, SHEN Yue^1,2, YANG Xin^1,2

1. College of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia 750021, China;
2. Ningxia Grassland and Animal Husbandry Engineering Technology Research Center, Yinchuan, Ningxia 750021, China

Received:2025-03-19 Revised:2025-04-14 Published:2026-01-22

氮磷添加与AMF对宁夏荒漠草原优势植物生物量和C∶N∶P的影响

何嘉¹, 王圣泽¹, 赵康¹, 王红¹, 候文慧¹, 沈月^1,2, 杨鑫^1,2

1. 宁夏大学林业与草业学院, 宁夏银川 750021;
2. 宁夏草牧业工程技术研究中心, 宁夏银川 750021

通讯作者: 杨鑫，E-mail:yangxin@nxu.edu.cn
作者简介:何嘉（2004-），女，汉族，甘肃白银人，本科生，主要从事草地管理与生态方面的研究，E-mail:hj19109439059@163.com；
基金资助:
国家自然科学基金（32460354）；宁夏自然科学基金项目（2023AAC05012，2023AAC03044）；中国科学院“西部之光”青年学者项目（XAB2022YW15）；宁夏回族自治区一流学科（草学）建设项目（NXYLXK2017A01）资助

Abstract

Abstract: Nitrogen （N） and phosphorus （P） additions are vital management methods to alleviate plant nutrient limitation and improve grassland productivity. Soil nutrient enrichment may potentially alter the symbiotic relationships between plants and arbuscular mycorrhizal fungi （AMF）. This study conducted a three-factor experiment to study the independent and interactive effects of N and P additions and AMF on the biomass and C∶N∶P of Agropyron mongolicum and Astragalus melilotoides in the desert steppe in Ningxia. We used a kind of fungicide（benomyl）to regulate native AMF activities. Our results showed that N and P additions significantly increased the aboveground biomass of A. mongolicum and A. melilotoides， but AMF did not alter them. AMF significantly reduced the aboveground biomass of A. mongolicum under P alone addition and both N and P addition treatments. AMF increased the C∶N of A. mongolicum plants and the C∶P of A. melilotoides. P addition significantly reduced the C∶P and N∶P of two plants， and alleviated the P limitation of A. mongolicum. In summary， compared with AMF， N and P addition was more beneficial to dominant plants biomass in the desert steppe in Ningxia， and could alleviate the nutrient limitation of A. mongolicum and A. melilotoides.

Key words: Agropyron mongolicum, Astragalus melilotoides, Ecological stoichiometric ratio, Nutrient content, Phosphorus limitation

摘要： 氮磷添加是缓解温性荒漠草原生产力养分限制，提高草原生产力的重要措施之一。土壤氮磷养分富集潜在改变植物与丛枝菌根真菌（Arbuscular mycorrhizal fungi，AMF）的共生关系。本研究开展三因素原位控制试验，采用AMF抑菌剂添加调控AMF活性，分析氮磷添加与AMF的独立及交互效应对宁夏荒漠草原沙芦草（Agropyron mongolicum）与草木樨状黄芪（Astragalus melilotoides）生物量与C∶N∶P的影响。结果表明：氮磷添加显著增加沙芦草与草木樨状黄芪地上生物量。对照处理下，AMF对芦草与草木樨状黄芪地上生物量无显著影响；单独加磷与氮磷添加处理下，AMF显著抑制沙芦草地上生物量。AMF提高沙芦草植株C∶N与草木樨状黄芪C∶P。磷添加显著降低沙芦草与草木樨状黄芪C∶P与N∶P，缓解了沙芦草的磷限制。综上所述，与AMF相比，氮磷添加更利于宁夏荒漠草原优势植物生物量累积，缓解沙芦草与草木樨状黄芪的养分限制。

关键词: 沙芦草, 草木樨状黄芪, 生态化学计量比, 养分含量, 磷限制

HE Jia, WANG Sheng-ze, ZHAO Kang, WANG Hong, HOU Wen-hui, SHEN Yue, YANG Xin. Effects of Nitrogen and Phosphorus Addition and Arbuscular Mycorrhizal Fungi on the Biomasses and C∶N∶P Ratios of Dominant Plants in Desert Steppe in Ningxia[J]. Acta Agrestia Sinica, 2026, 34(2): 663-669.

何嘉, 王圣泽, 赵康, 王红, 候文慧, 沈月, 杨鑫. 氮磷添加与AMF对宁夏荒漠草原优势植物生物量和C∶N∶P的影响[J]. 草地学报, 2026, 34(2): 663-669.

References

[1] DU E Z，TERRER C，PELLEGRINI A F A，et al. Global patterns of terrestrial nitrogen and phosphorus limitation[J]. Nature Geoscience，2020，13（3）：221-226
[2] LI W B，GAN X L，JIANG Y，et al. Nitrogen effects on grassland biomass production and biodiversity are stronger than those of phosphorus[J]. Environmental Pollution，2022，309：119720
[3] CARROLL O，BATZER E，BHARATH S，et al. Nutrient identity modifies the destabilising effects of eutrophication in grasslands[J]. Ecology Letters，2022，25（4）：754-765
[4] SEABLOOM E W，BORER E T，TILMAN D. Grassland ecosystem recovery after soil disturbance depends on nutrient supply rate[J]. Ecology Letters，2020，23（12）：1756-1765
[5] SHI L N，LIN Z R，WEI X T，et al. Precipitation increase counteracts warming effects on plant and soil C：N：P stoichiometry in an alpine meadow[J]. Frontiers in Plant Science，2022，13：1044173
[6] SUN Y，WANG C T，CHEN X L，et al. Phosphorus additions imbalance terrestrial ecosystem C：N：P stoichiometry[J]. Global Change Biology，2022，28（24）：7353-7365
[7] ELSER J J，STERNER R W，GOROKHOVA E，et al. Biological stoichiometry from genes to ecosystems[J]. Ecology Letters，2000，3（6）：540-550
[8] STERNER R W，ELSER J J. Ecological stoichiometry： the biology of elements from molecules to the biosphere[M]. Princeton： Princeton University Press，2002：11-19
[9] HAN W X，FANG J Y，GUO D L，et al. Leaf nitrogen and phosphorus stoichiometry across 753 terrestrial plant species in China[J]. New Phytologist，2005，168（2）：377-385
[10] ZHAN S X，WANG Y，ZHU Z C，et al. Nitrogen enrichment alters plant N：P stoichiometry and intensifies phosphorus limitation in a steppe ecosystem[J]. Environmental and Experimental Botany，2017，134：21-32
[11] CAMENZIND T，PHILIPP GRENZ K，LEHMANN J，et al. Soil fungal mycelia have unexpectedly flexible stoichiometric C：N and C：P ratios[J]. Ecology Letters，2021，24（2）：208-218
[12] HE S B，LONG M X，HE X Q，et al. Arbuscular mycorrhizal fungi and water availability affect biomass and C：N：P ecological stoichiometry in alfalfa （Medicago sativa L.） during regrowth[J]. Acta Physiologiae Plantarum，2017，39（9）：199
[13] BENNETT A E，GROTEN K. The costs and benefits of plant-arbuscular mycorrhizal fungal interactions[J]. Annual Review of Plant Biology，2022，73：649-672
[14] KIERS E T，DUHAMEL M，BEESETTY Y，et al. Reciprocal rewards stabilize cooperation in the mycorrhizal symbiosis[J]. Science，2011，333（6044）：880-882
[15] ZHANG S J，LEHMANN A，ZHENG W S，et al. Arbuscular mycorrhizal fungi increase grain yields：a meta-analysis[J]. New Phytologist，2019，222（1）：543-555
[16] GRMAN E，ROBINSON T M P. Resource availability and imbalance affect plant-mycorrhizal interactions：a field test of three hypotheses[J]. Ecology，2013，94（1）：62-71
[17] LIU Z H，LI M H，LIU J B，et al. Higher diversity and contribution of soil arbuscular mycorrhizal fungi at an optimal P-input level[J]. Agriculture，Ecosystems & Environment，2022，337：108053
[18] YANG X，LIU Y，TIAN H Y，et al. Short-term nitrogen and phosphorus additions rather than mycorrhizal suppression determine plant community composition and productivity in desert steppe[J]. Applied Soil Ecology，2021，168：104144
[19] CUI H Y，SUN W，DELGADO-BAQUERIZO M，et al. Phosphorus addition regulates the responses of soil multifunctionality to nitrogen over-fertilization in a temperate grassland[J]. Plant and Soil，2022，473（1）：73-87
[20] JOHNSON N C. Resource stoichiometry elucidates the structure and function of arbuscular mycorrhizas across scales[J]. New Phytologist，2010，185（3）：631-647
[21] YANG G W，ROY J，VERESOGLOU S D，et al. Soil biodiversity enhances the persistence of legumes under climate change[J]. New Phytologist，2021，229（5）：2945-2956
[22] GUO Y M，ZHOU M，SHENG J，et al. Aboveground net primary productivity was not limited by phosphorus in a temperate typical steppe in Inner Mongolia[J]. Journal of Plant Ecology，2023，16（4）：rtac085
[23]
[24] MEI L L，YANG X，ZHANG S Q，et al. Arbuscular mycorrhizal fungi alleviate phosphorus limitation by reducing plant N：P ratios under warming and nitrogen addition in a temperate meadow ecosystem[J]. Science of the Total Environment，2019，686：1129-1139
[25] DING Q，HE J，GUO W L，et al. Effects of nitrogen，phosphorus and fungicide additions on net primary productivity in desert steppe[J]. Chinese Journal of Grassland，2024，46（4）：25-32 丁琴，何嘉，郭文龙，等. 添加氮磷与菌根抑菌剂对荒漠草原草地净初级生产力的影响[J]. 中国草地学报，2024，46（4）：25-32
[26] KOERSELMAN W，MEULEMAN A F M. The vegetation N：P ratio：a new tool to detect the nature of nutrient limitation[J]. Journal of Applied Ecology，1996，33（6）：1441

Effects of Nitrogen and Phosphorus Addition and Arbuscular Mycorrhizal Fungi on the Biomasses and C∶N∶P Ratios of Dominant Plants in Desert Steppe in Ningxia

氮磷添加与AMF对宁夏荒漠草原优势植物生物量和C∶N∶P的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XU Ai-yun, QIN Yi-tong, ZHANG Yuan-hong, LI Tian, CAO Bing. Effects of Elevated CO₂ Concentration on the Growth of Agropyron mongolicum Under Short-Term Nitrogen Addition Scenario [J]. Acta Agrestia Sinica, 2025, 33(4): 1211-1217.
[2]	LI Chang-ran, LIU Jing-yu, LI Ruo-hong, MAO Pei-sheng. Effects of Biochar Encrusting on Drought Resistance Characteristics of Agropyron Mongolicum during Seed Germination [J]. Acta Agrestia Sinica, 2025, 33(3): 960-967.
[3]	MA Xue-jun, ZUO Yu-zhen, LIU Rui-gang, CHEN Ping, XIE Jian-peng, WANG Fei, HE Zhen-fu. Evaluation of Productive Performance and Nutritional Value of 20 Silage Maize Varieties in Southeastern Gansu [J]. Acta Agrestia Sinica, 2024, 32(8): 2620-2629.
[4]	LI Wei, WANG Jun, XU Ru-min, DU Shi-zhou, QIAO Yu-qiang, CHEN Huan. Effects of Sludge Biochar Addition on Ryegrass and Soil Nutrient Properties [J]. Acta Agrestia Sinica, 2024, 32(4): 1295-1302.
[5]	ZHAI Yong-qing, MA Yan-hong, FAN Bo-bo, FANG Yong-yu, ZHANG Xue-feng, YU Zhuo, LI Kun, WU Jing, YAN Xiu-xiu, ZHAO Yan. Bioinformatics Identification and Expression Analysis of Dehydratin Genes in Agropyron mongolicum [J]. Acta Agrestia Sinica, 2024, 32(3): 684-692.
[6]	FAN Lu, TANG Jun, MA Lin, ZHANG Zhi-peng, JIANG Qing-xue, LIE Ren-cuo, MA Qiao-ling, WANG Xue-min, ZHAO Wei. Screening Genes Related to Drought Resistance in Agropyron mongolicum Keng Based on Transcriptome [J]. Acta Agrestia Sinica, 2024, 32(11): 3344-3357.
[7]	WANG Xu-cheng, WANG Xing, WANG Qin, SONG Wen-xue, WANG Tong-Rui, HUANG Shu-di, WANG Zhi-yuan, FU Bing-zhe, GAO Xue-qin. Effects of Row Spacing and Seeding Rate on Seed Yield and Its Components of Agropyron mongolicum [J]. Acta Agrestia Sinica, 2023, 31(9): 2882-2889.
[8]	ZHANG Ya-qiang, XIE Ting-ting, LIANG Guan-jun. Effects of Precipitation Changes on Soil Stoichiometric Characteristics under Reaumuria soongorica Litter Decomposition [J]. Acta Agrestia Sinica, 2023, 31(5): 1445-1453.
[9]	LI Yong-kang, LIU Jin-long, XU Dong-mei, SA Chun-ning, LIU Meng-ge, MA Yuan. Resource Allocation Characteristics of Agropyron mongolicum in the Desert Steppe in Ningxia [J]. Acta Agrestia Sinica, 2023, 31(4): 1125-1133.
[10]	LIU Yan, CHEN Meng-jiao, GUO Tong-tong, CUI Jing, LI Lan-ping, LIANG De-fei, LI Yong-hui, ZHANG Zhen-hua, ZHU Xiao-xue, REN Fei. Effects of Multi-gradient Nitrogen and Phosphorus Additions on Biomass and Nitrogen and Phosphorus Content of Alpine Meadow Plant Community [J]. Acta Agrestia Sinica, 2023, 31(3): 751-759.
[11]	ZHANG Jia-yu, LI Xiao-wei, LIU Wan-di, HUANG Wen-guang, MA Xiao-jing, YANG Li-bin. A Study on the Agropyron mongolicum Community Classification and the Relationship Between that Community Characteristics and the Environment in Ningxia [J]. Acta Agrestia Sinica, 2023, 31(2): 498-509.
[12]	MA Ya-chun, ZHANG Jie, GAO Ya-min, YAO Tuo, LI Hai-yun, LI Chang-ning, JIN Yan-li. Comprehensive Evaluation on Soil Nutrient Change Characteristics and Quality of Different Degraded Grasslands in Qilian Mountains [J]. Acta Agrestia Sinica, 2022, 30(7): 1621-1629.
[13]	ZHAO Mu-qier, WANG Zhi-jun, BAO Jian, SUN Lin, ZHANG Yong-hu, WEN Rui, WANG Wei, JIA Yu-shan, GE Gen-tu. Effect of Different Silage Additives on the Silage Quality of Forage Millet [J]. Acta Agrestia Sinica, 2022, 30(4): 1021-1026.
[14]	DING Si-jia, CHENG Mo-xiang, XIE Shuang-quan, WANG Xiu-suang, HAO Xing-ming, ZHUANG Li. Response of Growth Characteristics and Chlorophyll Fluorescence Characteristics of Ephemeral Plants to Different Soil Types [J]. Acta Agrestia Sinica, 2022, 30(12): 3308-3316.
[15]	WU Jing, FAN Bo-bo, ZHANG Xue-feng, SONG Jin-wei, FANG Yong-yu, YU Zhuo, YAN Xiu-xiu, ZHAO Yan, MA Yan-hong. Bioinformatics and Expression Analysis of ERF Transcription Factor in Agropyron mongolicum Keng [J]. Acta Agrestia Sinica, 2022, 30(11): 2910-2921.