The Effect of Exogenous Selenium on the Germination and Physiological Characteristics of Alfalfa Seedlings under Mixed Salt Alkali Stress

doi:10.11733/j.issn.1007-0435.2026.08.019

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (8): 2960-2970.DOI: 10.11733/j.issn.1007-0435.2026.08.019

The Effect of Exogenous Selenium on the Germination and Physiological Characteristics of Alfalfa Seedlings under Mixed Salt Alkali Stress

GAO Hong-rui^1,4, YU Ya-na^1,4, FANG Zhi-hong^1,4, CHEN Xin-xing^1,4, XIA Fang-shan^1,4, SHI Yong-hong², ZHANG Yun-ting³, ZHANG Ting³, HOU Dong-lai³, YANG Zi-sen³, TONG Li-rong^1,4

1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. College of Animal Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
3. Center of Animal Husbandry Technology extension Service on Shanxi Province, Taiyuan, Shanxi 030000, China;
4. Shanxi Key Laboratory of Grassland Ecological Protection and Native Grass Germplasm Innovation, Taigu, Shanxi Province 030801, China

Received:2025-11-14 Revised:2026-03-26 Published:2026-08-04

外源硒对混合盐碱胁迫下紫花苜蓿萌发及苗期生理特性的影响

高泓瑞^1,4, 于雅娜^1,4, 方志红^1,4, 陈鑫幸^1,4, 夏方山^1,4, 石永红², 张运婷³, 张婷³, 侯东来³, 杨子森³, 佟莉蓉^1,4

1. 山西农业大学草业学院, 山西太谷 030801;
2. 山西农业大学动物科学学院, 山西太谷 030801;
3. 山西省畜牧技术推广中心, 山西太原 030000;
4. 草地生态保护与乡土草种质资源创新山西省重点实验室, 山西太谷 030801

通讯作者: 方志红,E-mail:fangzhihong2007@126.com;佟莉蓉,E-mail:tlr122500@126.com
作者简介:高泓瑞(2000-),女,汉族,山西太原人,硕士研究生,主要从事牧草抗逆性方向研究,E-mail:xiaogao001219@163.com
基金资助:
山西省基础研究计划项目（202303021222060）；国家现代农业产业技术体系（CARS-34-37）；中央引导地方科技发展资金项目（YDZJSX2022C011）；山西省重点研发计划项目（201903D221091）；山西农业大学校科技创新提升工程（CXGC2023023）资助

Abstract

Abstract: To investigate the role of exogenous selenium in regulating plant tolerance to saline-alkali stress， alfalfa （Medicago sativa L.） ‘WL298HQ’ was used as experimental material. Different concentrations of exogenous selenium were applied during seed germination and seedling stages to explore the effects on seed germination and physiological characteristics under mixed saline-alkali stress. The results showed that applying exogenous selenium at concentrations of 0.5， 1， and 2 mmol·L^-1 significantly promoted the germination of WL298HQ seeds under stress. The most pronounced promoting effect on seed germination was observed at 0.5 mmol·L^-1. Comprehensive analysis of various physiological indicators at the seedling stage revealed that 2 mmol·L^-1 exogenous selenium effectively alleviated the physiological damage caused by mixed saline-alkali stress to alfalfa seedlings. Mixed saline-alkali stress significantly inhibited the antioxidant capacity and increased cell membrane permeability of alfalfa. However， exogenous selenium application under mixed saline-alkali stress promoted plant growth， reduced the degree of membrane lipid peroxidation， and simultaneously enhanced the activities of various antioxidant enzymes in the leaves.

Key words: Alfalfa, Mixed saline-alkali stress, Exogenous selenium, Physiological characteristics

摘要： 为研究外源硒在调控植物耐盐碱方面的作用，以紫花苜蓿（Medicago sativa L.）‘WL298HQ’为试验材料，通过紫花苜蓿种子萌发和苗期施加不同浓度外源硒试验，探究外源硒对盐碱胁迫下紫花苜蓿种子萌发及苗期生理特性的影响，结果表明：施加0.5 mmol·L^-1，1 mmol·L^-1和2 mmol·L^-1的外源硒后，均对混合盐碱胁迫下的WL298HQ种子的萌发有明显的促进作用，当外源硒浓度为0.5 mmol·L^-1时，外源硒对WL298HQ种子萌发有显著的促进作用。对WL298HQ苗期各项生理指标进行综合分析可知，2 mmol·L^-1的外源硒能够有效减轻混合盐碱胁迫对紫花苜蓿幼苗造成的生理损伤。混合盐碱胁迫显著抑制紫花苜蓿的抗氧化能力和增加细胞膜透性，但在混合盐碱胁迫下施加外源硒处理能够促进植株生长，减轻膜脂过氧化程度，并同步增强叶片中各种抗氧化酶活性。

关键词: 紫花苜蓿, 混合盐碱胁迫, 外源硒, 生理特性

CLC Number:

S963.22+3.3

GAO Hong-rui, YU Ya-na, FANG Zhi-hong, CHEN Xin-xing, XIA Fang-shan, SHI Yong-hong, ZHANG Yun-ting, ZHANG Ting, HOU Dong-lai, YANG Zi-sen, TONG Li-rong. The Effect of Exogenous Selenium on the Germination and Physiological Characteristics of Alfalfa Seedlings under Mixed Salt Alkali Stress[J]. Acta Agrestia Sinica, 2026, 34(8): 2960-2970.

高泓瑞, 于雅娜, 方志红, 陈鑫幸, 夏方山, 石永红, 张运婷, 张婷, 侯东来, 杨子森, 佟莉蓉. 外源硒对混合盐碱胁迫下紫花苜蓿萌发及苗期生理特性的影响[J]. 草地学报, 2026, 34(8): 2960-2970.

References

[1] WANG C F，HAN G L，YANG Z R，et al. Plant salinity sensors： current understanding and future directions[J]. Frontiers in Plant Science，2022，13：859224
[2] LI H，LIN J，YANG Q S，et al. Comprehensive analysis of differentially expressed genes under salt stress in pear （Pyrus betulaefolia） using RNA-Seq[J]. Plant Growth Regulation，2017，82（3）：409-420
[3] ZHAO G M，SHI Q M，HAN Y，et al. The physiological and biochemical responses of a medicinal plant （Salvia miltiorrhiza L.） to stress caused by various concentrations of NaCl[J]. Plos One，2014，9（2）：e89624
[4] WANG X M，CHEN Z T，SUI N. Sensitivity and responses of chloroplasts to salt stress in plants[J]. Frontiers in Plant Science，2024，15：1374086
[5] LI X Y， XIE L N. Research progress in Na⁺ regulation mechanism of plants under salt stress[J]. Biotechnology Bulletin，2019，35（7）：148-155
[6] SU K Q，MU L，ZHOU T，et al. Intercropped alfalfa and spring wheat reduces soil alkali-salinity in the arid area of northwestern China[J]. Plant and Soil，2024，499（1）：275-292
[7] WANG X C，YANG T H，WANG C，et al. Effects of mixed saline-alkali stress on growth and physiology of alfalfa[J]. Chinese Agricultural Science Bulletin，2022，38（19）：139-145王晓春，杨天辉，王川，等. 混合盐碱胁迫对紫花苜蓿的生长和生理指标的影响[J]. 中国农学通报，2022，38（19）：139-145
[8] MA Y H. Response of Astragalus membranaceus seedlings to treatments of selenium and cadmium and mechanism of selenium relieving cadmium stress[D]. Harbin：Northeast Forestry University，2019：15-20马月花. 黄芪幼苗对硒、镉处理的响应以及硒缓解镉胁迫的机理研究[D]. 哈尔滨：东北林业大学，2019：15-20
[9] CITTRARASU V，KALIANNAN D，DHARMAN K，et al. Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity，antimicrobial，mosquitocidal and photocatalytic activities[J]. Scientific Reports，2021，11：1032
[10] WANG M，ALI F，WANG M K，et al. Understanding boosting selenium accumulation in Wheat （Triticum aestivum L.） following foliar selenium application at different stages，forms，and doses[J]. Environmental Science and Pollution Research，2020，27（1）：717-728
[11] ZHANG Y W，DONG C R，LIU X Q，et al. Research on effects of selenium on the uptake and transfer of Cd，as and Hg in rice[J]. Journal of Biology，2021，38（5）：101-104，109张祎雯，董畅茹，刘小强，等. 硒对水稻吸收转运镉、砷和汞影响机制的研究进展[J]. 生物学杂志，2021，38（5）：101-104，109
[12] ADHIKARY S，BISWAS B， CHAKRABORTY D， et al. Seed priming with selenium and zinc nanoparticles modifies germination，growth，and yield of direct-seeded rice （Oryza sativa L.）[J]. Scientific Reports，2022，12：7103
[13] WANG M，WANG Y X，GE C H，et al. Foliar selenium nanoparticles application promotes the growth of maize （Zea mays L.） seedlings by regulating carbon，nitrogen and oxidative stress metabolism[J]. Scientia Horticulturae，2023，311：111816
[14] HAO S N，LIU P F，QIN J，et al. Effects of applying different doses of selenite to soil and foliar at different growth stage on selenium content and yield of different oat varieties[J]. Plants，2022，11（14）：1810
[15] SHI H P，ZHANG X Y，XU S. Effect of selenium on growth，flowering and activities of antioxidant enzymes of Arachis hypogaea seedlings under hydroponic conditions[J]. Chinese Journal of Tropical Crops，2022，43（12）：2489-2495施和平，张晓元，许晟. 水培条件下硒对花生幼苗生长和开花及抗氧化酶活性的影响[J]. 热带作物学报，2022，43（12）：2489-2495
[16] WANG H，ZHANG Y J，ZHOU W X，et al. The effects of exogenous selenium on seed germination and seedling physiological characteristics of Atractylodes macrocephala[J]. Molecular Plant Breeding，2019，17（22）：7551-7558王华，张雅娟，周武先，等. 外源硒对白术种子萌发及幼苗生理特性的影响[J]. 分子植物育种，2019，17（22）：7551-7558
[17] GUO K Y，WU Y Z，YANG C H，et al. The effects of exogenous selenium on seed germination and seedling physiological characteristics of Polygonatum sibiricum red[J]. Molecular Plant Breeding，2020，18（22）：7568-7576郭坤元，吴育中，杨春惠，等. 外源硒对黄精种子萌发及幼苗生理特性的影响[J]. 分子植物育种，2020，18（22）：7568-7576
[18] NING N，ZHANG H M，AN S P，et al. Effects of exogenous sodium selenite on leaf active oxygen metabolism and grape storage quality in Vitis vinifera L[J]. Northern Horticulture，2022（15）：89-97宁娜，张惠敏，安姝嫔，等. 外源硒对葡萄叶片活性氧代谢及果实贮藏品质的影响[J]. 北方园艺，2022（15）：89-97
[19] CHENG K，HE M，HUANG Z，et al. Effect of interaction between selenium and sulfur on osmotic regulation and antioxidant enzymes of broccoli seedlings[J]. Acta Agriculturae Boreali-occidentalis Sinica，2018，27（1）：98-107成凯，何茂，黄志，等. 硒、硫互作对青花菜幼苗渗透调节物质和抗氧化酶的影响[J]. 西北农业学报，2018，27（1）：98-107
[20] CUI X W，GUO Z P， MAO Y，et al. Evaluation on salt tolerance of 33 alfalfa varieties during germination[J]. Acta Ecologiae Animalis Domastici，2022，43（2）：55-65
[21] LI X E. Effects of Pb stress on seed germination physiology， seedling antioxidant system and Pb accumulation of alfalfa[J]. Acta Agrestia Sinica，2026，34（5）：1691-1700李小娥. Pb胁迫对紫花苜蓿种子萌发生理及幼苗抗氧化系统和Pb富集效应的影响[J].草地学报，2026，34（5）：1691-1700
[22] ARNON D I. Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris[J]. Plant Physiology，1949，24（1）：1-15
[23] HEATH R L，PACKER L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophysics，1968，125（1）：189-198
[24] SANYAL R P，PRASHAR V，JAWALI N，et al. Molecular and biochemical analysis of duplicated cytosolic CuZn superoxide dismutases of rice and in silico analysis in plants[J]. Frontiers in Plant Science，2022，13：864330
[25] ZHAO Y，LI Y S，GAO X F. A new method for accurate determination of peroxidase activity based on fluorescence decrease of guaiacol[J]. Chinese Journal of Analytical Chemistry，2015，43（7）：1040-1046
[26] YEMELYANOV V V，LASTOCHKIN V V，PRIKAZIUK E G，et al. Activities of catalase and peroxidase in wheat and rice plants under conditions of Anoxia and post-anoxic aeration[J]. Russian Journal of Plant Physiology，2022，69（6）：117
[27] KAUR Y，DAS N. Roles of polyamines in growth and development of the solanaceous crops under normal and stressful conditions[J]. Journal of Plant Growth Regulation，2023，42（8）：4989-5010
[28] FILHO E G A，BRAGA L N，SILVA L M A，et al. Physiological changes for drought resistance in different species of Phyllanthus[J]. Scientific Reports，2018，8：15141
[29] SIVAKUMAR J，PRASHANTH J E P，RAJESH N，et al. Principal component analysis approach for comprehensive screening of salt stress-tolerant tomato germplasm at the seedling stage[J]. Journal of Biosciences，2020，45（1）：141
[30] ANANNYA D，SAIKAT D，SOUJANYA J，et al. Efficacy of selenium in enhancing crop resilience against soil salinity in agroecosystems[M]. Selenium in Sustainable Agriculture： A Soil to Spoon Prospective，2025：979-1005
[31] CUI K F，DAI H P，ZHU J N，et al. Effects of selenium stress on the physiological characteristic of Medicago sativa L[J]. Crops，2016（4）：133-136崔科飞，代惠萍，朱佳妮，等. 硒胁迫对大叶紫花苜蓿生理特性的影响[J]. 作物杂志，2016（4）：133-136
[32] WANG H Q，YANG D X，LIU H E，et al. Effects of different concentrations of exogenous selenium on wheat seedlings under moderate salt stress[J]. Journal of Henan Agricultural University，2024，58（5）：791-799王浩权，杨东霞，刘红恩，等. 不同浓度外源硒对中等盐胁迫下小麦幼苗的影响[J]. 河南农业大学学报，2024，58（5）：791-799
[33] HAN J G. Forage Seed Science[M]. Beijing： China Agricultural University Press，2000：18-23韩建国. 牧草种子学[M]. 北京：中国农业大学出版社，2000：18-23
[34] PENG Q，HE H H，FAN C B，et al. Effects of selenium on seed germination and seedling growth of alfalfa[J]. Seed，2020，39（6）：1-8彭琪，何红花，范陈斌，等. 硒对紫花苜蓿种子萌发及幼苗生长的影响[J]. 种子，2020，39（6）：1-8
[35] TU F. How much solar energy can green plants convert in a year[J]. The Plant Journal，1977，6
[36] CHEN H Y，HUANG R，ZHAO L，et al. Effects of Cp2-EPS on the morphology and photosynthesis characteristics of alfalfa seedlings under salt stress[J]. Acta Agrestia Sinica，2024，32（1）：229-238陈海雁，黄荣，赵亮，等. Cp2-EPS对盐胁迫下紫花苜蓿苗期形态及光合特性的影响[J]. 草地学报，2024，32（1）：229-238
[37] THOLEN D，ZHU X G. The mechanistic basis of internal conductance： a theoretical analysis of mesophyll cell photosynthesis and CO₂ diffusion[J]. Plant Physiology，2011，156（1）：90-105
[38] ENGINEER C B，HASHIMOTO-SUGIMOTO M，NEGI J，et al. CO₂ sensing and CO₂ regulation of stomatal conductance： advances and open questions[J]. Trends in Plant Science，2016，21（1）：16-30
[39] KESAWAT M S，SATHEESH N，KHERAWAT B S，et al. Regulation of reactive oxygen species during salt stress in plants and their crosstalk with other signaling molecules-current perspectives and future directions[J]. Plants，2023，12（4）：864
[40] HOU W J，MA D M，ZHANG L，et al. Modulation of salt tolerance in Echinochloa frumentacea by foliar spraying of epibrassinolide[J]. Acta Botanica Boreali-Occidentalia Sinica，2024，44（4）：517-528侯汶君，麻冬梅，张玲，等. 叶面喷施表油菜素内酯对湖南稷子耐盐性的调控作用[J]. 西北植物学报，2024，44（4）：517-528
[41] LIU Y X，SUI X Q，WANG X Y，et al. Mitigating effects of exogenous melatonin on alfalfa under salt stress[J]. Acta Prataculturae Sinica，2025，34（9）：206-214刘沂欣，隋晓青，王鑫尧，等. 外源褪黑素对盐胁迫下紫花苜蓿的缓解作用[J]. 草业学报，2025，34（9）：206-214

The Effect of Exogenous Selenium on the Germination and Physiological Characteristics of Alfalfa Seedlings under Mixed Salt Alkali Stress

外源硒对混合盐碱胁迫下紫花苜蓿萌发及苗期生理特性的影响

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