Genome-wide Identification and Analysis of the FAR1/FHY3 Gene Family of Zoysia japonica

doi:10.11733/j.issn.1007-0435.2026.10.005

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (10): 3566-3578.DOI: 10.11733/j.issn.1007-0435.2026.10.005

Genome-wide Identification and Analysis of the FAR1/FHY3 Gene Family of Zoysia japonica

YIN Xi-ping, DONG Kang-ting, HE Fa-hui, YU Tong, YANG Zhi-min, YU Jing-jin

College of Grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China

Received:2026-04-09 Revised:2026-06-14 Published:2026-10-10

结缕草FAR1/FHY3基因家族鉴定与分析

殷希平, 董康挺, 何法慧, 俞彤, 杨志民, 于景金

南京农业大学草业学院, 江苏南京 210095

通讯作者: 杨志民, E-mail: nauyzm@njau.edu.cn;于景金, E-mail: jingjin_yu@126.com
作者简介:殷希平（2001-），男，汉族，安徽安庆人，硕士研究生，主要从事草坪草抗逆相关研究，E-mail：1549870895@qq.com
基金资助:
中央财政林业科技推广示范项目（Su [2023] TG12）资助

Abstract

Abstract: The FAR1/FHY3 transcription factor family plays a crucial regulatory role in plant phot signaling， growth and development， and stress responses. Based on whole-genome data from Zoysia japonica S.， this study aimed to systematically identify members of the FAR1/FHY3 gene family and analyze their molecular characteristics and expression patterns. The study utilized the Zoysia japonica variety 'Nannong Blue Light' as the experimental material. Family members were screened and identified from the whole genome， and their physicochemical properties， phylogenetic relationships， gene structures， conserved motifs， and promoter cis-acting elements were analyzed. Tissue-specific expression and changes in expression under shading stress were detected using RT-qPCR. The results showed that a total of 17 ZjFAR1 genes were identified， unevenly distributed across 10 chromosomes and grouped into 6 subfamilies. Members within the same subfamily exhibited highly similar gene structures and motif compositions， with Motif 2 and Motif 4 being shared by all members； the promoter regions were rich in elements associated with light response， hormone response， and abiotic stress. Tissue expression analysis revealed that ZjFAR1 genes exhibited tissue specificity， and most members showed differential expression under shading stress. Among them， ZjFAR15 was consistently upregulated， while ZjFAR14 and ZjFAR17 were consistently downregulated， suggesting they were candidate genes for the shading response. This study provides the first systematic analysis of the molecular characteristics of the FAR1/FHY3 family in Zoysia grass， offering a theoretical basis for subsequent functional validation of these genes and stress-tolerant breeding.

Key words: Zoysia japonica, FAR1/FHY3, Gene family, Genome-wide bioinformatics analysis, Expression analysis, Shade stress

摘要： FAR1/FHY3转录因子家族在植物光信号转导、生长发育及逆境应答中具有重要调控功能。本研究基于结缕草（Zoysia japonica S.）全基因组数据，旨在系统鉴定FAR1/FHY3基因家族成员并分析其分子特征与表达模式。供试材料为日本结缕草品种“南农蓝光”，从全基因组中筛选鉴定家族成员，并对其进行理化性质、系统进化、基因结构、保守基序及启动子顺式作用元件分析，并通过RT-qPCR检测组织表达及遮荫胁迫下表达变化。结果表明，共鉴定到17个ZjFAR1基因，不均匀分布于10条染色体，分为6个亚族，同一亚族成员具有高度相似的基因结构与基序组成，Motif 2和Motif 4为全部成员共有；启动子区域富含光响应、激素应答及逆境胁迫相关元件；组织表达显示ZjFAR1基因具有组织特异性，遮荫胁迫下多数成员呈差异表达，其中ZjFAR15持续上调，ZjFAR14和ZjFAR17持续下调，可作为遮荫响应候选基因。本研究首次系统解析结缕草FAR1/FHY3家族分子特征，为后续基因功能验证及抗逆育种提供理论依据。

关键词: 结缕草, FAR1/FHY3, 基因家族, 生物信息学, 表达分析, 遮荫胁迫

CLC Number:

S332.2

YIN Xi-ping, DONG Kang-ting, HE Fa-hui, YU Tong, YANG Zhi-min, YU Jing-jin. Genome-wide Identification and Analysis of the FAR1/FHY3 Gene Family of Zoysia japonica[J]. Acta Agrestia Sinica, 2026, 34(10): 3566-3578.

殷希平, 董康挺, 何法慧, 俞彤, 杨志民, 于景金. 结缕草FAR1/FHY3基因家族鉴定与分析[J]. 草地学报, 2026, 34(10): 3566-3578.

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Genome-wide Identification and Analysis of the FAR1/FHY3 Gene Family of Zoysia japonica

结缕草FAR1/FHY3基因家族鉴定与分析

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