Identification and Expression Analysis of GRAS Gene Family in Pennisetum purpureum

doi:10.11733/j.issn.1007-0435.2026.09.004

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (9): 3172-3184.DOI: 10.11733/j.issn.1007-0435.2026.09.004

Identification and Expression Analysis of GRAS Gene Family in Pennisetum purpureum

CAO Lei¹, YANG Zhong-fu², FENG Guang-yan¹, ZHANG Xin-quan¹

1. College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China;
2. College of Animal Science, Guizhou University, Guiyang, Guizhou Province 550025, China

Received:2026-02-23 Revised:2026-04-19 Published:2026-09-11

象草GRAS基因家族鉴定及表达分析

曹磊¹, 杨忠富², 冯光燕¹, 张新全¹

1. 四川农业大学草业科技学院, 四川成都 611130;
2. 贵州大学动物科学学院, 贵州贵阳 550025

通讯作者: 张新全, E-mail: zhangxq@sicau.edu.cn
作者简介:曹磊(1999-),男,汉族,山西晋中人,硕士研究生,主要从事草种质资源创新与利用方向研究,E-mail:209361903@qq.com

Abstract

Abstract: The GRAS family is a class of specific transcriptional regulatory factors widely present in plants， which play vital roles in regulating plant growth and development， as well as responses to biotic and abiotic stress. It is of great significance to explore the characteristics and functions of GRAS gene family in elephant grass （Pennisetum purpureum） and clarify their regulatory roles under low-temperature stress. Based on the genomic data of elephant grass， in this study we identified members of the GRAS gene family， and analyzed their chromosomal localization， physicochemical properties， gene structural characteristics， phylogenetic relationships， as well as the expression patterns of elephant grass materials with different cold resistance under low-temperature stress. The results showed that a total of 18 GRAS genes were identified in the elephant grass genome， which were distributed on 10 chromosomes and classified into five subfamilies via phylogenetic analysis. Cis-acting element analysis revealed that the promoter regions of 10 PpGRAS members contained regulatory elements related to low-temperature response. Collinearity analysis indicated that elephant grass shared more homologous genes with Sorghum bicolor than that with Oryza sativa. Expression pattern analysis demonstrated that the expression levels of multiple GRAS family members， especially genes such as PpGRAS7 and PpGRAS10 belonging to the LISCL subfamily， were significantly positively correlated with cold resistance capacity of materials. This study provides valuable references for further exploring the functions of elephant grass GRAS family and their response mechanisms to Low-temperature stress.

Key words: Pennisetum purpureum, GRAS gene family, low-temperature stress

摘要： GRAS家族是植物体内普遍存在的一类特异性转录调控因子，在调控植物生长发育、应对生物与非生物逆境胁迫等过程中发挥重要作用。探究象草GRAS基因家族基因的特征及功能，解析其在低温胁迫下的调控作用具有重要意义。本研究以象草基因组数据为基础，对GRAS家族成员进行鉴定，并分析染色体位置、理化特性、基因结构特征、系统进化关系等，以及低温胁迫条件下不同抗寒性象草材料的表达模式。结果表明，在象草基因组中鉴定到18个GRAS基因，分布在10条染色体上，系统进化分析将其分为5个亚家族。顺式作用元件分析表明有10个PpGRAS成员的启动子区域含有低温应答相关调控元件。共线性分析表明象草与高粱（Sorghum bicolor）的同源基因数量显著多于水稻（Oryza sativa）。表达模式分析发现GRAS基因家族中多个成员，特别是亚家族LISCL，如基因PpGRAS7、PpGRAS10等的表达模式与材料的抗寒能力呈显著正相关。研究结果为深入探讨象草GRAS家族及其低温胁迫响应提供了有价值的信息。

关键词: 象草, GRAS基因家族, 低温胁迫

CLC Number:

S813.9

CAO Lei, YANG Zhong-fu, FENG Guang-yan, ZHANG Xin-quan. Identification and Expression Analysis of GRAS Gene Family in Pennisetum purpureum[J]. Acta Agrestia Sinica, 2026, 34(9): 3172-3184.

曹磊, 杨忠富, 冯光燕, 张新全. 象草GRAS基因家族鉴定及表达分析[J]. 草地学报, 2026, 34(9): 3172-3184.

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Identification and Expression Analysis of GRAS Gene Family in Pennisetum purpureum

象草GRAS基因家族鉴定及表达分析

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