菌物学报 ›› 2019, Vol. 38 ›› Issue (5): 698-706.doi: 10.13346/j.mycosystema.180319

• 研究论文 • 上一篇    下一篇

丛枝菌根真菌对薰衣草耐热性的影响

邢红爽,孙鹏飞,李峰,李伟,郭绍霞()   

  1. 青岛农业大学园林与林学院 山东 青岛 266109
  • 收稿日期:2018-12-18 接受日期:2019-01-30 出版日期:2019-05-23 发布日期:2019-05-22
  • 通讯作者: 郭绍霞 E-mail:gsx2309@126.com
  • 基金资助:
    山东省林业科技创新项目(LYCX06-2018-34)

Effects of arbuscular mycorrhizal fungi on heat tolerance of Lavandula angustifolia

XING Hong-Shuang,SUN Peng-Fei,LI Feng,LI Wei,GUO Shao-Xia()   

  1. College of Landscape Architecture and Forestry, Agricultural University of Qingdao, Qingdao, Shandong 266109, China
  • Received:2018-12-18 Accepted:2019-01-30 Online:2019-05-23 Published:2019-05-22
  • Contact: Shao-Xia GUO E-mail:gsx2309@126.com
  • Supported by:
    Supported by Forestry Science and Technology Innovation Project of Shandong Province(LYCX06-2018-34)

摘要:

于不同温度(25℃/20℃、35℃/30℃和40℃/35℃)下测定接种丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)摩西斗管囊霉Funneliformis mosseae、变形球囊霉Glomus versiformeF. mosseae+G. versiforme 混合菌种处理对狭叶薰衣草Lavandula angustifolia耐热性的影响。结果表明,供试AMF能与狭叶薰衣草根系形成菌根共生体,以混合菌种处理的侵染率最高,达到68%。40℃/35℃下,与不接种AMF对照相比,混合菌种处理的狭叶薰衣草叶片可溶性糖、可溶性蛋白、脯氨酸和叶绿素等含量以及根系活力分别提高了46%、68%、65%、29%和70%;与不接种AMF对照相比,3种温度处理下接种AMF显著增加了狭叶薰衣草植株超氧化物歧化酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶和硝酸还原酶活性,而降低相对电导率及丙二醛含量。表明接种AMF能增强狭叶薰衣草抗氧化酶活性,减轻高温造成的伤害,增强耐热性,与单一接种相比以混合接种摩西斗管囊霉和变形球囊霉提高狭叶薰衣草耐热性的效应最大。

关键词: 狭叶薰衣草, 丛枝菌根真菌, 高温胁迫, 抗氧化酶, 渗透调节物质

Abstract:

The influence of arbuscular mycorrhizal fungi (AMF) Funneliformis mosseae, Glomus versiforme , and F. mosseae+G. versiforme on heat tolerance of Lavandula angustifolia plants was determined under the conditions of different temperatures (25°C/20°C, 35°C/30°C and 40°C/35°C). Results showed that the tested AMF could colonize L. angustifolia roots, and the mixed inoculation treatment gave the highest colonization percentage (68%). As compared with the non-inoculation control, the content of soluble sugar, soluble protein, proline and the chlorophyll in leaves and the root activity of plants treated with F. mosseae+G. versiforme under 40°C/35°C were increased by 46%, 68%, 65%, 29%, and 70%, respectively. Under the conditions of three temperature treatments, the activity of superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, and nitrate reductase in leaves of mycorrhizal plants were higher than that in control, while the content of malondialdehyde and relative conductivity were significantly lower than that of the control. The results showed that AMF could enhance the activity of antioxidant enzymes in L. angustifolia plants, alleviate the damage caused by high temperature and enhance the heat tolerance. It was considered that the mixed inoculation with F. mosseae+G. versiforme gave the most significant effect of increasing heat tolerance towards L. angustifolia plants.

Key words: Lavandula angustifolia, arbuscular mycorrhizal fungi, high temperature stress, antioxidant enzymes, osmoregulation substance