菌物学报 ›› 2021, Vol. 40 ›› Issue (5): 1110-1122.doi: 10.13346/j.mycosystema.200308

• 研究论文 • 上一篇    下一篇

丛枝菌根真菌对青杨抗溃疡病生物量和抗病酶活性的影响

张钰1,唐明2,*()   

  1. 1.西北农林科技大学林学院 陕西 杨陵 712100
    2.亚热带农业生物资源保护与利用国家重点实验室 华南农业大学林学与风景园林学院 广东 广州 510642
  • 收稿日期:2020-09-25 接受日期:2020-11-05 出版日期:2021-05-22 发布日期:2021-06-03
  • 通讯作者: 唐明 E-mail:tangm@nwsuaf.edu.cn
  • 基金资助:
    十三五国家重点研发计划(2018YFD0600203-3);国家自然科学基金(32071639)

Effects of arbuscular mycorrhizal fungi on biomass and disease-resistance enzyme activities of Cathay poplar against canker

ZHANG Yu1,TANG Ming2,*()   

  1. 1. College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
    2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, Guangdong 510642, China
  • Received:2020-09-25 Accepted:2020-11-05 Online:2021-05-22 Published:2021-06-03
  • Contact: Ming TANG E-mail:tangm@nwsuaf.edu.cn
  • Supported by:
    National Key Research and Development Program for the 13th Five-Year Plan(2018YFD0600203-3);National Natural Science Foundation of China(32071639)

摘要:

本研究以青杨Populus cathayana 1年生扦插苗为试验材料,接种丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)异形根孢囊霉Rhizophagus irregularis和杨树溃疡病菌聚生小穴壳菌Dothiorella gregaria,测定AMF对青杨根茎叶生物量,丙二醛(MDA)、脯氨酸和茎部纤维素含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)、几丁质酶、多聚半乳糖醛酸酶和果胶甲基酯酶等抗病酶活性的影响。结果表明,接种AMF可以增加青杨根茎生物量,增加茎纤维素含量来增强树势,降低溃疡病发病率;接种AMF能够显著降低感病杨树茎部和根系MDA含量,增加叶片和根系脯氨酸含量,提高根茎叶PPO活性、茎SOD活性和叶片CAT活性;接种AMF前期可以提高茎和根系几丁质酶和β-1,3-葡聚糖酶活性,也可以提高叶片和茎多聚半乳糖醛酸酶、果胶甲基酯酶活性,接种病原菌条件下AMF效果更加显著。结论认为,AMF通过积累脯氨酸,降低MDA含量,提高SOD、CAT和PPO活性,增强杨树抗溃疡病能力;同时也可提高多聚半乳糖醛酸酶和果胶甲基酯酶活性来抑制病原菌菌丝的生长,减弱菌丝的扩展能力,增强杨树抗病性。

关键词: 丛枝菌根真菌, 青杨, 溃疡病, 抗病性

Abstract:

Investigation was made on the changes of biomass, content of malonaldehyde (MDA), proline content of root, stem and leaf, cellulose content of stem, and disease-resistance enzyme activities such as activities of superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO), chitinase, polygalacturonase and pectin methyl esterase of 1-year-old cuttings of Populus cathayana after inoculation with arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis and Dothiorella gregaria, causal agent of poplar canker disease. The results indicated that AMF inoculation could promote the root and stem biomass of P. cathayana, increase cellulose content of stem to enhance tree vigor, and reduce the infection rate of canker disease. AMF inoculation could significantly decrease the content of MDA in the stem and root of infected poplar, increase the proline accumulation in leaf and root, and increase the activities of PPO in root, stem and leaf, SOD in stem and CAT in leaf. AMF inoculation not only increased chitinase and β-1,3-glucanase activities in stem and root at the early stage, but increased the activities of polygalacturonase and pectin methyl esterase in leaf and stem. The effect of AMF seemed more significant under pathogen stress. Our conclusion was that AMF could improve the disease resistance of poplar by accumulating proline, reducing MDA content, increasing SOD, CAT, and PPO activities, and promoting polygalacturonase and pectin methyl esterase activities to inhibit the growth of pathogen hyphae.

Key words: arbuscular mycorrhizal fungi, Populus cathayana, canker, disease resistance