| 引物名称 Primer | 引物序列 Oligonucleotide sequence (5′→3′) | 目的 Use |
|---|---|---|
| pyrG/F | GCCAGTACGAGTGTTGTGGAG | 扩增pyrG基因片段 Amplification of pyrG gene fragments |
| pyrG/R | GTCAGACACAGAATAACTCTC | |
| 054800 5F/F | GGTATGGACTGGCATGGATAC | 融合 PCR 正向引物 Fusion PCR forward primers |
| 054800 3F/R | GTAATATCAAGACTGCGACGCG | 融合 PCR 反向引物 Fusion PCR reverse primers |
| 054800 5F/EF | CATTGAGGAACGATGCCATTAC | 扩增AflAZF1上游同源臂片段 Amplification of AflAZF1 upstream homology arm fragment |
| 054800 5F/R | GGGTGAAGAGCATTGTTTGAGGCGTCTTTGCTATCTACTCGTCTC | |
| 054800 3F/ER | CTTCAATTGATCGTGTTGACAC | 扩增AflAZF1下游同源臂片段 Amplification of AflAZF1 downstream homology arm fragments |
| 054800 3F/F | GCATCAGTGCCTCCTCTCAGACGCCGTACTGGTCTTCTGTTCG | |
| pyrG/TR | GTCTGAGAGGAGGCACTGATGC | ΔAflAZF1验证引物 ΔAflAZF1 validation primers |
| pyrG/TF | GCCTCAAACAATGCTCTTCACCC | |
| 054800 RT/F | GCCATTCACCTGTCTGTTGGAC | AflAZF1-ORF验证引物 AflAZF1-ORF validation primers |
| 054800 RT/R | CCTACGATCCTTTCCACGAC |
