The polysaccharides of Paecilomyces tenuipes mycelia were purified by using DEAE cellulose DE-52 and Sephedex G-100, and pure polysaccharides PtPs1 and PtPs2 were obtained. The structural unit (monosaccharide) of PtPs1 and PtPs2 was analyzed by HPAEC-PAD. The results indicated that both PtPs1 and PtPs2 consisted of glucose, galactose and mannose. The infrared spectral analysis showed that PtPs1 and PtPs2 were all pyranose of α-configuration.