Mycosystema ›› 2012, Vol. 31 ›› Issue (5): 690-700.

• Papers • Previous Articles     Next Articles

Effects of long-term fertilization on arbuscular mycorrhizal fungi community in calcareous purple paddy soil

  

  • Online:2012-09-15 Published:2012-11-15
  • About author:College of Resource and Environment, Sichuan Agricultural University, Chengdu, Sichuan 611130, China;College of Resource and Environment, Sichuan Agricultural University, Chengdu, Sichuan 611130, China;College of Resource and Environment, Sichuan Agricultural University, Chengdu, Sichuan 611130, China;Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, China;Department of Applied Chemistry and Microbial, University of Helsinki, Helsinki SF-00014, Finland

Abstract: To determine the influence of long term fertilization on arbuscular mycorrhizal fungi (AMF) species diversity in calcareous purplish paddy soil, rhizosphere soil samples collected from different designed plots continuously supplied with manure only (M), nitrogen fertilizer plus manure (NM), nitrogen and phosphoric fertilizer plus manure (NPM), nitrogen, phosphoric and potassium fertilizer plus manure (NPKM), mineral nitrogen (N), nitrogen-phosphoric fertilizer (NP), nitrogen and phosphoric and potassium fertilizer (NPK) and without treatment (CK), under rice-wheat rotations for 26 years were mycologically investigated. The results showed that there are 17 species of AMF, among them, 12 species belong to Glomus, accounting for 70.5 percent of the total isolated species. Three species belong to Acaulospora, accounting for 17.6 percent of the isolated species. One species belongs to Archaeospora and one species Entrophospora, accounting for 5.9 percent of isolated species. Regardless of wheat and rice, the AMF highest infection rate emerged in the control treatment (CK). Fertilizer treatments especially using phosphorus would decrease the infection rate, spore density, relative abundance and species richness of AMF. Denaturing gradient gel electrophoresis (DGGE) profiles showed that both the AMF community structure and Shannon diversity index were the highest in the soil under CK as compared with those in the soil under any other fertilizer treatments. AMF diversity index in planting plots of wheat and rice were 2.68 and 3.02, respectively. In compared with the inorganic fertilizer treatments, the combining use of manure and inorganic fertilizer increased the AMF diversity index. The results suggested that the mixing of manure and inorganic fertilizer would be more beneficial to the development of the soil AMF.