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MYCOSYSTEMA

Mycosystema ›› 2012, Vol. 31 ›› Issue (5): 800-806.

• short communications • Previous Articles    

EST-SSR markers in Agrocybe cylindracea and its application

  

  • Online:2012-09-15 Published:2012-11-15
  • About author:School of Life Sciences and Food Engineering, Nanchang University, Nanchang, Jiangxi 330031, China;School of Life Sciences and Food Engineering, Nanchang University, Nanchang, Jiangxi 330031, China;School of Life Sciences and Food Engineering, Nanchang University, Nanchang, Jiangxi 330031, China;School of Life Sciences and Food Engineering, Nanchang University, Nanchang, Jiangxi 330031, China

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Abstract: For the development of EST-SSR markers in Agrocybe cylindracea and the application of the marker in characterization of different mushrooms, 40 pairs of primers were designed based on the expressed sequence tags obtained from the shiitake Lentinula edodes. The results showed that 13 of 28 selected primer pairs could reveal high and stable polymorphisms among Agrocybe cylindracea varieties, which accounted for 46.4% of primers selected. Amplified PCR product has a total of 81 polymorphic bands, and the number of bands’ range is 2–14 with an average of 5.86 bands. Amplified fragment length is between 100–400bp. The polymorphism information content (PIC) varied from 0.3750 to 0.8032, with an average of 0.7084. The results of this study indicated that EST-SSR markers could be used in the genetic diversity and comparative genomics studies among different edible fungus species.