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Cold stress induction of ergosterol biosynthesis in Pleurotus pulmonarius

XIE Fan, SONG Yan-Jiao, CHENG Bing, MENG Guo-Liang, HAO Jin-Bin, YE Li-Yun, WU Xiao-Ping

Mycosystema, 2019, 38(12): 2221-2231. DOI: 10.13346/j.mycosystema.190270

Fig. 8 Relative expression analysis of the genes involved in ergosteroid biosynthesis pathway in Pleurotus pulmonarius X1 under the condition of low-temperature treatment.

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Table 1 qPCR primers of Pleurotus pulmonarius X57
Fig. 1 Enriched steroid biosynthesis pathway after cold challenge for 6h. The red box is the up-regulated gene.
Fig. 2 Enriched steroid biosynthesis pathway after cold challenge for 12h. The red box is the up-regulated gene while the green is down-regulated gene.
Table 2 The genes information of the ergosteroid biosynthesis pathway in Pleurotus pulmonarius
Fig. 3 The putative ergosteroid biosynthesis pathway in Pleurotus pulmonarius.
Fig. 4 Transcriptome analysis of the genes involved in ergosteroid biosynthesis pathway genes in Pleurotus pulmonarius X57 under the condition of low-temperature treatment.
Fig. 5 Relative expression analysis of the genes involved in ergosteroid biosynthesis pathway genes in Pleurotus pulmonarius X57 under the condition of low-temperature treatment by qRT-PCR.
Fig. 6 The content of ergosteroid of Pleurotus pulmonarius strain X57 grown in cultivation bag. Letter indicates significant difference at 0.05 level.
Fig. 7 Relative expression analysis of the genes involved in ergosteroid biosynthesis pathway genes in Pleurotus pulmonarius X57 under the condition of low-temperature treatment.

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