Mycosystema

Cold stress induction of ergosterol biosynthesis in Pleurotus pulmonarius

XIE Fan, SONG Yan-Jiao, CHENG Bing, MENG Guo-Liang, HAO Jin-Bin, YE Li-Yun, WU Xiao-Ping

Mycosystema, 2019, 38(12): 2221-2231. DOI: 10.13346/j.mycosystema.190270

蛋白名称 Protein name	所属MSTRG MSTRG	基因的位置 Location	长度（正+反-） Size (F+R-)	功能 Function
PpERG1	MSTRG.1322	NODE_144:3630-5859	2230+	Squalene monooxygenase
PpERG2A	MSTRG.7051	NODE_50:60237-60915	679+	C-8 sterol isomerase
PpERG2B	MSTRG.6024	NODE_3:158541-159386	846+
PpERG2C	MSTRG.4404	NODE_26:137657-138521	865+
PpERG3	MSTRG.3899	NODE_23:172227-173465	1239-	C-5 sterol desaturase
PpERG4	MSTRG.7193	NODE_51:132649-134559	1911-	Delta (24(24(1)))-sterol reductase
PpERG5	MSTRG.9240	NODE_84:38042-39892	1851+	C-22 sterol desaturase
PpERG6	MSTRG.721	NODE_121:31574-32842	1269+	Sterol 24-C-methyltransferase
PpERG7	MSTRG.1743	NODE_159:59760-62542	2783-	Lanosterol synthase
PpERG9	MSTRG.2215	NODE_1642+NODE_175:1-854	1647+	Farnesyltransferase
PpERG11	MSTRG.1883	NODE_160:44963-47015	2053-	Sterol 14-dmethylase
PpERG24A	MSTRG.1462	NODE_144:62297-64343	2047-	Delta (14)-sterol reductase
PpERG24B	MSTRG.2417	NODE_181:37882-39501	1620+	Delta (14)-sterol reductase
PpERG25A	MSTRG.3628	NODE_236:36259-37326	1068-	Methylsterol monooxygenase
PpERG25B	MSTRG.8059	NODE_63:114581-116084	1504+	Methylsterol monooxygenase
PpERG26	MSTRG.6066	NODE_3:84909-86417	1509+	3-dehydrogenase
PpERG27	MSTRG.7026	NODE_516:5626-7092	1467-	3-keto-steroid reductase

Table 2 The genes information of the ergosteroid biosynthesis pathway in Pleurotus pulmonarius

Other figure/table from this article

Table 1 qPCR primers of Pleurotus pulmonarius X57
Fig. 1 Enriched steroid biosynthesis pathway after cold challenge for 6h. The red box is the up-regulated gene.
Fig. 2 Enriched steroid biosynthesis pathway after cold challenge for 12h. The red box is the up-regulated gene while the green is down-regulated gene.
Fig. 3 The putative ergosteroid biosynthesis pathway in Pleurotus pulmonarius.
Fig. 4 Transcriptome analysis of the genes involved in ergosteroid biosynthesis pathway genes in Pleurotus pulmonarius X57 under the condition of low-temperature treatment.
Fig. 5 Relative expression analysis of the genes involved in ergosteroid biosynthesis pathway genes in Pleurotus pulmonarius X57 under the condition of low-temperature treatment by qRT-PCR.
Fig. 6 The content of ergosteroid of Pleurotus pulmonarius strain X57 grown in cultivation bag. Letter indicates significant difference at 0.05 level.
Fig. 7 Relative expression analysis of the genes involved in ergosteroid biosynthesis pathway genes in Pleurotus pulmonarius X57 under the condition of low-temperature treatment.
Fig. 8 Relative expression analysis of the genes involved in ergosteroid biosynthesis pathway in Pleurotus pulmonarius X1 under the condition of low-temperature treatment.