Two new endophytic fungal species of Diaporthe in the leaves of Camellia oleifera
HU Yujuan#, HU Jiangxin#, ZHANG Chunguang, CHANG Xiaoyun, CHEN Mingjun*
Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036, Anhui, China
Abstract: Seventy isolates of Diporthe fungi from healthy Camellia oleifera leaves were obtained by using tissue isolation method. Based on morphological characteristics and phylogenetic analysis of five fragment sequences (ITS, TEF, TUB, CAL, HIS), eight representative isolates were identified as six species, D. biguttulata, D. eres, D. hongkongensis, D. fusicola, D. qimenensis sp. nov. and D. reniformis sp. nov. β-Type conidia were not observed in the two new species. D. reniformis produces distinct reniform conidia.
Keywords: endophytic fungi; Diaporthe; new species; morphological character; phylogenetic analysis
Diaporthe qimenensis M.J. Chen & J.X. Hu, sp. nov. Fig. 2
Fungal Name: FN571850
Etymology: the specific epithet “qimenensis” refers to Qimen County, Anhui, China, the region where this species was first discovered.
Holotype: RCEF20015
Description: Colonies on PDA growing fast, reaching 75–79 mm diameter after 7 d at 25 ℃, aerial mycelium prosperous fluffy, surface initially white, then becoming pale yellowish with black conidiomata after 14 d; color in reverse center initially white to yellow, later becoming pale brown with black dots (conidiomata) (Fig. 2A); conidiomata pycnidial, cylindrical, scattered or aggregated, sporadically immersed in medium; conidial droplets exuded from the ostioles, cream white to dark brown; conidiophores 11.4–15.4× 2.6–3.0 μm, hyaline, branched, slightly curved, attenuate towards the apex, septate; alpha conidia hyaline, aseptate, fusiform to oval, with acute tip and blunt base (Table 2), 5.0–5.8×2.5–2.9 μm (av.=5.4×2.7 μm, n=30), with 0–1 oil droplets; beta conidia not observed. Immature fruiting bodies formed on alfalfa stems.
Material examined: The holotype RCEF20015 was isolated from the healthy leaf of Camellia oleifera in Xianyu Mountains Nature Reserve, Qimen County, Anhui Province, China, located at 29°96?N and 117°27?E, at an altitude of 600 m. June 12, 2021, by J.X. Hu and Y.R. Han. It was deposited in the Research Center for Entomogenous Fungi (RCEF) at Anhui Agricultural University (ex-holotype RCEF20014).
Diaporthe reniformis M.J. Chen & J.X. Hu, sp. nov. Fig. 3
Fungal Name: FN571851
Etymology: the specific epithet “reniformis” refers to the reniform conidia.
Holotype: RCEF20016
Description: Colony on PDA growing fast, reaching 79–81 mm in diameter after 7 d at 25 ℃. Aerial mycelium prosperous fluffy, white, reverse pale yellow, producing black conidiomata; conidiomata pycnidial, cylindrical, scattered sporadically, immersed in medium. Mycelia hyaline, branched, septate, smooth-walled; conidial droplets exuded from the ostioles, brown to black; conidiophores smooth-walled. Phialides solitary or in a group of three to four borne directly on metulae, attenuate towards the apex, 10.2–14.0×2.4–3.0 μm. Alpha conidia hyaline, aseptate, reniform, 5.1–5.7×2.3–2.7 μm (av.=5.4× 2.5 μm, n=30) (Table 2), with 0–1 oil droplets; beta conidia not observed; immature fruiting bodies produced on alfalfa stems.Material examined: The holotype RCEF20016 was isolated from the healthy leaf of Camellia oleifera in Xianyu Mountains Nature Reserve, Qimen County, Anhui Province, China, located at 29°96?N and 117°27?E, at an altitude of 600 m. June 12, 2021, by J.X. Hu and Y.R. Han. It was deposited in the Research Center for Entomogenous Fungi (RCEF) at Anhui Agricultural University.
