Mycosystema. 2007, 26(2): 266-272.
Pleurotus sp.2 and Trametes gallica are high-yield strains producing lignocellulolytic enzymes and the enzyme production can peak at an early stage of fermentation of the strains. Comparative studies on lignocellulolytic enzyme production in liquid and solid culture of the strains were conducted. The results indicated that the enzyme activities of manganese-dependent peroxidases(MnPs), lignin peroxidases(LiPs), laccases(Lacs), and hemicellulases(Hcels) were the highest in Pleurotus sp.2 growing on low-nitrogen, high-carbon and high inorganic salt medium. MnPs produced by Pleurotus sp.2 reached the peak in six days. When Pleurotus sp.2 was incubated in wheat straw powder containing the liquid medium of low-nitrogen, no-carbon and high inorganic salt, the activities of manganese-dependent peroxidases and laccases reached the peaks on the tenth day, but the activities of hemicellulases reached the peak on the 40th day. The enzyme activities of laccases and lignin peroxidases were the highest in Trametes gallica growing on high-nitrogen, low-carbon and high inorganic salt medium, and the enzyme activities of manganese-dependent peroxidases, and hemicellulases were the highest in the fungus growing on low-nitrogen, high-carbon and high inorganic salt medium. When T. gallica was incubated in wheat straw powder containing liquid medium of high-nitrogen, no-carbon and high inorganic salt and containing liquid medium of low-nitrogen, no-carbon and high inorganic salt, the activities of manganese-dependent peroxidases reached the peaks on the tenth day, the activities of laccases and hemicelluloses reached the peaks on the 40th day, and the activities of lignin peroxidases reached the peaks on the 50th day. Facts have proved that Pleurotus sp.2 and Trametes gallica may be excellent candidate strains in biopulping of paper industry. It was shown that the lignocellulolytic enzyme activities produced by and the biomass-degrading ability of Pleurotus sp.2 and T. gallica were high and the highest enzyme activity appeared at the early stage of solid-state fermentation. However, the enzyme-producing mechanisms of the two incubating modes were different.
