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15 July 2008, Volume 27 Issue 4
    

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  • Mycosystema. 2008, 27(4): 483.
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    Six species of Stachybotrys from soil in the warm temperate zone of eastern China are reported, 4 of them are new to China, they are Stachybotrys breviuscula, S. cylindrospora, S. mangiferae and S. microspora. Descriptions and illustrations are given. Specimens (dried cultures) and living cultures studied are deposited in Hebarium of Shandong Agricultural University: Plant Pathology (HSAUP).
  • Mycosystema. 2008, 27(4): 489-496.
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    In this sequel of report on soil dematiaceous hyphomycetes from Mount Taibai and its surrounding area, 18 species in 10 additional genera are included. The fungi were identified from 96 isolates. Among them Scolecobasidium pallescens is a new species, while Myrothecium inundatum, Oidiodendron flavum, Oidiodendron truncatum, Scopulariopsis chartarum are new records to China. Scolecobasidium pallescens is characterized by its relatively light coloured (pale brown) conidia and conidiophores, differing from its similar species, S. microspora. Latin diagnosis is given for the new species. Brief descriptions and illustrations of the new records are provided based on Chinese isolates. The other 13 species being previously known from China in several genera are also listed. All specimens (dried cultures and slides) and living cultures studied have been deposited in the Herbarium of Shandong Agricultural University: Plant Pathology (HSAUP).
  • Mycosystema. 2008, 27(4): 497-503.
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    A total of 59 isolates of soil dematiaceous hyphomycetes belonging to 28 species in 16 genera were obtained from Hulunbuir and Xilin Gol Steppe. Scolecobasidium tuberculosum is a new species morphologically resembling S.constrictum but having conspicuously tuberculate and dark brown conidia. Latin diagnosis is given for the new species. Cordana pauciseptata, Doratomyces columnaris, Bipolaris indica, Periconia jabalpurensis and Trichocladium opacum are new to Chinese fungal flora, and brief descriptions and illustrations of them are also provided based on Chinese isolates. The other 22 species previously known from China are also listed. All studied specimens (dried cultures) and living cultures are deposited in the Herbarium of Plant Pathology, Shandong Agricultural University (HSAUP).
  • Mycosystema. 2008, 27(4): 504-509.
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    Two polypores, Ceriporia camaresiana and Tyromyces transformatus, are newly recorded from Wuyi Mountains in Fujian Province, eastern China. Ceriporia camaresiana is different from other species in Ceriporia by its white pore surface when fresh, larger pores and wide allantoid basidiospores. Tyromyces transformatus was originally described from Japan, it may be confused with Tyromyces fissilis by having similar basidiospores (4-6 × 3-4μm), however, the latter species has white to cream pore surface and larger pores (2-3 per mm). The illustrated descriptions of the two species are given based on the Chinese materials.
  • Mycosystema. 2008, 27(4): 510.
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    The present paper summarizes the knowledge of Trichaptum in China, and 10 species in the genus were found. Trichaptum brastagii was newly found in China, and its illustrated description is given based on the Chinese materials. A key to the Chinese species of the genus is given.
  • Mycosystema. 2008, 27(4): 515.
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    The knowledge of Irpex in China is summarized, and three species in the genus were found. Irpex hydnoides is newly found in China, and it is different from other species in the genus by its yellowish pore surface when fresh, and by ellipsoid basidiospores. The illustrated description of the species is given based on the Chinese materials.
  • Mycosystema. 2008, 27(4): 520-524.
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    The genus Buergenerula in driftwoods and culms of Spartina alterniflora in intertidal zone is newly recorded from China seas. Taxonomy and morphology of Buergenerula spartinae and the comparison between the species and similar species were discussed in this paper. The specimens were deposited in Mycology Herbarium at Qingdao Agricultural University (MHQAU).
  • Mycosystema. 2008, 27(4): 525-531.
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    This paper deals with a short sketch of the genus Diorygma. Six species of the genus from China are reported, and four of them, D. hieroglyphicum, D. macgregorii, D. pachygraphum and D. pruinosum are new to China. A description for each of the four species is given, and a working key to the Chinese species is also provided.
  • Mycosystema. 2008, 27(4): 532-537.
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    Three new Chinese records of dictyostelids were isolated from forest soils in Liaoning and Jilin Province. They are Polysphondylium tikaliense, P. candidum and Dictyostelium aureo-stipes. Descriptions of the three species are given based on Chinese materials.
  • Mycosystema. 2008, 27(4): 538.
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    Three new Chinese records, Acaulospora paulinae, Glomus aureum, and Pacispora robigina were found in a survey of arbuscular mycorrhizal fungi associated with Prunus mume in China. They were redescribed and illustrated in accordance with Chinese materials. These specimens were stored in the Herbarium Mycologicum Academiae Sinicae (HMAS) in Beijing.
  • Mycosystema. 2008, 27(4): 543.
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    Forty different medicinal plants were investigated for arbuscular mycorrhizal association in the Rajshahi University Campus in Bangladesh. The results indicated that 35 different plants were infected by AM (arbuscular mycorrhizal) fungi as found by trypan blue staining procedure. The percentage of root colonization by AM fungi varied from 13.3% to 100%. Mangifera indica and Morus indica have maximum percentage of colonization (100%). The intensity of root colonization were abundant in the plants belonging to the families Anacardiaceae, Asclepiadaceae, Moraceae, Leguminosae and Apocynaceae whereas the intensity of colonization of crop roots were moderate and poor belonging to Gramineae and Leguminosae. The presence of greater number of spore in soil was always associated with the incidence of abundant mycelia. In plant roots the formation of spore and mycelia was restricted by low pH. Number of mycorrhizal fungus spores ranged between 35 to100 per 100g air dried soil in different family respective soils. The frequency of mycorrhizal fungus infection showed positive correlation with soil pH, moisture, water holding capacity, texture, total nitrogen, organic carbon, phosphorus, calcium, potassium, and magnesium. Especially phosphorus and nitrogen in the soil greatly influenced the plant root infection by AM fungi.
  • Mycosystema. 2008, 27(4): 554-558.
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    The ultrastructure of conidia produced by the mycelia of Polyporus umbellatus was studied by use of scanning and transmission electron microscopy. The results suggested that only clamped (dikaryotic) mycelia produced aerial conidiophores with ovoid to rod-shaped conidia. There was a distinctive bulge near the top of the conidium. The contents of the conidia were dense. This type of conidium could be regarded as arthroconidia.
  • Mycosystema. 2008, 27(4): 559-566.
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    The effects of β-ecdysone on mycelial dry weight (MDW) and the yield of extracellular polysaccharide (EPS), intracellular polysaccharide (IPS) and cordycepin of Codyceps militaris in submerged cultivation was investigated. Adding 12mg/L of β-ecdysone to the culture medium, the MDW, the secretion volume of EPS per unit of MDW and total yield of EPS were increased by 18.5%, 181.7% and 63.2%, respectively. The yield of IPS was enhanced by 29.2%, when 1.2mg/L of β-ecdysone was added. When 4mg/L of β-ecdysone was provided, the amount of cordycepin per unit of MDW and the total yield of cordycepin were increased by 65.4% and 84.7%, respectively. These results demonstrated that β-ecdysone promoted the yield of MDW, EPS, IPS and cordycepin of Codyceps militaris mycelia, though this effect varied among different components. Significantly, β-ecdysone showed a more positive role in secondary metabolite (cordycepin) accumulation than in primary metabolite (IPS) production and growth of mycelia. Furthermore, β-ecdysone stimulated the secretion of EPS.
  • Mycosystema. 2008, 27(4): 567.
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    The aim of this study is to investigate whether Pythium guiyangense, a mosquito-killing fungus isolated in Guiyang, Guizhou Province of China in 1994, is pathogenic to plants. Six common crops, Cucumis sativa, Lycopersicon esculentum, Capsicum annuum, Nicotiana tabacum, Brassica campestris and Oryza sativa were used as subjects for test. Zoospores of the fungus were used to infect the plants with soil inoculation method, caudex injection method and foliage spray method. Both positive control (using P. aphanidermatum) and negative control (using sterile water) were set up in all the experiments. The results showed that no infection was found on the tested plants in soil inoculation experiments. In caudex injection test, callus grew around the wounded tissue in most of the plants. Brownish rottenness could be found only in the injected wounds in a few plants, probably caused by saprophytic bacteria or other fungi, and the germ-carrying plants grew normally. No abnormal appearance was found on the six crops in foliage spray test. It was demonstrated that P. guiyangense could hardly infect plants in nature, and was a safe and promising agent for mosquito biological control.
  • Mycosystema. 2008, 27(4): 574.
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    Phenolic compounds from field-grown Inonotus obliquus sclerotia (Chaga) consist mainly of hispidin analogs and melanins, and are thought to be the active constituents to treat several human diseases. In submerged cultures of the fungus, however, no information is currently available on the production of phenolic compounds and their corresponding pharmacological functions. In this study, phenolic compounds from Chaga and submerged cultures of the fungus were assayed for their composition and immune-stimulating effects. Phenolic compounds produced by I. obliquus in submerged cultures mostly consist of flavonoids, together with small amounts of hispidin analogs and melanins. This is quite contrary to the situation in Chaga, where flavonoids are determined as trace elements. Furthermore, phenolic compounds from Chaga show capacity about two-fold higher than those produced in submerged cultures in inhibiting cyclophosphamide-induced reduction of bodyweight, spleen index and viability of peripheral lymphocytes in test mice. Thus less production of hispidin analogs and melanins is likely to be responsible for less immune-stimulating effects in phenolic compounds from submerged cultures, and additional factors should be imposed during submerged cultures of I. obliquus to regulate biosynthesis of phenolic compounds directed to the composition similar to Chaga.
  • Mycosystema. 2008, 27(4): 582-586.
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    Trehalose, a non-reducing disaccharide, was separated for the first time from Ganoderma applanatum fruit body. The structure of trehalose was determined by electrospray ionization mass spectrometric and NMR data. The content of trehalose was determined by improved anthrone-sulphuric acid colorimetric method and it was 0.48% of dried weight of G. applanatum fruit body. Mannitol was simultaneously obtained during separation of trehalose from G. applanatum.
  • Mycosystema. 2008, 27(4): 587.
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    The antioxidant potency of components from Xylaria gracillima in submerged culture was investigated, employing various established in vitro systems, such as superoxide (O2?-) and hydroxyl (?OH) radical scavenging, reducing power, and ferrous ion chelating ability. Tocopherol (Ve), butylated hydroxytoluene (BHT) and ethylenediaminetetraacetic acid (EDTA) were used as positive controls. According to the results, components from X. gracillima in submerged culture showed significant effect on ferrous ion chelating ability, O2?- and ?OH radical scavenging ability at the range of concentration tested, and their highest antioxidant activities reached 89.72%, 70.90% and 77.46% respectively. The components also showed positive results of reducing power. These in vitro results suggested the possibility that components from X. gracillima in submerged culture could be effectively employed as an ingredient in healthy or functional food.
  • Mycosystema. 2008, 27(4): 594.
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    Pythium guiyangense is a mosquito pathogen, and has been proved to be a promising agent for biological control of mosquitoes. In order to develop the strains adaptable to different ecological environment having stable virulence to mosquito larvae, and being able to prolong the shelf life, an effort was made on transforming the fungus by using homologous or heterologous virulence genes. In this paper, a genetic transformation experiment of P. guiyangense mediated by Agrobacterium tumefaciens is reported. As a result, an A. tumefaciens mediated genetic transformation system was established successfully.
  • Mycosystema. 2008, 27(4): 601-603.
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  • Mycosystema. 2008, 27(4): 604-607.
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  • Mycosystema. 2008, 27(4): 608.
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  • Mycosystema. 2008, 27(4): 611.
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  • Mycosystema. 2008, 27(4): 614-618.
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  • Mycosystema. 2008, 27(4): 619.
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  • Mycosystema. 2008, 27(4): 622.
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  • Mycosystema. 2008, 27(4): 627.
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  • Mycosystema. 2008, 27(4): 630.
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