Studies of SCAR Markers Linked to Pollen Sterility Gene in Stylosanthes guianensis
HUANG Chun-qiong, ZHANG Zi-xiong, WANG Wen-qiang, LIU Guo-dao
Tropical Crops Genetic Resources Institute/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan Province 571101, China
Abstract：In the present study,the pollen fertility and sterility gene pool were constructed using BC1F1 individuals which got through continuous cross from S. guianens 1979 (pollen sterility) and S. guianensis ‘Reyan No.2’ (pollen fertility,recurrent parent) using ISSR technique and BAS (Bulked Segregate Analysis) method. The results showed that 11 ISSR (Inter-simple Sequence Repeat) specific markers of sterile gene pool were screened out using 100 ISSR primers. After individuals of population test,only 8 markers could be amplified in the pollen sterility individuals,which showed 8 markers existed certain linkage relationship with pollen sterility gene. Thus,the 8 markers were collected and sequenced to synthesize 8 SCAR pairs of primers respectively to amplify the individuals of the BC1F1 progeny,the results showed that the 8 markers could only be amplified in the pollen sterility individuals,indicating the 8 ISSR markers have been successfully converted into 8 SCAR markers which linked to pollen sterility gene. The research results provide the new theoretical basis for screening specific SCAR markers and establish stable male sterile line of S. guianensis, and lay the foundation for S.guianensis cross breeding.
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2020, Vol. 28 
