草地学报

›› 1998, Vol. 6 ›› Issue (1): 33-37.DOI: 10.11733/j.issn.1007-0435.1998.01.005

• 研究论文 • 上一篇    下一篇

热研二号柱花草组织培养研究

蒋昌顺1, 黄慧德1, 邹冬梅1, 陈守才2, 吴豪2   

  1. 1. 中国热带农业科学院农牧研究所, 海南省檐州市 571737;
    2. 华南热带农业大学热带作物生物技术国家重点实验室, 海南省儋州市 571737
  • 出版日期:1998-02-15 发布日期:1998-02-15
  • 基金资助:
    “九五”农业部畜牧业重点科研专题95-牧-02-02-14

Studies on Tissue Culture of Stylosanthes Guianensis Reyan Ⅱ

Jiang Changshun1, Huang Huide1, Zou Dongmei1, Chen Shoucai2, Wu Hao2   

  1. Tropical Field Crops and Animal Husbandry Researeh Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737
  • Online:1998-02-15 Published:1998-02-15

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摘要： 研究结果表明,利用热研二号柱花草无菌苗的子叶为外植体,在M2培养基(MS基本成分+0.8%琼脂+3.0%蔗糖+1.0mg/L萘乙酸+4.0mg/L激动素,pH6.0)培养可诱导分化出愈伤组织和枝条。1月龄枝条在M7培养基(1/2MS基本成分+0.8%琼脂+1.0%蔗糖+0.5mg/L萘乙酸+0.05%活性炭,pH6.0)培养可诱导生根,生根率60%,获得了具有根、茎、叶的完整柱花草组培植株。25和50μg/ml浓度的卡那霉素均能完全抑制子叶形成愈伤组织和愈伤组织再分化。

关键词: 柱花草, 组织培养

Abstract: Studies on tissue culture of Stylosanthes guianensis REYAN Ⅱshow that cotyledon is apromising explant.Callus and shoots can be induced from cotyledon cultured on the medium containingbasic salt of MS, 0.8% agar, 3.0% sucrose, 1.0mg/L NAA and 4.0mg/L BA, pH6.0.Rooting canbe induced from one-month-old shoots cultrued on the mediurn containing half strength MS basic salt,0.8% agar, 1.0% sucrose, 0.5mg/L NAA, and 0.005% active cbarcoal, pH6.0 and the rooting rateis 60%, 25ug/ml, 50ug/ml of Kanamycin inhibits callus formation and redifferentiation respectively.

Key words: Stylosanthes spp., Tissue Culture