草地学报

›› 2001, Vol. 9 ›› Issue (2): 83-86.DOI: 10.11733/j.issn.1007-0435.2001.02.002

• 研究论文 • 上一篇    下一篇

耐盐苜蓿与敏盐苜蓿RAPD多态性研究

杨青川1, 孙杰2, 韩国栋2   

  1. 1. 中国农业科学院畜牧研究所, 北京, 100094;
    2. 内蒙古农业大学生态环境学院, 呼和浩特, 010018
  • 收稿日期:2000-11-10 修回日期:2001-01-14 出版日期:2001-05-15 发布日期:2001-05-15
  • 作者简介:杨青川(1966- ),男,副研,主要从事牧草常规育种与生物技术育种研究,主持国家自然科学基金、"九五”科技攻关课题、农科院重大推广等项目
  • 基金资助:
    国家自然科学基金项目(39870560)资助

Studies on RAPD Polymorphic in Salt-Tolerant Alfalfa and Salt Sensitive Alfalfa

YANG Qing-chuan1, SUN Jie2, HAN Guo-dong 2   

  1. 1. Institute of Animal Science, CAAS, Beijing 100094, China;
    2. Ecological Environment College, Inner Mongolia Agriculture University, Huhhot 010018, China
  • Received:2000-11-10 Revised:2001-01-14 Online:2001-05-15 Published:2001-05-15

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摘要： 以耐盐苜蓿和敏盐苜蓿为材料,通过RAPD反应,对8组的160条引物进行筛选.结果表明,经过5轮筛选,OPA组6条引物、OPD组9条引物、OPE组6条引物、OPF组7条引物、OPH组5条引物、OPC组9条引物、OPG组2条引物、OPN组7条引物共51条都可作为多态性引物,其中以OPA-17、OPD-5、OPD-7等3条引物的DNA多态性最稳定.在"中苜一号”DNA组上,OPA-17可在2200bp处产生一条多态性谱带,并在380bp出现谱带缺失现象.OPD-5可在3500bp处形成一条清晰度极高的谱带,OPD-7则在650bp处无谱带产生.这3条引物所检测出的耐盐苜蓿与敏盐苜蓿具有稳定的DNA多态性,该多态性与其耐盐性状有关.

关键词: 苜蓿, RAPD, DNA多态性, 耐盐性状

Abstract: The salt tolerant alfalfa and the salt sensitive alfalfa are used as material of extracting DNA,through RAPD reaction,we can select the primer of polymorphic DNA from 160 lines in eight groups' primers.After five turns,51 lines being of polymorphic DNA have been found.Among them OPA 17 OPD 5 and OPD 7 are three lines of the most stable polymorphic DNA.On the DNA of Medicago sativa L.“Zhongmu NO.1”,OPA 17 is able to produce a band of polymorphic DNA in 2200 bp DNA size and lose a band in the 380 bp;OPD 5 can lead a clear band in the 3500 bp; OPD 7 has lack of the band in 650 bp DNA point.The polymorphic DNA having stable is inspected by the three lines of primer.They maybe relact with the salt tolerance in Medicago sativa L.“Zhongmu NO.1”.

Key words: Alfalfa, Primer, RAPD, DNA polymorphic, Salt tolerace

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