草地学报

›› 2006, Vol. 14 ›› Issue (3): 298-300.DOI: 10.11733/j.issn.1007-0435.2006.03.021

• 研究报告 • 上一篇    

柱花草SgNCED1基因的克隆及功能分析

杨锦芬, 郭振飞   

  1. 华南农业大学生命科学学院草业生物技术实验室, 广州, 510642
  • 收稿日期:2006-06-22 修回日期:2006-07-10 出版日期:2006-08-15 发布日期:2006-08-15
  • 作者简介:杨锦芬(1978- ),广东广州人,博士研究生,主要从事草业生物技术研究
  • 基金资助:
    广东省自然科学基金重点项目(04105978);和国家转基因植物研究专项(JY03-B-32-02)

Cloning and Functional Analysis of a 9-Cis-Epoxycarotenoid Dioxygenase Gene(SgNCED1) from Stylosanthes Guianensis

YANG Jin-fen, GUO ZHen-fei   

  1. Biotechnology Laboratory for Turfgrass and Forages, College of Life Science, South China Agriculture University, Guangzhou, Guangdong Province, 510642, China
  • Received:2006-06-22 Revised:2006-07-10 Online:2006-08-15 Published:2006-08-15

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摘要： 脱落酸(ABA)作为一种调控生长发育的激素,对植物多种生理过程起着调控作用。9-顺式环氧类胡萝卜素双加氧酶(9-cis-epoxycarotenoid dioxygenase,NCED)是高等植物ABA生物合成途径的关键酶。NCED基因受逆境胁迫诱导,导致逆境ABA的积累。研究NCED基因克隆、表达,调控ABA的生物合成,是提高植物抗逆性的重要途径。柱花草(Stylosanthesgu ianensis)是一种热带和亚热带地区广泛种植的耐旱草种。

关键词: 柱花草, ABA, NCED, 逆境胁迫, 转基因烟草

Abstract: Abscisic acid(ABA)plays important roles in the adaptation of plants to a variety of environmental stresses.The oxidative cleavage of cis-epoxy carote-noids catalyzed by 9-cis-epoxy carotenoid dioxy genase(NCED)isthe key step in the regulation of abscisic acid(ABA)biosynt hesis in higher plants.The environmental stresses induce the expression of NCED and there by regulate the endogenous ABA level.Stylosanthes guianensis is animportant drought-tolerant pasture legume of the tropical and subtropical regions.The objectives of this studywere to clone the NCED gene from S.guianensis and to analyze its function in response to abiotic stresses as well as to provide a method for the molecular breeding of stress-to lerant crops.The results are sum marized inthe following 4 sections.

Key words: Stylosanthes guianensis, Abscisic acid, 9-cisepoxycarotenoid dioxygenase(NCED), Environ-mental stresses, Transgenic tobacco

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