草地学报

›› 2014, Vol. 22 ›› Issue (2): 312-318.DOI: 10.11733/j.issn.1007-0435.2014.02.016

• 研究论文 • 上一篇    下一篇

盐碱胁迫对紫花苜蓿种子发芽的协同影响

蔺吉祥1, 高战武2, 王颖3, 于兴洋4, 邵帅1, 李晓宇4   

  1. 1. 东北林业大学盐碱地生物资源环境研究中心 东北油田盐碱植被恢复与重建教育部重点实验室, 黑龙江 哈尔滨 150040;
    2. 白城师范学院地理系, 吉林 白城 137000;
    3. 吉林建筑大学 松辽流域水环境教育部重点实验室, 吉林 长春 130118;
    4. 中国科学院东北地理与农业生态研究所, 吉林 长春 130024
  • 收稿日期:2013-06-06 修回日期:2013-09-23 出版日期:2014-04-15 发布日期:2014-04-21
  • 通讯作者: 高战武
  • 作者简介:蔺吉祥（1985-），男，黑龙江哈尔滨人，讲师，主要从事种子生态学与植物逆境生理学研究，E-mail：jixiang851012@gmail.com
  • 基金资助:
    中央高校基本科研业务费专项资金（DL12BA32）（2572014EA04）；哈尔滨市科技创新人才专项基金（2013RFQXJ022）资助

Synergistic Effects of Salt and Alkaline Stresses on the Seed Germination of Medicago sativa

LIN Ji-xiang1, GAO Zhan-wu2, WANG Ying3, YU Xing-yang4, SHAO Shuai1, LI Xiao-yu4   

  1. 1. Alkali Soil Natural Environmental Science Center, Northeast Forestry University/ Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin, Heilongjiang Province 150040, China;
    2. Geography Department of Baicheng Normal University, Baicheng, Jilin Province 137000, China;
    3. Key Laboratory of Songliao Aquatic Environment, Ministry of Education, Jilin Jianzhu University, Changchun, Jilin Province 130118, China;
    4. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin Province 130012, China
  • Received:2013-06-06 Revised:2013-09-23 Online:2014-04-15 Published:2014-04-21

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摘要： 以紫花苜蓿（Medicago sativa）为材料，在实验室内模拟土壤复杂盐碱逆境条件，将2种中性盐NaCl, Na2SO4与2种碱性盐NaHCO3, Na2CO3以不同的摩尔比例混合，按碱性盐比例递增的顺序共设置A～F组，同时每个组又设置6种盐浓度（24～168 mM），涵盖Na+浓度为32～192 mM，pH为7.03～10.68范围的盐碱生境，试验共模拟出36种盐度和碱度（pH值）各不相同的盐碱条件，并对胁迫下苜蓿种子发芽指标进行了测定。结果表明：低浓度胁迫对苜蓿种子发芽有一定的促进作用，在各胁迫处理组下苜蓿种子的发芽指标（发芽率、发芽势、发芽指数和活力指数）均随着盐浓度的增加不断下降，且碱性盐比例越大下降越明显，而平均发芽天数逐渐增加。胚根与胚芽生长表现出与发芽相似的变化规律，并且胚根适应盐碱能力更强。因此，复合盐碱胁迫不同于单独的盐或碱胁迫，一旦浓度较高时，盐和碱的交互作用更明显，二者存在明显的协同作用，加剧了对植物的伤害。

关键词: 紫花苜蓿, 种子, 盐碱胁迫, 发芽率, 发芽势

Abstract: Alfalfa (Medicago sativa) was taken as research material. Saline stress was simulated by mixing NaCl and Na2SO4 in different molar ratios. Similarly, alkaline stress was simulated by mixing NaHCO3 and Na2CO3 with different molar ratios. Six groups (A to F) with different Na+ concentrations and pH values were designed. Results showed that the germination rate of alfalfa seed was promoted by lower concentration. The germination indices of alfalfa seed (germination rate, germination energy, germination index and vigor index) were all decreased with the increase of salinity and the decreasing degree were more obvious with increasing the proportion of alkaline salt, whereas mean germination days were increased. Radicle was more tolerant to saline and alkaline stresses compared with embryo. These data indicated that the mixed salinity-alkalinity stresses were different from either saline or alkaline stress. The interactions of saline and alkaline stresses increased with the increase of salt concentration. Plant was damaged severely by the synergy of both stresses.

Key words: Medicago sativa, Seed, Salt and alkaline stress, Germination rate, Germination potential

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