草地学报 ›› 2017, Vol. 25 ›› Issue (2): 315-321.DOI: 10.11733/j.issn.1007-0435.2017.02.014

• 研究论文 • 上一篇    下一篇

内蒙古荒漠草原土壤可培养真菌的群落结构和空间分布分析

贾美清1, 黄静2, 孟元2, 韩国栋3, 孙胜3, 张国刚2, 杨英花2   

  1. 1. 天津师范大学 天津市水资源与水环境重点实验室, 天津 300387;
    2. 天津师范大学生命科学学院, 天津 300387;
    3. 内蒙古农业大学草原与资源环境学院, 内蒙古 呼和浩特 010019
  • 出版日期:2017-04-15 发布日期:2017-06-25
  • 通讯作者: 张国刚,E-mail:zangguogang@163.com
  • 作者简介:贾美清(1978-),女,山西人,硕士,实验师,E-mail:jiameiqing@mail.nankai.edu.cn
  • 基金资助:

    国家自然科学基金(31500365,31100330,31270502);天津市自然科学基金(12JCYBJC19700);天津市科技支撑基金项目(15ZCZDSF00410)资助

Analysis of Cultivable Fungal Community Structure and Spatial Distribution in Desert Steppe, Inner Mongolia, China

JIA Mei-qing1, HUANG Jing2, MENG Yuan2, HAN Guo-dong3, SUN Sheng3, ZHANG Guo-gang2, YANG Ying-hua2   

  1. 1. Tianjin Key Laboratory of Water Resource and Water Environment, Tianjin Normal University, Tianjin 300387, China;
    2. College of Life Sciences, Tianjin Normal University, Tianjin 300387, China;
    3. College of Grsaalang, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China
  • Online:2017-04-15 Published:2017-06-25

摘要:

为了揭示荒漠草原土壤可培养真菌的群落结构和空间分布,以实现利用微生物对环境变化的敏感性对退化草地提前预测和有效管理,采用稀释平板涂布法和18S rRNA分子鉴定技术对内蒙古荒漠草原土壤可培养真菌的群落结构、多样性、空间分布及其与土壤环境因子的相关性进行分析。结果表明:可培养真菌在0~30 cm土层的数量为26.65×104~90.69×104 CFU·g-1,各土层的真菌分布次序为:0~10 cm > 10~20 cm > 20~30 cm。分离到的真菌分别属于14个属,其中青霉菌属是优势菌属,占0~30 cm可培养真菌总数的48.76%, 其次是曲霉属,占可培养真菌总数的10%。在0~10 cm土层,可培养真菌的物种丰富度和Shannon-Wiener 指数(H')最高。可培养真菌的数量与含水量、速效钾和铵态氮呈显著正相关关系(P<0.05);与有机质含量呈极显著正相关关系(P<0.01),与pH值呈显著负相关关系(P<0.05);与硝态氮和速效磷无显著相关性。

关键词: 荒漠草原, 可培养真菌, 18S rRNA, 多样性, 空间分布

Abstract:

In order to assess the community structure and spatial distribution of cultivable fungi in desert steppe, Inner Mongolia, China, dilution plate method and 18S rRNA molecular identification technique were used to study the community composition, distribution and diversity of cultivable fungi. Pearson's correlation was used to analyze the relationships between the quantity of fungi and soil physical and chemical factors. The quantity of cultivable fungi was 26.65×104~90.69×104 CFU·g-1 in 0~30 cm soil depth. The distribution trend of cultivable fungi was 0~10 cm > 10~20 cm > 20~30 cm. Cultivable fungi isolated from desert steppe soil belonged to 14 genus including Penicillium, Fusarium, and so on. The dominant strains were Penicillium and Aspergillus, which relative abundances in 0~30 cm soil layer were 48.76% and 10%, respectively. Compared with other two soil layers, the species richness and Shannon-Weiner Index(H') of cultivable fungi were the highest in 0~10 cm soil depth. The distribution of cultivable fungi had a significant correlation with water content, NH4+-N, available potassium, and soil organic matter content, while it had a significantly negative correlation with pH value. It had no significant correlation with available phosphorus and NO3--N content.

Key words: Desert steppe, Cultivable fungi, 18S rRNA, Community structure, Spatial distribution

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