草地学报

草地学报 ›› 2018, Vol. 26 ›› Issue (6): 1409-1415.DOI: 10.11733/j.issn.1007-0435.2018.06.017

• 研究论文 • 上一篇    下一篇

60Co-γ辐射对三种盐碱胁迫下稗草种子萌发的影响

李红1, 李波2, 杨曌1   

  1. 1. 黑龙江省畜牧研究所, 黑龙江 齐齐哈尔 161005;
    2. 齐齐哈尔大学生命科学与农林学院, 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江 齐齐哈尔 161006
  • 收稿日期:2018-09-03 修回日期:2018-12-24 出版日期:2018-12-15 发布日期:2019-01-28
  • 通讯作者: 李波
  • 作者简介:李红(1960-),女,山东莱芜人,研究员,主要从事牧草与饲料作物育种与栽培研究,E-mail:hljlihong@163.com
  • 基金资助:
    黑龙江省应用技术研究与开发计划重大项目（GA15B105-5）；黑龙江省省属高等学校基本科研业务费科研项目（135209267，YSTSXK201886）资助

Effect of 60Co-γ Radiation on Seeds Germination of Echinochloa crusgalli under Saline-alkali Stress

LI hong1, LI Bo2, YANG Zhao1   

  1. 1. Animal Science Institute of Heilongjiang, Qiqihar, Heilongjiang Province 161005, China;
    2. College of Agriculture, Forestry and Life Sciences, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China
  • Received:2018-09-03 Revised:2018-12-24 Online:2018-12-15 Published:2019-01-28

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摘要： 为确定有助于提升稗草种子耐盐性的60Co-γ射线辐射剂量，对不同浓度NaCl、NaHCO3和NaCl+NaHCO3胁迫下种子发芽率的变化进行分析，确定了3种盐碱的半致死浓度，同时对不同剂量辐射处理的种子进行3种盐碱胁迫处理，比较辐射种子在3种盐碱胁迫下种子萌发和芽苗生长的影响。结果表明，3种盐碱胁迫的半致死浓度分别为280 mmol·L-1NaCl、80 mmol·L-1NaHCO3和280 mmol·L-1NaCl+70 mmol·L-1NaHCO3；适宜辐射剂量可以促进NaCl胁迫下种子的发芽率、发芽势、发芽指数、活力指数及芽苗总长、胚芽长、根长、鲜重、干重、相对含水量和NaCl+NaHCO3胁迫下种子的发芽率、发芽指数和芽苗干重，NaHCO3胁迫抑制种子萌发和芽苗的生长，增加芽苗鲜重、干重和相对含水量。不同辐射剂量对3种盐碱胁迫种子萌发和芽苗生长各指标的影响不同，隶属函数综合分析，辐射剂量在50，100和150 Gy能提高稗草种子耐NaCl能力，但辐射不能提高其耐NaHCO3和混合盐碱能力。

关键词: 稗草种子, 60Co-γ辐射, 盐碱胁迫, 种子萌发

Abstract: In order to explore the 60Co-γ rays radiation dose of improving the salt tolerance of Echinochloa crusgalli seeds,the semi-lethal concentrations of three saline alkaloids were determined by analyzing the changes of seed germination rate under NaCl,NaHCO3 and NaCl+NaHCO3 stress. At the same time,3 kinds of saline-alkali stress were applied to the seeds treated with different doses. Effects of different radiation doses on seed germination and seedling growth under 3 kinds of saline-alkali stress were compared. The results showed that the semi-lethal concentrations of three saline-alkali stress were 280 mmol·L-1 NaCl,80 mmol·L-1 NaHCO3 and 280 mmol·L-1 NaCl+70 mmol·L-1 NaHCO3,respectively. The suitable radiation dose promoted germination rate,germination potential,germination index,vigor index,total length of bud seedling,embryo length,root length,fresh weight,dry weight and relative water content under NaCl stress and germination rate,germination index and dry weight of seedlings under NaCl+NaHCO3 stress. Seed germination and seedling growth were inhibited and seedling fresh weight,dry weight and relative water content were increased under NaHCO3 stress. Different radiation doses had different effects on seed germination and seedling growth of 3 kind of saline alkali stresses. Comprehensive analysis of membership functions showed that radiation dose of 50,100 and 150 Gy enhanced the NaCl tolerance of Echinochloa crusgalli seeds. However,radiation did not enhance its ability to resist NaHCO3 and mixed saline-alkali.saline.

Key words: Echinochloa crusgalli ‘Chaomu No.1’, 60Co-γ radiation, Saline-alkali stress, Seed germination

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